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Species: Homo sapiens
Genetic Insert: RelA
Vector Backbone Description: Backbone Marker:Matthias et al, Nucleic Acid Research (1989).; Backbone Size:5120; Vector Backbone:modified pEV3S (pLG-033); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16135789
Comments: FOr more details for this expression construct as well as other mutants, please contact Dr Lin-Feng CHen ([email protected])
Proper citation: RRID:Addgene_24153 Copy
Species: Mus musculus
Genetic Insert: nAChR beta2 WT
Vector Backbone Description: Backbone Size:5472; Vector Backbone:pCIneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14684858
Proper citation: RRID:Addgene_24272 Copy
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:7000; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427958
Comments: pUltra was cloned by Yildirim Dogan ([email protected]). It is described in the associated article (PMID 22427958) and also in PMID 24630793.
This is a 3rd generation Lentiviral vector with an internal Ubiquitinc Promoter.
By cloning into the compatible cloning sites* (XbaI and BamHI) downstream of EGFP-P2A, you get a bi-cistronic expression of EGFP and the gene of interest. You can clone a second gene of interest into the NheI/bclI**donwstream of EGFP-P2A-gene1-T2A and get a multi-cistronic expression of all three genes.
* compatible cloning site (ccs): you can pcr amplifiy your gene of interest with a forward primer including one of these cutting sites: SpeI or NheI or XbaI and a reverse primer with one these cutting sites: BglII or BamHI or bclI. The PCR product is now compatible with the first site as well the 2nd site. "You can mix and match".
** for bclI the vector has to be amplified by dam-methylation defective E coli strains (e.g. SC110 Stratagene).
Further advantage of the vector is, that you can simultaneously do RNAi by cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR. Plus while integration the RNAi cassette gets doubled, since the 5´LTR is dublicated while reverse transcripton from the 3´-LTR
***P2A: 3984-4046 bp and T2A: 4062-4124 bp
Proper citation: RRID:Addgene_24129 Copy
Species: Homo sapiens
Genetic Insert: E2F1 E132
Vector Backbone Description: Backbone Size:6200; Vector Backbone:pCMVNeoBam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8405995
Comments: Recloned from D. Cress CMV Invitrogen
Proper citation: RRID:Addgene_24224 Copy
Species: Homo sapiens
Genetic Insert: E2F1
Vector Backbone Description: Backbone Size:6200; Vector Backbone:pCMVHANeoBam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8622649
Comments: Insert check with SalI, which leads to a 1.3kb fragment + vector. E2F1 cDNA can be removed by BamHI digest.
This plasmid contains an E356K mutation within the E2F1 insert. Please note that other mutations may exist and it is unclear whether this mutation affects plasmid function.
Proper citation: RRID:Addgene_24225 Copy
Species: Homo sapiens
Genetic Insert: SUZ12
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pCMVHANeoBam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15385962
Proper citation: RRID:Addgene_24232 Copy
Species: Homo sapiens
Genetic Insert: alpha catenin
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Rimm Lab Plasmid #221
Proper citation: RRID:Addgene_24194 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-2AB Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Rimm Lab Plasmid #351
Proper citation: RRID:Addgene_24204 Copy
Species: HIV
Genetic Insert: Nef
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCl; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11298454
Comments: NL4-3 is a wild-type Nef allele.
Proper citation: RRID:Addgene_24162 Copy
Species: Homo sapiens
Genetic Insert: NFATc1
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9106655
Proper citation: RRID:Addgene_24219 Copy
Species: Homo sapiens
Genetic Insert: E2F5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pCMVNeoBam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: cause of the cloning a BamHI cut leads to 1.5kp and 6.5kb
Proper citation: RRID:Addgene_24213 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Clontech (Unmodified); Backbone Size:5200; Vector Backbone:pIRESpuro-modified with gateway cassette by recombination clonin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20096707
Comments: EGFP was cloned into pENTR-D (invitrogen). The EGFP open reading frame was transferred from pENTRD- EGFP to a gateway modified pIRES-Puro plasmid via LR recombination reaction.
Proper citation: RRID:Addgene_24176 Copy
Species: Homo sapiens
Genetic Insert: UV irradiation resistance-associated gene
Vector Backbone Description: Backbone Marker:sigma-aldrich; Backbone Size:6299; Vector Backbone:p3xFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18843052
Proper citation: RRID:Addgene_24292 Copy
Species: Aequorea victora (modified) / synthetic
Genetic Insert: 7xTcf-eGFP // SV40-mCherry
Vector Backbone Description: Backbone Marker:Naldini/Trono (Addgene #12252); Backbone Size:6132; Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20186325
Comments: TCF repeat sequence is AGATCAAAGG
This plasmid requires a 2nd generation lentiviral packaging system.
Proper citation: RRID:Addgene_24304 Copy
Species: Aequorea victoria (modified) / Streptomyces alboniger
Genetic Insert: 7xTcf-eGFP // SV40-PuroR
Vector Backbone Description: Backbone Marker:Naldini/Trono (Addgene #12252); Backbone Size:6132; Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20186325
Comments: TCF repeat sequence is AGATCAAAGGgg.
This plasmid requires a 2nd generation lentiviral packaging system.
Proper citation: RRID:Addgene_24305 Copy
Species: Homo sapiens
Genetic Insert: Beclin1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pCDNA4/TO-3*flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24389 Copy
Genetic Insert: mCD8-GFP
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pUAS-mCD8-GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The FRT-stop-FRT cassette, with additional BglII/NotI restriction sites, was PCR amplified from genomic DNA of UAS-FRT-stop-FRT-shibire (ts) flies and cloned into pUAST-mCD8-gfp to generate pUAST-FRT-stop-FRT-mCD8-gfp (pUAS>stop>mCD8-GFP)
Proper citation: RRID:Addgene_24385 Copy
Species: Mus musculus
Genetic Insert: EF1alpha-betacatenin(deltaGSK) // SV40-PuroR
Vector Backbone Description: Backbone Marker:Naldini/Trono (Addgene #12252); Backbone Size:6074; Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20186325
Comments: This plasmid requires a 2nd generation lentiviral packaging system.
Proper citation: RRID:Addgene_24313 Copy
Species: Homo sapiens
Genetic Insert: Beclin1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pCDNA4/TO-3*flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24390 Copy
Species: Homo sapiens
Genetic Insert: Beclin1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pCDNA4/TO-3*flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24393 Copy
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