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Species: Homo sapiens
Genetic Insert: BCRn-CAGEn (Npu)
Vector Backbone Description: Backbone Size:4664; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38977789
Comments: Engineered NpuN intein (Caged NpuN) fused to the 3'-end of BCR (1-928 aa). FKBP* domain and various NLS sequences (SV40, Nucleoplasmin, c-Myc) fused to the 3'-end of Caged NpuN.
Proper citation: RRID:Addgene_241419 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Size:5378; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note: This plasmid contains a 33 amino acid insertion that adds repetitions of the signal peptide of ACE2. It is not known how this impacts the plasmid's function, but it could still be utilized to assess ACE2 cleavage by TMPRSS2
The sequences encoding ACE2 mutants with a C-terminal FLAG tag were generated in two steps. In step 1, ACE2 mutant inserts were generated by overlap-extension PCR with specific primers. In step 2, the mutated ACE2 PCR insert was inserted into pcDNA 3.1 vector using the NEBuilder HiFi DNA Assembly kit. The integrity of all PCR-amplified sequences was confirmed by automated sequence analysis.
Proper citation: RRID:Addgene_240608 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:rAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38915722
Comments: Allen Institute plasmid alias: CN4211
Proper citation: RRID:Addgene_229936 Copy
Species: Homo sapiens
Genetic Insert: Mutant p300 core-dCas9-EGFP fusion protein
Vector Backbone Description: Backbone Size:15642; Vector Backbone:PiggyBac dCas9-VPR; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, PiggyBac Transposon; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_226427 Copy
Species: Thermotoga maritima
Genetic Insert: tRNase Z
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40617857
Proper citation: RRID:Addgene_242436 Copy
Species: Homo sapiens
Genetic Insert: TRAF3
Vector Backbone Description: Backbone Marker:Fu lab; Vector Backbone:pSCM167-VenusFlag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_241875 Copy
Species: Homo sapiens
Genetic Insert: DHX58
Vector Backbone Description: Backbone Marker:Fu lab; Vector Backbone:pSCM167-VenusFlag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_241879 Copy
Species: Mus musculus
Genetic Insert: murine Nr1d1 promoter+miRFP713-NLS-Pest-Reporter
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLenti6-puromycin; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Exchanged fluorophore from pL6puro-JeT-pmRevErbA-Venus-NLS-Pest. Insertion of JeT promoter confers moderate expression, depending on cell type. For higher brightness consider SV40 containing variants. Please visit https://doi.org/10.1101/2025.06.30.661926 for bioRxiv preprint.
Proper citation: RRID:Addgene_240110 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Merck Millipore; Vector Backbone:Simplicon Cloning Vector; Vector Types:Mammalian Expression, Self-amplifying RNA; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2024.09.24.614636 for the bioRxiv preprint.
Proper citation: RRID:Addgene_242414 Copy
Species: Homo sapiens
Genetic Insert: EXOC3 (-1557 to-1046)
Vector Backbone Description: Vector Backbone:pCR2.1-Topo; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39658038
Comments: The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
Proper citation: RRID:Addgene_226446 Copy
Species: Synthetic
Genetic Insert: 30x 147-bp Widom 601
Vector Backbone Description: Backbone Marker:Erik Jorgensen (Addgene plasmid #73738); Backbone Size:2438; Vector Backbone:pMLS280; Vector Types:DNA amplification; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40291647
Comments: Cloning carried out as in: Dyer PN, Edayathumangalam RS, White CL, Bao Y, Chakravarthy S, Muthurajan UM, Luger K. Reconstitution of nucleosome core particles from recombinant histones and DNA. Methods Enzymol. 2004;375:23-44. doi: 10.1016/s0076-6879(03)75002-2. PMID: 14870657.
DNA purification as in: Paintsil EA, Morrison EA. Preparation of Recombinant Histones and Widom 601 DNA for Reconstitution of Nucleosome Core Particles. Methods Mol Biol. 2023;2599:163-175. doi: 10.1007/978-1-0716-2847-8_12. PMID: 36427149.
Nucleosome core particle reconstitutions largely as in: Hammonds EF, Morrison EA. Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA. Methods Mol Biol. 2023;2599:177-190. doi: 10.1007/978-1-0716-2847-8_13. PMID: 36427150. Exception: Suggest reconstitution molar ratios of 1:1:2 DNA:H3/H4-tetramer:H2A/H2B-dimer.
Please visit https://doi.org/10.1101/2025.04.09.647968 for bioRxiv preprint.
Proper citation: RRID:Addgene_240634 Copy
Species: Synthetic
Genetic Insert: VP16
Vector Backbone Description: Backbone Size:6774; Vector Backbone:pPB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40500178
Proper citation: RRID:Addgene_241843 Copy
Species: Homo sapiens
Genetic Insert: CRAF
Vector Backbone Description: Vector Backbone:pFC223C NanoLuc(R) CMV-Neo BiBRET-Balanced Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_236870 Copy
Species: Lactiplantibacillus plantarum
Genetic Insert: Lactate racemase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39679682
Proper citation: RRID:Addgene_232792 Copy
Species: Homo sapiens
Genetic Insert: Fab (antigen binding fragment) anti-nucleocapsid protein of SARS-COV viruses 1 and 2
Vector Backbone Description: Backbone Marker:Lamminmäki Group, Department of Life Technologies, University of Turku; Backbone Size:6000; Vector Backbone:pLK06Hpp; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40885348
Proper citation: RRID:Addgene_239861 Copy
Species: Synthetic
Genetic Insert: H2B-iRFP
Vector Backbone Description: Vector Backbone:pminiTol2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40906841
Proper citation: RRID:Addgene_240802 Copy
Species: SARS-CoV-2
Genetic Insert: GFP-Nsp3(1-111)
Vector Backbone Description: Backbone Size:5251; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39464032
Proper citation: RRID:Addgene_242947 Copy
Species: Synthetic
Genetic Insert: CIBN-eGFP
Vector Backbone Description: Vector Backbone:pminiTol2; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40906841
Proper citation: RRID:Addgene_240804 Copy
Species: inflluenza A virus
Genetic Insert: mvRNA NP71.2
Vector Backbone Description: Vector Backbone:pPol I; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_240808 Copy
Species: Lactiplantibacillus plantarum
Genetic Insert: Pyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3829; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39679682
Proper citation: RRID:Addgene_232793 Copy
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