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Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2799; Vector Backbone:BASIC_SEVA_47.10; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36381610
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178930 Copy
Species: Caenorhabditis elegans
Genetic Insert: UNC-7
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7737; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179220 Copy
Species: Synthetic
Genetic Insert: IDT-Cas9_v2
Vector Backbone Description: Vector Backbone:pRDA_085; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179092 Copy
Species: Homo sapiens
Genetic Insert: APOBEC1-YTHmut-T2A-GFP
Vector Backbone Description: Backbone Marker:Adam Karpf (Feng Zhang); Backbone Size:11775; Vector Backbone:TLCV2 (lentiCRISPR v2); Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology, Inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35081365
Proper citation: RRID:Addgene_178950 Copy
Species: Synthetic
Genetic Insert: eCas9-1.1
Vector Backbone Description: Vector Backbone:pRDA_085; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179085 Copy
Species: Synthetic
Genetic Insert: Cas9-HF
Vector Backbone Description: Vector Backbone:pRDA_085; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179084 Copy
Species: Synthetic
Genetic Insert: Cas9-VQR
Vector Backbone Description: Vector Backbone:pRDA_085; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179088 Copy
Genetic Insert: AtTIR1(F74G)
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34865044
Proper citation: RRID:Addgene_178945 Copy
Species: Homo sapiens
Genetic Insert: APOBEC1-YTH-T2A-GFP
Vector Backbone Description: Backbone Marker:Feng Zhang lab; Backbone Size:11777; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology, Inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35081365
Proper citation: RRID:Addgene_178949 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2607; Vector Backbone:BASIC_SEVA_67.10; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:36381610
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178940 Copy
Genetic Insert: Cargo EGFP with AttP Bxb1 site for in-frame fusion
Vector Backbone Description: Vector Backbone:pMC.BESPX; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36424489
Comments: Please visit https://doi.org/10.1101/2021.11.01.466786 for bioRxiv preprint.
Proper citation: RRID:Addgene_179116 Copy
Genetic Insert: pCAG-NLS-SscINT
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36424489
Comments: Please visit https://doi.org/10.1101/2021.11.01.466786 for bioRxiv preprint.
Proper citation: RRID:Addgene_179113 Copy
Genetic Insert: pCAG-NLS-BceINT
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36424489
Comments: Please visit https://doi.org/10.1101/2021.11.01.466786 for bioRxiv preprint.
Proper citation: RRID:Addgene_179112 Copy
Genetic Insert: TP901-1 Integrase
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36424489
Comments: Please visit https://doi.org/10.1101/2021.11.01.466786 for bioRxiv preprint.
Proper citation: RRID:Addgene_179110 Copy
Species: Synthetic
Genetic Insert: GST-His8-GFP-nanobody
Vector Backbone Description: Backbone Marker:Cytiva; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178975 Copy
Species: Homo sapiens
Genetic Insert: FOXO3a
Vector Backbone Description: Backbone Size:4968; Vector Backbone:pGex 4T3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10102273
Comments: See author's sequence and map.
Proper citation: RRID:Addgene_1790 Copy
Species: Synthetic
Genetic Insert: Sec61b-V5-TurboID
Vector Backbone Description: Backbone Marker:Tong-Chuan He laboratory; Backbone Size:35725; Vector Backbone:pAdTrack-CMV, pAdEasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34471136
Comments: Sec61b-V5-TurboID_pcDNA5 is Addgene plasmid # 166971.
To generate adenovirus, digest with PacI and perform transfection to HEK293 cells according to Luo et al (PMID:17546019) procedure #14.
Please note: The plasmid contains a 1650bp insertion just downstream of the Left Arm compared to the depositor's provided sequence. This difference is not known to affect plasmid function.
Proper citation: RRID:Addgene_179143 Copy
Species: Danio rerio
Genetic Insert: Glial fibrillary acidic protein promoter
Vector Backbone Description: Backbone Size:3323; Vector Backbone:pminiTol2; Vector Types:Tol2 mediated recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28882119
Proper citation: RRID:Addgene_178963 Copy
Species: Danio rerio
Genetic Insert: Glial fibrillary acidic protein promoter
Vector Backbone Description: Backbone Size:3323; Vector Backbone:pminiTol2; Vector Types:Tol2 mediated recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28882119
Proper citation: RRID:Addgene_178964 Copy
Species: Synthetic
Genetic Insert: ABE8e
Vector Backbone Description: Vector Backbone:pRDA_256; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179097 Copy
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