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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Nucleocapsid (N protein)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4760; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Expresses SARS CoV-2 Nucleoprotein (N gene; nucleocapsid) in insect cells with TEV-cleavable N-terminal His6 tag. Express 4-5 days at 27C. Yield from NiNTA column is approximately 100 mg/L of culture using ExpiSF expression system.
Proper citation: RRID:Addgene_179696 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:9184; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179576 Copy
Genetic Insert: xCas9
Vector Backbone Description: Vector Backbone:pFREE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35007422
Proper citation: RRID:Addgene_179579 Copy
Species: Synthetic
Genetic Insert: [sd4U] UGI-TALE3-DddA-G1397-N wildtype
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35379961
Comments: araC
Proper citation: RRID:Addgene_179690 Copy
Species: Synthetic
Genetic Insert: [sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35379961
Comments: araC
Proper citation: RRID:Addgene_179691 Copy
Species: Homo sapiens
Genetic Insert: v-myc myelocytomatosis viral oncogene homolog (avian)
Vector Backbone Description: Backbone Size:4622; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18287077
Proper citation: RRID:Addgene_17966 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5767; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179573 Copy
Species: Escherichia coli (strain K12)
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pMAL-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Express MBP-BirA fusion protein overnight at 18C. Lysis buffer: 50 mM Tris pH 8, 300 mM NaCl. Size exclusion buffer: 50 mM Tris pH 8.0, 150 mM NaCl.
Proper citation: RRID:Addgene_179694 Copy
Species: M13 phage
Genetic Insert: [SD8] gIII
Vector Backbone Description: Vector Backbone:SC101 ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35379961
Comments: [SD8] DddI-VSV-G
Proper citation: RRID:Addgene_179693 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 R1333A/R1335A
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Backbone Size:5969; Vector Backbone:p2CT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35422516
Proper citation: RRID:Addgene_179528 Copy
Species: Synthetic
Genetic Insert: Membrane-bound GFP RELEASE
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35177637
Proper citation: RRID:Addgene_179641 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 K233A/K234A/K253A/K263A
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Backbone Size:5969; Vector Backbone:p2CT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35422516
Proper citation: RRID:Addgene_179527 Copy
Genetic Insert: TetanusToxin
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7332; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179632 Copy
Species: Synthetic
Genetic Insert: cTVMVP
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35177637
Proper citation: RRID:Addgene_179663 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:9228; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179540 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35177637
Proper citation: RRID:Addgene_179650 Copy
Species: Homo sapiens
Genetic Insert: IFITM3
Vector Backbone Description: Vector Backbone:pCG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34321474
Proper citation: RRID:Addgene_179960 Copy
Species: Synthetic
Genetic Insert: SARS-CoV-1 M
Vector Backbone Description: Vector Backbone:pCG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33974846
Proper citation: RRID:Addgene_179967 Copy
Genetic Insert: tetR(B)r1.7
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: revTetR is expressed from a strong mycobacterial promoter (psmyc = puv15). Also see Pubmed ID 18059281.
Derivative of pMS2 with tetR r1.7 expressed by Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.
Proper citation: RRID:Addgene_17973 Copy
Species: Synthetic
Genetic Insert: SARS-CoV-1 Nsp16
Vector Backbone Description: Vector Backbone:pCG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33974846
Proper citation: RRID:Addgene_179965 Copy
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