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Species: Staphylococcus aureus
Genetic Insert: reprogrammed sortase A variant
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25187567
Comments: eSrtA(4S-9) has 120 fold change in substrate specificity with activity comparable to eSrtA and it prefers LPXSG substrate over wild-type LPXTG. See sortase A pentamutant (eSrtA) in pET29 for original eSrtA (Addgene plasmid# 75144).
Proper citation: RRID:Addgene_75146 Copy
Species: Staphylococcus aureus
Genetic Insert: reprogrammed sortase A variant
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25187567
Comments: eSrtA(2A-9) has 51,000 fold change in substrate specificity with activity comparable to eSrtA and it prefers LAXTG substrate over wild-type LPXTG. See sortase A pentamutant (eSrtA) in pET29 for original eSrtA (Addgene plasmid# 75144).
Proper citation: RRID:Addgene_75145 Copy
Species: Synthetic
Genetic Insert: mTagBFP-CAAX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75149 Copy
Species: Synthetic
Genetic Insert: mKOFP2-CAAX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2565; Vector Backbone:pDONR221; Vector Types:Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75155 Copy
Species: Synthetic
Genetic Insert: H2B-mCerulean3-P2A-mKOFP2-CAAX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pDONR221; Vector Types:Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75154 Copy
Species: Synthetic
Genetic Insert: mVenus-CAAX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2565; Vector Backbone:pDONR221; Vector Types:Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75153 Copy
Species: Synthetic
Genetic Insert: H2B-mCerulean3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2533; Vector Backbone:pDONR221; Vector Types:Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75156 Copy
Species: Synthetic
Genetic Insert: ss-HA-Mars1Cy-CD80(237-306)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3958; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26348895
Proper citation: RRID:Addgene_75221 Copy
Species: Synthetic
Genetic Insert: TagBFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2551; Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75342 Copy
Species: Synthetic
Genetic Insert: EGFP-p2a-Mars1Cy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3958; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26348895
Proper citation: RRID:Addgene_75223 Copy
Species: Synthetic
Genetic Insert: Mars1Cy-EGFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3958; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26348895
Proper citation: RRID:Addgene_75222 Copy
Species: Synthetic
Genetic Insert: TagRFPt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2551; Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75343 Copy
Species: Homo sapiens
Genetic Insert: H2bk
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4705; Vector Backbone:pDendra2-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24637833
Proper citation: RRID:Addgene_75283 Copy
Species: E. coli
Genetic Insert: lacI-pdt3B
Vector Backbone Description: Vector Backbone:pKDL071; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75466 Copy
Species: E. coli
Genetic Insert: pdt3
Vector Backbone Description: Vector Backbone:pWM91; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75450 Copy
Species: E. coli
Genetic Insert: gfp-pdt#2
Vector Backbone Description: Vector Backbone:pZE21; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75455 Copy
Species: E. coli
Genetic Insert: gfp-pdt#3A
Vector Backbone Description: Vector Backbone:pZE21; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75459 Copy
Species: E. coli
Genetic Insert: gfp-pdt#4
Vector Backbone Description: Vector Backbone:pZE21; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75457 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:mCherryC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27065233
Proper citation: RRID:Addgene_75368 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:mCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27065233
Proper citation: RRID:Addgene_75367 Copy
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