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Species: Homo sapiens
Genetic Insert: TRPC1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9215637
Proper citation: RRID:Addgene_24408 Copy
Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24402 Copy
Species: Homo sapiens
Genetic Insert: Beclin1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pDsRed-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24405 Copy
Species: Neurospora crassa
Genetic Insert: QS
Vector Backbone Description: Backbone Marker:Bischof et al., 2007; Backbone Size:9000; Vector Backbone:pUAST attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The QS cDNA was excised from pAC-QS with Acc65I and NotI, blunted, and ligated
into a blunted pUASTattB (Bischof et al., 2007) which had been digested with BglII and KpnI.
Proper citation: RRID:Addgene_24366 Copy
Species: Neurospora crassa
Genetic Insert: QF
Vector Backbone Description: Backbone Size:7700; Vector Backbone:pattB-hsp70; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: A newer version of this plasmid is now available - pattB-hsp70P-QF#7-hsp70T www.addgene.org/46134 (Addgene plasmid # 46134)
Proper citation: RRID:Addgene_24368 Copy
Genetic Insert: mCD8-GFP
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pQUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The FRT-Stop-FRT cassette from pUAS>Stop>mCD8-GFP was
excised by BglII/NotI digestion. The mCD8-GFP cassette was excised from pQUAST-mCD8GFP by NotI digestion. The two inserts were ligated into the BglII/NotI sites of the pQUAST vector.
Proper citation: RRID:Addgene_24361 Copy
Species: Homo sapiens
Genetic Insert: GSK3beta(S9A)
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: Addgene's sequence results have identified a duplicated mRFP1 and GSK S9A in this plasmid. The depositor has confirmed that this duplication should not impact plasmid functionality.
Proper citation: RRID:Addgene_24371 Copy
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Size:4283; Vector Backbone:pGL4.23; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: Obtained by subcloning 5 copies of GAL4-binding UAS sequence and the hsp70
minimal promoter from pUAS-GG into pGL4.23 using HindIII and NcoI.
Proper citation: RRID:Addgene_24343 Copy
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pPac5c-PL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: GAL80 cDNA was PCR amplified using primers PR511
(CCCCGGATCCCAACatggactacaacaagagatcttcgg) and PR512
(CGGTTAACGCGGCCGCttataaactataatgcgagatattgctaacg), and a lab vector, pCA-GAL80 as the
template. The PCR product was cloned using BamHI and NotI into pPAC5C-PL.
Proper citation: RRID:Addgene_24346 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Proper citation: RRID:Addgene_24327 Copy
Species: Neurospora crassa/other
Genetic Insert: 5xQUAS/GFP split with an intron (GG)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: This plasmid contains 5 copies of naturally occurring QF binding sites (each 16 bp
long, shown in capital letters, with spacer sequences in small letters):
GGGTAATCGCTTATCCtcGGATAAACAATTATCCtcacGGGTAATCGCTTATCCgctcGGGTAATCG
CTTATCCtcGGGTAATCGCTTATCCtt
This sequence was assembled from overlapping primers and cloned using XhoI and HindIII into
pBluescript containing hsp70 minimal promoter (Brand and Perrimon, 1993) and an optimized GFP
split with an intron (GG) (Zong et al., 2005)
Proper citation: RRID:Addgene_24336 Copy
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4283; Vector Backbone:pGL4.23; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The XhoI/NcoI fragment containing QUAS-Pmin was subcloned from pQUAS-GG
into pGL4.23 (Promega) to replace its minimal promoter. pGL4.23 contains synthetic firefly
luciferase gene (luc2), which has been codon optimized for high expression in mammalian cells.
Proper citation: RRID:Addgene_24337 Copy
Genetic Insert: Cre
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706417
Comments: ins-Cre_cryaa-Venus was generated by placing the Cre coding sequence downstream of 1.5 kb of the proximal insulin promoter. The cryaa-Venus cassette was inserted downstream of Cre in the reverse orientation using 0.54 kb of the cryaa promoter.
Proper citation: RRID:Addgene_24333 Copy
Species: Danio rerio
Genetic Insert: H2B
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706417
Comments: This plasmid was constructed using 1.5 kb of the hsp70l promoter. The floxed mCherry-STOP and H2b_GFP fragments were PCR amplified individually and sequentially cloned downstream of the hsp70l promoter. The mCherry-STOP cassette serves as a heat-shock induction marker prevents read-through translation of the H2B-GFP fusion protein, which is only expressed in cells that have undergone a Cre mediated excision event. cryaa-Cerulean was inserted downstream of HSB-GFP in the reverse orientation.
*H2B has D26G and V119I mutations. According to the depositor, GFP expression from this construct was always nuclear so there is no reason to suspect that the mutations affect the function (nuclear localization) of the H2B fragment.
Proper citation: RRID:Addgene_24334 Copy
Species: Other
Genetic Insert: SARS-CoV-2 Nucleocapsid (P199L)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34735219
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.05.455082v1 for bioRxv preprint.
Proper citation: RRID:Addgene_177949 Copy
Species: Other
Genetic Insert: Luc2
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34735219
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.05.455082v1 for bioRxv preprint.
Proper citation: RRID:Addgene_177941 Copy
Species: Other
Genetic Insert: Luc2
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34735219
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.05.455082v1 for bioRxv preprint.
Proper citation: RRID:Addgene_177942 Copy
Species: Homo sapiens
Genetic Insert: Yes-kinase associated protein
Vector Backbone Description: Backbone Size:4662; Vector Backbone:p2xFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12807903
Comments: YAP was cloned in my lab
Proper citation: RRID:Addgene_17791 Copy
Species: Homo sapiens
Genetic Insert: Yes-kinase associated protein
Vector Backbone Description: Backbone Size:4662; Vector Backbone:p2xFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12807903
Comments: YAP was cloned in my lab
Proper citation: RRID:Addgene_17792 Copy
Species: Other
Genetic Insert: SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33991487
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.05.455082v1 for bioRxv preprint.
Proper citation: RRID:Addgene_177961 Copy
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