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Plasmids are provided by Addgene and DGRC.

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On page 417 showing 8321 ~ 8340 out of 32,424 results
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  • RRID:Addgene_24408

    This resource has 1+ mentions.

http://www.addgene.org/24408

Species: Homo sapiens
Genetic Insert: TRPC1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9215637

Proper citation: RRID:Addgene_24408 Copy   


  • RRID:Addgene_24402

    This resource has 1+ mentions.

http://www.addgene.org/24402

Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19050071

Proper citation: RRID:Addgene_24402 Copy   


  • RRID:Addgene_24405

    This resource has 1+ mentions.

http://www.addgene.org/24405

Species: Homo sapiens
Genetic Insert: Beclin1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pDsRed-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19050071

Proper citation: RRID:Addgene_24405 Copy   


  • RRID:Addgene_24366

    This resource has 1+ mentions.

http://www.addgene.org/24366

Species: Neurospora crassa
Genetic Insert: QS
Vector Backbone Description: Backbone Marker:Bischof et al., 2007; Backbone Size:9000; Vector Backbone:pUAST attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The QS cDNA was excised from pAC-QS with Acc65I and NotI, blunted, and ligated into a blunted pUASTattB (Bischof et al., 2007) which had been digested with BglII and KpnI.

Proper citation: RRID:Addgene_24366 Copy   


  • RRID:Addgene_24368

    This resource has 1+ mentions.

http://www.addgene.org/24368

Species: Neurospora crassa
Genetic Insert: QF
Vector Backbone Description: Backbone Size:7700; Vector Backbone:pattB-hsp70; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: A newer version of this plasmid is now available - pattB-hsp70P-QF#7-hsp70T www.addgene.org/46134 (Addgene plasmid # 46134)

Proper citation: RRID:Addgene_24368 Copy   


  • RRID:Addgene_24361

    This resource has 1+ mentions.

http://www.addgene.org/24361

Genetic Insert: mCD8-GFP
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pQUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The FRT-Stop-FRT cassette from pUAS>Stop>mCD8-GFP was excised by BglII/NotI digestion. The mCD8-GFP cassette was excised from pQUAST-mCD8GFP by NotI digestion. The two inserts were ligated into the BglII/NotI sites of the pQUAST vector.

Proper citation: RRID:Addgene_24361 Copy   


  • RRID:Addgene_24371

    This resource has 1+ mentions.

http://www.addgene.org/24371

Species: Homo sapiens
Genetic Insert: GSK3beta(S9A)
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: Addgene's sequence results have identified a duplicated mRFP1 and GSK S9A in this plasmid. The depositor has confirmed that this duplication should not impact plasmid functionality.

Proper citation: RRID:Addgene_24371 Copy   


  • RRID:Addgene_24343

    This resource has 10+ mentions.

http://www.addgene.org/24343

Genetic Insert: Luciferase
Vector Backbone Description: Backbone Size:4283; Vector Backbone:pGL4.23; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: Obtained by subcloning 5 copies of GAL4-binding UAS sequence and the hsp70 minimal promoter from pUAS-GG into pGL4.23 using HindIII and NcoI.

Proper citation: RRID:Addgene_24343 Copy   


  • RRID:Addgene_24346

    This resource has 1+ mentions.

http://www.addgene.org/24346

Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pPac5c-PL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: GAL80 cDNA was PCR amplified using primers PR511 (CCCCGGATCCCAACatggactacaacaagagatcttcgg) and PR512 (CGGTTAACGCGGCCGCttataaactataatgcgagatattgctaacg), and a lab vector, pCA-GAL80 as the template. The PCR product was cloned using BamHI and NotI into pPAC5C-PL.

Proper citation: RRID:Addgene_24346 Copy   


  • RRID:Addgene_24327

    This resource has 1+ mentions.

http://www.addgene.org/24327

Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791

Proper citation: RRID:Addgene_24327 Copy   


  • RRID:Addgene_24336

    This resource has 1+ mentions.

http://www.addgene.org/24336

Species: Neurospora crassa/other
Genetic Insert: 5xQUAS/GFP split with an intron (GG)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: This plasmid contains 5 copies of naturally occurring QF binding sites (each 16 bp long, shown in capital letters, with spacer sequences in small letters): GGGTAATCGCTTATCCtcGGATAAACAATTATCCtcacGGGTAATCGCTTATCCgctcGGGTAATCG CTTATCCtcGGGTAATCGCTTATCCtt This sequence was assembled from overlapping primers and cloned using XhoI and HindIII into pBluescript containing hsp70 minimal promoter (Brand and Perrimon, 1993) and an optimized GFP split with an intron (GG) (Zong et al., 2005)

Proper citation: RRID:Addgene_24336 Copy   


  • RRID:Addgene_24337

    This resource has 1+ mentions.

http://www.addgene.org/24337

Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4283; Vector Backbone:pGL4.23; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The XhoI/NcoI fragment containing QUAS-Pmin was subcloned from pQUAS-GG into pGL4.23 (Promega) to replace its minimal promoter. pGL4.23 contains synthetic firefly luciferase gene (luc2), which has been codon optimized for high expression in mammalian cells.

Proper citation: RRID:Addgene_24337 Copy   


  • RRID:Addgene_24333

    This resource has 1+ mentions.

http://www.addgene.org/24333

Genetic Insert: Cre
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706417
Comments: ins-Cre_cryaa-Venus was generated by placing the Cre coding sequence downstream of 1.5 kb of the proximal insulin promoter. The cryaa-Venus cassette was inserted downstream of Cre in the reverse orientation using 0.54 kb of the cryaa promoter.

Proper citation: RRID:Addgene_24333 Copy   


http://www.addgene.org/24334

Species: Danio rerio
Genetic Insert: H2B
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706417
Comments: This plasmid was constructed using 1.5 kb of the hsp70l promoter. The floxed mCherry-STOP and H2b_GFP fragments were PCR amplified individually and sequentially cloned downstream of the hsp70l promoter. The mCherry-STOP cassette serves as a heat-shock induction marker prevents read-through translation of the H2B-GFP fusion protein, which is only expressed in cells that have undergone a Cre mediated excision event. cryaa-Cerulean was inserted downstream of HSB-GFP in the reverse orientation. *H2B has D26G and V119I mutations. According to the depositor, GFP expression from this construct was always nuclear so there is no reason to suspect that the mutations affect the function (nuclear localization) of the H2B fragment.

Proper citation: RRID:Addgene_24334 Copy   


  • RRID:Addgene_177949

    This resource has 1+ mentions.

http://www.addgene.org/177949

Species: Other
Genetic Insert: SARS-CoV-2 Nucleocapsid (P199L)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34735219
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.05.455082v1 for bioRxv preprint.

Proper citation: RRID:Addgene_177949 Copy   


  • RRID:Addgene_177941

    This resource has 1+ mentions.

http://www.addgene.org/177941

Species: Other
Genetic Insert: Luc2
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34735219
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.05.455082v1 for bioRxv preprint.

Proper citation: RRID:Addgene_177941 Copy   


  • RRID:Addgene_177942

    This resource has 1+ mentions.

http://www.addgene.org/177942

Species: Other
Genetic Insert: Luc2
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34735219
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.05.455082v1 for bioRxv preprint.

Proper citation: RRID:Addgene_177942 Copy   


  • RRID:Addgene_17791

    This resource has 10+ mentions.

http://www.addgene.org/17791

Species: Homo sapiens
Genetic Insert: Yes-kinase associated protein
Vector Backbone Description: Backbone Size:4662; Vector Backbone:p2xFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12807903
Comments: YAP was cloned in my lab

Proper citation: RRID:Addgene_17791 Copy   


  • RRID:Addgene_17792

    This resource has 1+ mentions.

http://www.addgene.org/17792

Species: Homo sapiens
Genetic Insert: Yes-kinase associated protein
Vector Backbone Description: Backbone Size:4662; Vector Backbone:p2xFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12807903
Comments: YAP was cloned in my lab

Proper citation: RRID:Addgene_17792 Copy   


  • RRID:Addgene_177961

    This resource has 1+ mentions.

http://www.addgene.org/177961

Species: Other
Genetic Insert: SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33991487
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.05.455082v1 for bioRxv preprint.

Proper citation: RRID:Addgene_177961 Copy   



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