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Species: Synthetic
Genetic Insert: SpRY
Vector Backbone Description: Vector Backbone:pRDA_085; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179094 Copy
Species: Homo sapiens
Genetic Insert: shFEN
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18394896
Comments: Target sequence: TCACTAAGCAGCACAATGAT
Proper citation: RRID:Addgene_17921 Copy
Species: Synthetic
Genetic Insert: BE3.9 (Cas9-NG)
Vector Backbone Description: Vector Backbone:pRDA_256; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179095 Copy
Species: Synthetic
Genetic Insert: ABE8e (Cas9-NG)
Vector Backbone Description: Vector Backbone:pRDA_426; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179098 Copy
Species: Mus musculus
Genetic Insert: spermatogenesis associated 33
Vector Backbone Description: Backbone Size:5211; Vector Backbone:pCAG1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34446558
Proper citation: RRID:Addgene_179131 Copy
Species: Synthetic
Genetic Insert: ABE8e (SpG)
Vector Backbone Description: Vector Backbone:pRDA_426; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179099 Copy
Species: Mus musculus
Genetic Insert: spermatogenesis associated 33
Vector Backbone Description: Backbone Size:5211; Vector Backbone:pCAG1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34446558
Proper citation: RRID:Addgene_179132 Copy
Species: Homo sapiens
Genetic Insert: WWP1 F792D
Vector Backbone Description: Backbone Marker:GEHealthcare; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16C
Proper citation: RRID:Addgene_179328 Copy
Species: Homo sapiens
Genetic Insert: H2B-mIFP-T2A-rTetR-HDAC4
Vector Backbone Description: Vector Backbone:pPB (PiggyBac); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35678392
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.04.467237v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_179440 Copy
Species: Homo sapiens
Genetic Insert: H2B-mIFP-T2A-rTetR-KRAB
Vector Backbone Description: Vector Backbone:pPB (PiggyBac); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35678392
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.04.467237v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_179439 Copy
Species: Homo sapiens
Genetic Insert: ELMSAN1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5367; Vector Backbone:pET28C (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34912035
Comments: The insert encodes the epitope (ELMSAN1 residue 550-600) of the antibody (Catalog # A303-157A, BETHYL). Expressed protein has been confirmed by Western blot.
Proper citation: RRID:Addgene_179391 Copy
Vector Backbone Description: Backbone Marker:Takara; Backbone Size:7304; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32984337
Proper citation: RRID:Addgene_179271 Copy
Species: Clostridium cellulosi
Genetic Insert: cellodextrin phosphorylase
Vector Backbone Description: Backbone Size:3464; Vector Backbone:self assembled; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35397553
Proper citation: RRID:Addgene_179276 Copy
Species: Clostridium cellulosi
Genetic Insert: cellodextrin phosphorylase
Vector Backbone Description: Backbone Size:3548; Vector Backbone:self assembled; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35397553
Proper citation: RRID:Addgene_179274 Copy
Species: A. victoria
Genetic Insert: Ptet gRNA with sfGFP dropout
Vector Backbone Description: Vector Backbone:pMC_V*_01_LVL0* Entry sfGFP (Marburg Collection); Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35338236
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.02.454823v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_179340 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting human PER2
Vector Backbone Description: Backbone Marker:Zhang lab, addgene #52961; Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34145278
Comments: Designed to be used with Addgene #179448-179452
Proper citation: RRID:Addgene_179456 Copy
Species: A. victoria
Genetic Insert: Ptet gRNA with sfGFP dropout
Vector Backbone Description: Vector Backbone:pMC_V*_01_LVL0* Entry sfGFP (Marburg Collection); Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35338236
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.02.454823v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_179336 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting human PER2
Vector Backbone Description: Backbone Marker:Zhang lab, addgene #52961; Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34145278
Comments: Designed to be used with Addgene #179448-179452
Proper citation: RRID:Addgene_179454 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting human PER2
Vector Backbone Description: Backbone Marker:Zhang lab, addgene #52961; Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34145278
Comments: Designed to be used with Addgene #179448-179452
Proper citation: RRID:Addgene_179455 Copy
Species: Homo sapiens
Genetic Insert: G0S2
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:5676; Vector Backbone:pMAL-c5E; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34260600
Comments: Protein sequence is the mature maltose binding protein (identical to aa 1-367 of accession no. 6K7E_A) + NSSSNNNNNNNNNNLG + enterokinase cleavage site (DDDDK*; * = cleavage site) + VPH + human G0S2 (G0/G1 switch protein 2; accession number NP_056529.1) + L.
Confers ampicillin-resistance.
This plasmid has been referred to as MBP g0s2, MBP-g0s2, g0s2-noHis_pMAL-c5E, or pHis6-MBP-g0s2. There is no His-tag in the expressed protein sequence.
Backbone vector is pMAL-c5E.
Construct design by James Zook.
Publication: Moran et al 2021 PLoS One 16, e0249164, https://doi.org/10.1371/journal.pone.0249164
Proper citation: RRID:Addgene_179294 Copy
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