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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pDECKO_mCherry_Intergenic Resource Report Resource Website |
RRID:Addgene_140100 | gRNA1+scaffold+H1promoter+gRNA2 | Homo sapiens | Ampicillin | PMID:35619054 | Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:04 | 0 | ||
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Salsa6f Resource Report Resource Website 1+ mentions |
RRID:Addgene_140188 | Salsa6f | Synthetic | Kanamycin | PMID:29239725 | The fusion protein in this plasmid is the fluorescent genetically encoded calcium indicator Salsa6f, which is a fusion of the dimeric red fluorescent protein tdTomato, the epitope tag V5, and the green fluorescent genetically encoded calcium indicator GCaMP6f, in order from N- to C-termini. The presence of tdTomato enables visualization of transfected cells in the absence of Ca2+ signaling and concentration-independent, ratiometric readout of Ca2+ signals. Salsa6f has been introduced into transgenic mice, where its expression is under Cre recombinase control (see PMID: 29239725). | Backbone Marker:Clontech; Backbone Size:3923; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-01 09:20:06 | 1 | |
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pDECKO_mCherry_CD19.4 Resource Report Resource Website |
RRID:Addgene_140101 | gRNA1+scaffold+H1promoter+gRNA2 | Homo sapiens | Ampicillin | PMID:35619054 | Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:04 | 0 | ||
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pDECKO_mCherry_CEACAM1 Resource Report Resource Website |
RRID:Addgene_140102 | gRNA1+scaffold+H1promoter+gRNA2 | Homo sapiens | Ampicillin | PMID:35619054 | Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:04 | 0 | ||
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pDECKO_mCherry_FURIN Resource Report Resource Website |
RRID:Addgene_140103 | gRNA1+scaffold+H1promoter+gRNA2 | Homo sapiens | Ampicillin | PMID:35619054 | Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:04 | 0 | ||
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pEU3-NII-GLICNot-C-BIOT Resource Report Resource Website |
RRID:Addgene_140186 | Ampicillin | PMID:32169064 | Backbone Size:4483; Vector Backbone:pEU3-NII-GLICNot-C-BIOT; Vector Types:expression vector for a cell-free system; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:06 | 0 | ||||
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pEU3-NII-GLICNot-C-His Resource Report Resource Website |
RRID:Addgene_140187 | Ampicillin | PMID:32169064 | Backbone Size:4456; Vector Backbone:pEU3-NII-GLICNot-C-His; Vector Types:expression vector for a cell-free system; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:06 | 0 | ||||
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pEU3-NII-GLICNot Resource Report Resource Website |
RRID:Addgene_140182 | Ampicillin | PMID:32169064 | Backbone Size:4444; Vector Backbone:pEU3-NII-GLICNot; Vector Types:expression vector for a cell-free system; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:05 | 0 | ||||
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pEU3-NII-HxHLICNot Resource Report Resource Website |
RRID:Addgene_140183 | Ampicillin | PMID:32169064 | Backbone Size:3865; Vector Backbone:pEU3-NII-HxHLICNot; Vector Types:expression vector for a cell-free system; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:05 | 0 | ||||
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pDECKO_mCherry_MIR3945HG Resource Report Resource Website |
RRID:Addgene_140108 | gRNA1+scaffold+H1promoter+gRNA2 | Homo sapiens | Ampicillin | PMID:35619054 | Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:04 | 0 | ||
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pDECKO_mCherry_KLF4 Resource Report Resource Website |
RRID:Addgene_140104 | gRNA1+scaffold+H1promoter+gRNA2 | Homo sapiens | Ampicillin | PMID:35619054 | Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:04 | 0 | ||
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pDECKO_mCherry_TREML2 Resource Report Resource Website |
RRID:Addgene_140106 | gRNA1+scaffold+H1promoter+gRNA2 | Homo sapiens | Ampicillin | PMID:35619054 | Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:04 | 0 | ||
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pDECKO_mCherry_LINC02432 Resource Report Resource Website |
RRID:Addgene_140107 | gRNA1+scaffold+H1promoter+gRNA2 | Homo sapiens | Ampicillin | PMID:35619054 | Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:04 | 0 | ||
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mVenus-hCOL1A1 Resource Report Resource Website |
RRID:Addgene_140111 | Type I procollagen α1 | Homo sapiens | Ampicillin | These human type I procollagen plasmids were designed and validated for human osteoblasts and fibroblasts similar to their previously published/shared murine counterparts e.g. eGFP-Proα1 (Plasmid #119843) https://www.addgene.org/Sergey_Leikin/. Their expression in other cells might disrupt assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in human osteoblasts and fibroblasts, overexpression of exogenous proα2 might cause intracellular trafficking and degradation of some transfected chains as monomers without integration into normal heterotrimeric (proα1)2proα2 procollagen molecules,. Overexpression of exogenous proα1 might result in excessive formation of homotrimeric (proα1)3 molecules, some of which might contain fluorescent tags on two or even all three proα1 chains, potentially causing severe disruptions in cellular function. In our experience with the murine plasmids expressed in primary osteoblasts or the MC3T3 osteoblastic cell line, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12-24 h after transfection. These were harder to observe in human Saos2 osteoblast cell line transfected with the human type I procollagen plasmids (likely due to slower fibrillogenesis and more complete cleavage of the fluorescently-tagged N-propeptide prior to collagen fibril formation). We strongly recommend following these guidelines when using the plasmids 1) Only human cells that have high expression of endogenous type I procollagen and that express fewer transfected than endogenous chains are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Stable transfection causes excessive accumulation of tagged chains in the ER over time, disruption of ER homeostasis, and disruptions and changes in cellular function. Therefore, low to moderate transient transfection is recommended for studies of physiologically relevant processes. Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid the excessive accumulation of tagged chains in the ER and cell malfunction caused by increased procollagen synthesis. 5) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | COL1A1 exons 2-3 replaced by fluorescent tag | 2026-09-01 09:20:04 | 0 | |
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Anti-GIT2 [N83/48R] Resource Report Resource Website |
RRID:Addgene_140079 | anti-GIT2 (Human) recombinant mouse monoclonal antibody | Mus musculus | Ampicillin | PMID:30667360 | RRID: AB_2750816. Isotype: IgG2a. Additional Species: human, mouse, rat. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. | Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:04 | 0 | |
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mCerulean-hCOL1A1 Resource Report Resource Website |
RRID:Addgene_140112 | Type I procollagen α1 | Homo sapiens | Ampicillin | These human type I procollagen plasmids were designed and validated for human osteoblasts and fibroblasts similar to their previously published/shared murine counterparts e.g. eGFP-Proα1 (Plasmid #119843) https://www.addgene.org/Sergey_Leikin/. Their expression in other cells might disrupt assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in human osteoblasts and fibroblasts, overexpression of exogenous proα2 might cause intracellular trafficking and degradation of some transfected chains as monomers without integration into normal heterotrimeric (proα1)2proα2 procollagen molecules,. Overexpression of exogenous proα1 might result in excessive formation of homotrimeric (proα1)3 molecules, some of which might contain fluorescent tags on two or even all three proα1 chains, potentially causing severe disruptions in cellular function. In our experience with the murine plasmids expressed in primary osteoblasts or the MC3T3 osteoblastic cell line, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12-24 h after transfection. These were harder to observe in human Saos2 osteoblast cell line transfected with the human type I procollagen plasmids (likely due to slower fibrillogenesis and more complete cleavage of the fluorescently-tagged N-propeptide prior to collagen fibril formation). We strongly recommend following these guidelines when using the plasmids 1) Only human cells that have high expression of endogenous type I procollagen and that express fewer transfected than endogenous chains are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Stable transfection causes excessive accumulation of tagged chains in the ER over time, disruption of ER homeostasis, and disruptions and changes in cellular function. Therefore, low to moderate transient transfection is recommended for studies of physiologically relevant processes. Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid the excessive accumulation of tagged chains in the ER and cell malfunction caused by increased procollagen synthesis. 5) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | COL1A1 exons 2-3 replaced by fluorescent tag | 2026-09-01 09:20:04 | 0 | |
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pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS Resource Report Resource Website |
RRID:Addgene_140196 | dSpCas9-VPR | Synthetic | Ampicillin | PMID:32526136 | To be linerised with NotI for homologous recombination in Aspergillus nidulans nkuA deficient strains, selecting with glufosinate/Basta. It can also be used for random genome integration in other fungal strains. | Backbone Size:3033; Vector Backbone:pGem; Vector Types:CRISPR, Synthetic Biology, Fungal genome integration.; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:06 | 0 | |
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pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC Resource Report Resource Website |
RRID:Addgene_140197 | dLbCas12a-VPR | Synthetic | Ampicillin and Kanamycin | PMID:32526136 | Backbone Marker:DOI: 10.1039/C8SC02870B; Backbone Size:14777; Vector Backbone:pYFAC-riboB; Vector Types:CRISPR, Synthetic Biology, Expression in Aspergillus nidulans and related filamentous fungi.; Bacterial Resistance:Ampicillin and Kanamycin | D832A DNase deactivated | 2026-09-01 09:20:06 | 0 | |
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pLenti-DENV2A-GFP-puro Resource Report Resource Website |
RRID:Addgene_140089 | DENV2A-GFP | Other | Ampicillin | PMID:31919100 | The DENV2A-GFP reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-DENV2A-GFP-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon DENV-2 infection. | Backbone Marker:Invitrogen modified by Ie-Ming Shih; Backbone Size:7057; Vector Backbone:pLenti-puro (Addgene Plasmid #39481); Vector Types:Mammalian Expression, Lentiviral, Low expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:04 | 0 | |
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pET30a-His6-LOXCATmut Resource Report Resource Website |
RRID:Addgene_140085 | A fusion of lactate oxidase from A. viridans and catalase from E. coli | Lactate oxidase from A. viridans and Catalase from E. coli | Kanamycin | PMID:31932725 | Backbone Size:8838; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Mutation in H265A and R268A in LOX (used original numbering for LOX) | 2026-09-01 09:20:04 | 0 |
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