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Genetic Insert: VSV G protein
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: There are some small discrepancies between Addgene's quality control sequence and the sequence provided by the depositor. These discrepancies should not affect the plasmid function.
Proper citation: RRID:Addgene_35616 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_35615 Copy
Species: Mus musculus
Genetic Insert: Tie2 promoter-1st exon-1st intron
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK (+); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9096345
Comments: TIE2 genomic subclone DNA containing the 10kb enhancer fragment (NaeI-SalI or NotI)
The depositing laboratory recommends digesting plasmid DNA obtained by minipreps with NaeI+SalI or NotI to verify the presence of the 10 kb enhancer fragment.
Proper citation: RRID:Addgene_35966 Copy
Species: Mus musculus
Genetic Insert: Tie2 promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescriptII SK (+); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9096345
Comments: Positive control DNA where LacZ is expressed under the control of Tie2 promoter and minimum enhancer fragment.
This plasmid is unstable in bacteria. The depositing laboratory recommends performing minipreps, as larger scale preps tend to result in recombination. To verify the plasmid, perform separate digests with KpnI and HindIII. KpnI should produce two bands at approximately 7kb and 3kb. HindIII should produce two bands at approximately 8kb and 2kb. See Addgene's diagnostic digest as an example.
Proper citation: RRID:Addgene_35964 Copy
Species: Mus musculus
Genetic Insert: Tie2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2462; Vector Backbone:pSP72; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9096345
Comments: Same as pSPTg.T2FpAXK except that there is no sv40 polyA signal fragment
Proper citation: RRID:Addgene_35963 Copy
Species: Homo sapiens
Genetic Insert: LYN
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22101434
Proper citation: RRID:Addgene_35959 Copy
Species: Homo sapiens
Genetic Insert: LYN
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22101434
Proper citation: RRID:Addgene_35958 Copy
Species: Danio rerio
Genetic Insert: humanized zebrafish lyn
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22101434
Proper citation: RRID:Addgene_35957 Copy
Species: Danio rerio
Genetic Insert: humanized zebrafish lyn
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22101434
Proper citation: RRID:Addgene_35956 Copy
Species: Mus musculus
Genetic Insert: Synectin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5250; Vector Backbone:pcDNA4/HisMax; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16908842
Comments: This construct contains a K111R mutation when compared to GenBank ID NP_061241.1 This mutation is most likely a sequence conflict, one of several (see Uniprot entry Q9Z0G0). The plasmid is exactly as described in the associated publication.
Proper citation: RRID:Addgene_35943 Copy
Species: Homo sapiens
Genetic Insert: Wnt16
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35942 Copy
Species: Homo sapiens
Genetic Insert: Wnt11
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35941 Copy
Species: Arabidopsis thaliana
Genetic Insert: 4-coumaroyl CoA ligase 1
Vector Backbone Description: Backbone Size:4449; Vector Backbone:pAC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:16551366
Comments: Initial gene cloning described in the following:
"Exploring Recombinant Flavonoid Biosynthesis in Metabolically Engineered Escherichia coli" (2004)Kevin T. Watts, Pyung Cheon Lee Dr., Claudia Schmidt-Dannert Prof. ChemBioChem. DOI: 10.1002/cbic.200300783
Proper citation: RRID:Addgene_35947 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc/his A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21857923
Comments: Site directed mutagenesis was performed on Addgene Plasmid 17609: LRRK2-WT.
Proper citation: RRID:Addgene_35946 Copy
Species: Arachis hypogaea
Genetic Insert: Stilbene synthase
Vector Backbone Description: Backbone Size:2428; Vector Backbone:pUCmod; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16551366
Proper citation: RRID:Addgene_35949 Copy
Species: Homo sapiens
Genetic Insert: Wnt6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35895 Copy
Species: Homo sapiens
Genetic Insert: Wnt5B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35894 Copy
Species: Homo sapiens
Genetic Insert: Wnt5A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35893 Copy
Species: Homo sapiens
Genetic Insert: Wnt7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35933 Copy
Species: Homo sapiens
Genetic Insert: Wnt8B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35899 Copy
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