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Species: Synthetic
Genetic Insert: Sb#15
Vector Backbone Description: Vector Backbone:pSB_init; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.04.16.045419v1 for BioRxiv preprint
Proper citation: RRID:Addgene_153523 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: This plasmid contains a SARS-CoV-2 viral sequence fragment that covers basepairs 8555 to the end of the genome. Because of sequence complexities in nsp3, it is difficult to synthesize the complete nsp3 gene. This construct may be useful to produce RNA controls for assays, as well as serve as templates to make antigens.
Proper citation: RRID:Addgene_153859 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 3'-to-5' exonuclease
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI reference sequence: YP_009725309.1
Proper citation: RRID:Addgene_153930 Copy
Species: Homo sapiens
Genetic Insert: HseIF4E
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27773676
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148669 Copy
Species: Drosophila melanogaster
Genetic Insert: DmBam_141-422
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29255063
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148833 Copy
Species: Homo sapiens
Genetic Insert: Hs4EHP_52-234opt
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28698298
Comments: Geneart clone of Hs4EHP codon optimized for expression in E.coli. sequence. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148564 Copy
Species: Homo sapiens
Genetic Insert: HsNot1iso1_1351-1588
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28165457
Comments: sequence of isoform 1 given by NCBI: NM_016284.4 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148630 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp2
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725298.1
Proper citation: RRID:Addgene_149238 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 3C-like proteinase
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725301.1
Proper citation: RRID:Addgene_149249 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 RNA-dependent RNA polymerase
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725307.1 NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149261 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 N (nucleocapsid)
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009724397.2
Proper citation: RRID:Addgene_149251 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp2
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725298.1
Proper citation: RRID:Addgene_149257 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725255.1
Proper citation: RRID:Addgene_149258 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp3
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI reference sequence: YP_009725299.1
Proper citation: RRID:Addgene_149524 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp3
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI reference sequence: YP_009725299.1
Proper citation: RRID:Addgene_149526 Copy
Genetic Insert: dnaQ926 dam seqA emrR ugi CDA1 gIII
Vector Backbone Description: Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: Please note that Addgene's NGS found a 65bp insertion in the ori region as compared to the Depositor's sequence. The effect of this insertion is unknown.
Proper citation: RRID:Addgene_140446 Copy
Genetic Insert: Azurite
Vector Backbone Description: Backbone Size:6026; Vector Backbone:pBAD18-Cm; Vector Types:Bacterial Expression, Yeast/Bacteria optimized codons; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17115054
Proper citation: RRID:Addgene_14034 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725255.1
Proper citation: RRID:Addgene_145579 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 endoRNAse
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725310.1
Proper citation: RRID:Addgene_145576 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 RNA-dependent RNA polymerase
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725307.1
Proper citation: RRID:Addgene_145577 Copy
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