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Species: Rattus norvegicus
Genetic Insert: Cx26
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Proper citation: RRID:Addgene_69016 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Comments: Addition of a fluorescent protein tag to the carboxyl-terminus of Cx43 has been shown to eliminate or attenuate interactions with ZO-1 and Occludin in cell culture. Other binding interactions and channel gating properties may be affected.
Proper citation: RRID:Addgene_69023 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Vector Backbone:EGFP-C1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Comments: Note all connexins tested that have a fluorescent protein fused to the connexin amino-terminus do not form functional channels. This plasmid forms gap junction plaque structures but does not produce intercellular coupling.
Proper citation: RRID:Addgene_69020 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Comments: Note all connexins tested that have a fluorescent protein fused to the connexin amino-terminus do not form functional channels. This plasmid forms gap junction plaque structures but does not produce intercellular coupling.
Proper citation: RRID:Addgene_69025 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Marker:Fuhlbrigge, et al 1998, PMID 3261013; Vector Backbone:pSFFV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7586229
Comments: This plasmid was provided by Joseph Stains (University of Maryland School of Medicine), who received it from Thomas Steinberg (Washington University).
Proper citation: RRID:Addgene_69442 Copy
Species: Rattus norvegicus
Genetic Insert: paGFP-Omp25
Vector Backbone Description: Backbone Marker:Jason Moffat; Backbone Size:7327; Vector Backbone:pLJM2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25837514
Proper citation: RRID:Addgene_69598 Copy
Species: Rattus norvegicus
Genetic Insert: Chimeric Synaptophysin-mCherry-Luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24529383
Proper citation: RRID:Addgene_51819 Copy
Species: Rattus norvegicus
Genetic Insert: MIBP1
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22294689
Proper citation: RRID:Addgene_51870 Copy
Species: Rattus norvegicus
Genetic Insert: MIBP1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11872163
Comments: Makino, R., Akiyama, K., Yasuda, J., Mashiyama, S., Honda, S., Sekiya, T., & Hayashi, K. (1994). Cloning and characterization of a c-myc intron binding protein (MIBP1). Nucleic acids research, 22(25), 5679-5685.
Proper citation: RRID:Addgene_51872 Copy
Species: Rattus norvegicus
Genetic Insert: CLC3
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11997263
Comments: Insert represents a portion of the 5’UTR and the full open reading frame of ClC-3 beginning at nucleotide 251 according to GenBank Accession number D17521.
Proper citation: RRID:Addgene_52423 Copy
Species: Rattus norvegicus
Genetic Insert: CLC3
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15057754
Comments: Insert represents a portion of the 5’UTR and the full open reading frame of ClC-3 beginning at nucleotide 251 according to GenBank Accession number D17521.
Proper citation: RRID:Addgene_52424 Copy
Species: Rattus norvegicus
Genetic Insert: GLUT4
Vector Backbone Description: Backbone Marker:Bogan lab, unpublished; Backbone Size:5783; Vector Backbone:pB; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11416153
Comments: Plasmid is also called pB-G7. We use FACS to select infected cells that express GFP. There is a short linker between GLUT4 and GFP.
Proper citation: RRID:Addgene_52872 Copy
Species: Rattus norvegicus
Genetic Insert: Kir4.1
Vector Backbone Description: Vector Backbone:pZac 2.1; Vector Types:Mammalian Expression, AAV, Adeno-Associated Virus (); Bacterial Resistance:Ampicillin
Defining Citation: PMID:24686787
Proper citation: RRID:Addgene_52874 Copy
Species: Rattus norvegicus
Genetic Insert: PSD-95
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4733; Vector Backbone:pTagRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: HindII and EcoRI were used to cut PSD-95 from PSD-95-pGW1 (received from David Bredt) and ligate it into pTagRFP-N. PSD-95 tyrosine 12 was mutated to glutamate (Y12E) by extending a complementary set of primers (PSD95Y12E-forward: 5’-GAC AAC CAA GAA AGA GCG CTA CCA AGA TGA AGA CAC G-3’, PSD95Y12E-reverse: 5'-CGT GTC TTC ATC TTG GTA GCG CTC TTT CTT GGT TGT C-3') along the whole template plasmid using Q5 high-fidelity DNA polymerase (NEB). DpnI was subsequently used to digest non-mutated, methylated template DNA prior to transformation.
Proper citation: RRID:Addgene_52672 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN4
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 493–511 from the start sequence of rat α-actinin-4 (the sense chain was 5’-gactatccaggagatgcagttcaagagactgcatctcctggatagtctttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52679 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN1
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 476–494 from the start sequence of rat α-actinin-1 (the sense chain was 5’-gatctccaacgtcaacaagttcaagagacttgttgacgttggagatctttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52676 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN2
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 224–242 from the start sequence of rat α-actinin-2 (the sense chain was 5’-tggcctgatggatcatgagttcaagagactcatgatccatcaggccatttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52677 Copy
Species: Rattus norvegicus
Genetic Insert: G protein alpha-o with constitutively activating R179C mutation
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17596375
Proper citation: RRID:Addgene_55780 Copy
Species: Rattus norvegicus
Genetic Insert: alpha-s (alpha3beta5/G226A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11927592
Proper citation: RRID:Addgene_55798 Copy
Species: Rattus norvegicus
Genetic Insert: Dynamin-1(K44A)-mRFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3950; Vector Backbone:Derived from pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15297467
Proper citation: RRID:Addgene_55795 Copy
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