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Vector Backbone Description: Backbone Marker:Mullins, E.D., Kang, S., 2001. Transformation: a tool for studying fungal pathogens of plants. Cell. Mol. Life Sci. 58, 2043–205; Backbone Size:7483; Vector Backbone:pDHt; Vector Types:fungal expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15893252
Proper citation: RRID:Addgene_63615 Copy
Species: Synthetic
Genetic Insert: Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
Vector Backbone Description: Backbone Size:1960; Vector Backbone:pUC; Vector Types:Mammalian Expression, Cre/Lox, FRT/Flpe recombination mediated insertion; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26492136
Proper citation: RRID:Addgene_63577 Copy
Species: Homo sapiens
Genetic Insert: USP7
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21981925
Comments: Please note that the USP7 gene in this plasmid contains mutations S600P, F679L and V1020A when compared to the annotated gene/protein sequences within the Genbank/Uniprot databases. This plasmid should function as described in the associated publication.
Proper citation: RRID:Addgene_63573 Copy
Species: Homo sapiens
Genetic Insert: UBA1
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15723079
Proper citation: RRID:Addgene_63571 Copy
Species: Drosophila melanogaster
Genetic Insert: Relish
Vector Backbone Description: Backbone Marker:lifetechnologies; Backbone Size:3500; Vector Backbone:pMT-V5-His; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63750 Copy
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: These vectors combine the advantage of efficient uracil excision reaction-based cloning (USER) and Cre-LoxP-mediated marker recycling system.
Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
USER cassette 1..14
T CYC1 20..190
loxP 257..316
T URA3 317..439
Degradation tag 440..490
K. l. LEU2 491..1576
P K. l. LEU2 1577..2606
loxP 2623..2656
Consensus TY3 3' 2796..2996
pUC ori 3777..4420
AmpR 4523..5381
Consensus TY3 5' 5825..6058
T ADH1 6085..6279
Proper citation: RRID:Addgene_63645 Copy
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: These vectors combine the advantage of efficient uracil excision reaction-based cloning (USER) and Cre-LoxP-mediated marker recycling system.
Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
USER cassette 1..14
T CYC1 20..209
loxP 257..316
T URA3 317..439
Degradation tag 440..490
K.l. URA3 491..1291
P K.l. URA3 1292..1790
loxP 1807..1840
Consensus TY4 3' 1995..2180
pUC ori 2978..3621
AmpR 3724..4582
Consensus TY4 5' 5063..5247
T ADH1 5292..5486
Proper citation: RRID:Addgene_63641 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. LEU2 477..1562
P K.l. LEU2 1563..2592
loxP 2609..2642
Consensus Ty1Cons1 3' 2782..2989
pUC ori 3762..4405
AmpR 4508..5366
Consensus Ty1Cons1 5' 5810..6043
T ADH1 6070..6264
P TEF1 6283..6702
GFP 6712..7428
Proper citation: RRID:Addgene_63655 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. LEU2 477..1562
P K.l LEU2 1563..2592
loxP 2609..2642
Consensus Ty1Cons2 3' 2782..2987
pUC ori 3761..4404
AmpR 4507..5365
Consensus Ty1Cons2 5' 5809..6040
T ADH1 6067..6261
P TEF1 6280..6699
GFP 6709..7425
Proper citation: RRID:Addgene_63656 Copy
Species: Homo sapiens
Genetic Insert: Mps1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7305; Vector Backbone:pRetrox; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25197064
Proper citation: RRID:Addgene_63702 Copy
Species: Other
Genetic Insert: T2A Cre
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7305; Vector Backbone:pRetrox; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25197064
Proper citation: RRID:Addgene_63704 Copy
Species: Homo sapiens
Genetic Insert: PCAF
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25269644
Comments: Addgene QC NGS identified a E452G discrepancy in the PCAF insert relative to Genbank ID: BC060823. It is unknown what, if any, impact this may have on function.
Proper citation: RRID:Addgene_63705 Copy
Species: Mus musculus
Genetic Insert: Gcn5
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25269644
Proper citation: RRID:Addgene_63707 Copy
Species: Mus musculus
Genetic Insert: HDAC3
Vector Backbone Description: Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16982804
Proper citation: RRID:Addgene_63676 Copy
Genetic Insert: sgSOD1, CAG promoter, mCherry, puro
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25658371
Proper citation: RRID:Addgene_63711 Copy
Species: Synthetic
Genetic Insert: EGFP-2A-PuroR
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26678082
Proper citation: RRID:Addgene_63672 Copy
Species: Synthetic
Genetic Insert: RFP cloning selection cassette
Vector Backbone Description: Backbone Marker:BioBricks Foundation; Backbone Size:2069; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26171760
Proper citation: RRID:Addgene_63674 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5731; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_63723 Copy
Genetic Insert: FLIPQTV3.0 sensor, 2mM affinity
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pcDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22723868
Proper citation: RRID:Addgene_63731 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:pAG414GPD; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Comments: Cas9 contains D839A and N863A mutations relative to reference sequence (WP_010922251.1). Depositor states that these mutations do not affect function.
Proper citation: RRID:Addgene_63801 Copy
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