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Species: Caenorhabditis elegans
Genetic Insert: odr-10
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_223457 Copy
Species: Caenorhabditis elegans
Genetic Insert: sax-3
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_223463 Copy
Species: Caenorhabditis elegans
Genetic Insert: GSPD-1(D60N)
Vector Backbone Description: Vector Backbone:pET303/CT-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34532700
Proper citation: RRID:Addgene_224154 Copy
Species: Caenorhabditis elegans
Genetic Insert: ser-2(prom3)
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_223465 Copy
Species: Caenorhabditis elegans
Genetic Insert: MDH-2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pBAD/HisA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_224146 Copy
Species: Caenorhabditis elegans
Genetic Insert: ADH-1
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_224149 Copy
Species: Caenorhabditis elegans
Genetic Insert: GPD-1
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_224147 Copy
Species: Caenorhabditis elegans
Genetic Insert: 12aa linker::mNeonGreen::TEV::3xFLAG::12aa linker
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2353; Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40463100
Comments: This plasmid contains four unique crRNA sites that can be used for re-editing the initial knock-in and inserting alternate cassettes. See this preprint for details: https://www.biorxiv.org/content/10.1101/2025.05.14.653803v1
Proper citation: RRID:Addgene_253923 Copy
Species: Caenorhabditis elegans
Genetic Insert: 12aa linker::mNeonGreen::TEV::AID*::3xFLAG::12aa linker
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2356; Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40463100
Proper citation: RRID:Addgene_253924 Copy
Species: Caenorhabditis elegans
Genetic Insert: cex-1 3'UTR
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41773048
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
There is a single nt discrepancy in Pgpc-1. This does not affect the plasmid's function.
The SNPs in pcex-1 do not change the expression pattern of Pcex-1. We observed pattern expression in RIM interneuron only. In addition, the SNP in worm mCherry also does not affect the expression or fluorescence of the RFP.
Proper citation: RRID:Addgene_253828 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3 daf-16::GFP
Vector Backbone Description: Backbone Size:7400; Vector Backbone:MosSci vector (Punc-119-mcs-att ); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41773048
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
There is a single nt discrepancy in Pgpc-1. This does not affect the plasmid's function.
Addgene found the following discrepancies in our result versus the depositor's reference:
2 SNPs in c. Briggsae unc-119(+); one results in premature stop codon Q157STOP
5 SNPs and 3bp indel in daf-16a
Comment from depositor: Functionally, the unc-119 Q7R gene does not affect it to rescue animals with unc-119 mutation. So it should be of no concern. The daf-16a SNPs and indel also do not appear to affect its function, expression or its ability to translocate from cytoplasm to nucleus.
Proper citation: RRID:Addgene_253820 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTurquoise::AID^NSEC^
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41557645
Comments: Please visit https://doi.org/10.1101/2025.04.11.648432 for bioRxiv preprint.
Proper citation: RRID:Addgene_237394 Copy
Species: Caenorhabditis elegans
Genetic Insert: mScarlet::AID^NSEC^
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41557645
Comments: Please visit https://doi.org/10.1101/2025.04.11.648432 for bioRxiv preprint.
Proper citation: RRID:Addgene_237392 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTurquoise^NSEC^
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41557645
Comments: Please visit https://doi.org/10.1101/2025.04.11.648432 for bioRxiv preprint.
Proper citation: RRID:Addgene_237393 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNeonGreen::AID^NSEC^
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41557645
Comments: Please visit https://doi.org/10.1101/2025.04.11.648432 for bioRxiv preprint.
Proper citation: RRID:Addgene_237386 Copy
Species: Caenorhabditis elegans
Genetic Insert: Peft3>TIR1 (ChrIV Repair Template with SEC)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41557645
Comments: Please visit https://doi.org/10.1101/2025.04.11.648432 for bioRxiv preprint.
Proper citation: RRID:Addgene_237395 Copy
Species: Caenorhabditis elegans
Genetic Insert: PGL-3(D1)
Vector Backbone Description: Vector Backbone:pMAL-c2X ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41862773
Proper citation: RRID:Addgene_250135 Copy
Species: Caenorhabditis elegans
Genetic Insert: PGL-3(D1-D2)
Vector Backbone Description: Vector Backbone:pMAL-c2X ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41862773
Proper citation: RRID:Addgene_250138 Copy
Species: Caenorhabditis elegans
Genetic Insert: PGL-3(FL)(R123E, K126E, K129E)
Vector Backbone Description: Vector Backbone:pMAL-c2X ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41862773
Proper citation: RRID:Addgene_250145 Copy
Species: Caenorhabditis elegans
Genetic Insert: PGL-3(D1-IDR-RGG)
Vector Backbone Description: Vector Backbone:pMAL-c2X ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41862773
Proper citation: RRID:Addgene_250143 Copy
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