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pJ2018
 
Resource Report
Resource Website 		RRID:Addgene_140665 dCas9, LuxR & Csy4 Spectinomycin PMID:32488086 GenBank file with original annotations can be downloaded from the "Resource Information" section. Vector Backbone:pCDF; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:43 0
pQCascade-IS2
 
Resource Report
Resource Website 		RRID:Addgene_140625 VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2 Vibrio cholera Spectinomycin PMID:32551502 An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally. Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:42 0
pQCascade-tra13
 
Resource Report
Resource Website 		RRID:Addgene_140628 VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-tra13 Vibrio cholera Spectinomycin PMID:32551502 An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally. Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:42 0
pQCascade
 
Resource Report
Resource Website 		RRID:Addgene_140622 VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry) Vibrio cholera Spectinomycin PMID:32551502 An unexpected 65 bp insertion was detected in the origin of replication, compared to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally. Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:42 0
pQCascade-IS1
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_140624 VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1 Vibrio cholera Spectinomycin PMID:32551502 An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally. Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:42 1
pBWB419
 
Resource Report
Resource Website 		RRID:Addgene_140637 Cas9 Spectinomycin PMID:32246719 Derivative of CRISPRi-ME from Peng et al., "Synthetic Genome Defenses against Selfish DNA Elements Stabilize Engineered Bacteria against Evolutionary Failure", ACS Synthetic Biology, 2019. Vector was modified to take the dCas9 to Cas9 and gRNA was substituted to no longer target the insertion sequences, but instead the pcaHG genes in ADP1. Vector Backbone:CRISPRi-ME (Jeffrey Barrick Laboratory); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:42 0
pBWB424
 
Resource Report
Resource Website 		RRID:Addgene_140638 Cas9 Spectinomycin PMID:32246719 Derivative of CRISPRi-ME from Peng et al., "Synthetic Genome Defenses against Selfish DNA Elements Stabilize Engineered Bacteria against Evolutionary Failure", ACS Synthetic Biology, 2019. Vector was modified to take the dCas9 to Cas9 and gRNA was substituted to no longer target the insertion sequences, but instead the vanAB genes in ADP1. Vector Backbone:CRISPRi-ME (Jeffrey Barrick Laboratory); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:42 0
pYPQ166-OsPE2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_141080 zCas9(H840A)-OsM-MLV-RT Spectinomycin PMID:32222487 Protein sequence of engineered M-MLV reverse transcriptase (D200N, L603W, T306K, W313F, T330P) was obtained from published work: Anzalone, A.V., Randolph, P.B., Davis, J.R., Sousa, A.A., Koblan, L.W., Levy, J.M., Chen, P.J., Wilson, C., Newby, G.A., Raguram, A. and Liu, D.R., 2019. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), pp.149-157. Vector Backbone:pYPQ165; Vector Types:Plant Expression, CRISPR, Gateway compatible zCas9(H840A)-OsM-MLV-RT; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:46 1
pPEPZ-sgRNAclone
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_141090 mCherry Synthetic Spectinomycin PMID:33120116 Backbone Size:4416; Vector Backbone:pPEPZ; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:46 4
pDONR223 SARS-CoV-2 NSP4
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_141258 NSP4 SARS-CoV-2 isolate Wuhan-Hu-1 Spectinomycin PMID:32763951 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Many synonymous changes due to codon optimization 2026-08-15 01:06:47 2
pDONR223 SARS-CoV-2 NSP5
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_141259 NSP5 SARS-CoV-2 isolate Wuhan-Hu-1 Spectinomycin PMID:32763951 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Many synonymous changes due to codon optimization 2026-08-15 01:06:47 3
pDONR223 SARS-CoV-2 NSP9
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_141263 NSP9 SARS-CoV-2 isolate Wuhan-Hu-1 Spectinomycin PMID:32763951 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Many synonymous changes due to codon optimization 2026-08-15 01:06:47 2
pDONR223 SARS-CoV-2 NSP6
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_141260 NSP6 SARS-CoV-2 isolate Wuhan-Hu-1 Spectinomycin PMID:32763951 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Many synonymous changes due to codon optimization 2026-08-15 01:06:47 3
pDONR223 SARS-CoV-2 NSP7
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_141261 NSP7 SARS-CoV-2 isolate Wuhan-Hu-1 Spectinomycin PMID:32763951 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Many synonymous changes due to codon optimization 2026-08-15 01:06:47 1
pDONR223 SARS-CoV-2 NSP14
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_141267 NSP14 SARS-CoV-2 isolate Wuhan-Hu-1 Spectinomycin PMID:32763951 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Many synonymous changes due to codon optimization 2026-08-15 01:06:47 3
pDONR223 SARS-CoV-2 ORF7B
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_141277 ORF7B SARS-CoV-2 isolate Wuhan-Hu-1 Spectinomycin PMID:32763951 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Many synonymous changes due to codon optimization 2026-08-15 01:06:47 2
pDONR223 SARS-CoV-2 ORF6
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_141275 ORF6 SARS-CoV-2 isolate Wuhan-Hu-1 Spectinomycin PMID:32763951 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Many synonymous changes due to codon optimization 2026-08-15 01:06:47 1
pDONR223 SARS-CoV-2 ORF3B
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_141272 ORF3B SARS-CoV-2 isolate Wuhan-Hu-1 Spectinomycin PMID:32763951 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Many synonymous changes due to codon optimization 2026-08-15 01:06:47 1
pZYIntExc
 
Resource Report
Resource Website 		RRID:Addgene_139673 phiC31 Excisionase bacteriophage p1 Spectinomycin PMID:32166212 Backbone Size:8838; Vector Backbone:pZY101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:35 0
pCO604
 
Resource Report
Resource Website 		RRID:Addgene_139737 H3H Neonothopanus nambi Spectinomycin PMID:32209230 Vector Backbone:pICH41308; Vector Types:Luciferase, MoClo Level 0 component; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:35 0

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