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Genetic Insert: dCas9, LuxR & Csy4
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32488086
Comments: GenBank file with original annotations can be downloaded from the "Resource Information" section.
Proper citation: RRID:Addgene_140665 Copy
Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32551502
Comments: An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140625 Copy
Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-tra13
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32551502
Comments: An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140628 Copy
Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32551502
Comments: An unexpected 65 bp insertion was detected in the origin of replication, compared to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140622 Copy
Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32551502
Comments: An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140624 Copy
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:CRISPRi-ME (Jeffrey Barrick Laboratory); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32246719
Comments: Derivative of CRISPRi-ME from Peng et al., "Synthetic Genome Defenses against Selfish DNA Elements Stabilize Engineered Bacteria against Evolutionary Failure", ACS Synthetic Biology, 2019. Vector was modified to take the dCas9 to Cas9 and gRNA was substituted to no longer target the insertion sequences, but instead the pcaHG genes in ADP1.
Proper citation: RRID:Addgene_140637 Copy
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:CRISPRi-ME (Jeffrey Barrick Laboratory); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32246719
Comments: Derivative of CRISPRi-ME from Peng et al., "Synthetic Genome Defenses against Selfish DNA Elements Stabilize Engineered Bacteria against Evolutionary Failure", ACS Synthetic Biology, 2019. Vector was modified to take the dCas9 to Cas9 and gRNA was substituted to no longer target the insertion sequences, but instead the vanAB genes in ADP1.
Proper citation: RRID:Addgene_140638 Copy
Genetic Insert: zCas9(H840A)-OsM-MLV-RT
Vector Backbone Description: Vector Backbone:pYPQ165; Vector Types:Plant Expression, CRISPR, Gateway compatible zCas9(H840A)-OsM-MLV-RT; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32222487
Comments: Protein sequence of engineered M-MLV reverse transcriptase (D200N, L603W, T306K, W313F, T330P) was obtained from published work: Anzalone, A.V., Randolph, P.B., Davis, J.R., Sousa, A.A., Koblan, L.W., Levy, J.M., Chen, P.J., Wilson, C., Newby, G.A., Raguram, A. and Liu, D.R., 2019. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), pp.149-157.
Proper citation: RRID:Addgene_141080 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:4416; Vector Backbone:pPEPZ; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33120116
Proper citation: RRID:Addgene_141090 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP4
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141258 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP5
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141259 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP9
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141263 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP6
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141260 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP7
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141261 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP14
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141267 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF7B
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141277 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF6
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141275 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF3B
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141272 Copy
Species: bacteriophage p1
Genetic Insert: phiC31 Excisionase
Vector Backbone Description: Backbone Size:8838; Vector Backbone:pZY101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32166212
Proper citation: RRID:Addgene_139673 Copy
Species: Neonothopanus nambi
Genetic Insert: H3H
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Luciferase, MoClo Level 0 component; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32209230
Proper citation: RRID:Addgene_139737 Copy
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