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Species: Homo sapiens
Genetic Insert: EGFR ECD (1-644)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17283074
Proper citation: RRID:Addgene_42666 Copy
Species: Homo sapiens
Genetic Insert: EGFR ICD (645-1186)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17283074
Proper citation: RRID:Addgene_42667 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16224021
Comments: Please note that there is an additional mutation in EZH2 ORF, Q3R. The mutation does not affect plasmid function.
Proper citation: RRID:Addgene_42664 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17283074
Proper citation: RRID:Addgene_42665 Copy
Species: Homo sapiens
Genetic Insert: NKX3.1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10871372
Comments: This is a full length NKX3.1 cDNA construct.
Proper citation: RRID:Addgene_42897 Copy
Species: Homo sapiens
Genetic Insert: protein kinase R
Vector Backbone Description: Vector Backbone:pet11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15567412
Proper citation: RRID:Addgene_42934 Copy
Species: Homo sapiens
Genetic Insert: NKX3.1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET-30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19780584
Comments: This is a full length NKX3.1 cDNA construct. Protein expressed in E.coli BL21(DE3) competent cells
Proper citation: RRID:Addgene_42896 Copy
Species: Homo sapiens
Genetic Insert: PECAM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_42902 Copy
Species: Homo sapiens
Genetic Insert: Cry2PHR-mCherry-LRP6c
Vector Backbone Description: Backbone Size:7788; Vector Backbone:CLPIT; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23377377
Proper citation: RRID:Addgene_42961 Copy
Species: Homo sapiens
Genetic Insert: Cry2PHR-mCherry-RhoA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3991; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23377377
Proper citation: RRID:Addgene_42958 Copy
Species: Homo sapiens
Genetic Insert: Cry2PHR-mCherry-RhoA
Vector Backbone Description: Backbone Size:7821; Vector Backbone:CLPIT; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23377377
Proper citation: RRID:Addgene_42959 Copy
Species: Homo sapiens
Genetic Insert: Negative control Functionally Dead Human L1 element
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16507671
Comments: These plasmids consist of the L1RP element tagged with an enhanced green fluorescent protein (EGFP) cassette (pL1RP-EGFP) in a pCEP4 backbone (Invitrogen). The L1 element is driven by its 5′-UTR and an upstream CMV MIE promoter. L1-EGFP (EF06R, Addgene plasmid #42940) was derived from L1RP-EGFP by blunt ligation of 1–1392 nt of pPur (BD/Clontech) into the NruI site of pCEP4.
A negative control ‘dead’ L1-EGFP (EF05J, Addgene plasmid #42941) was similarly derived from pL1RP(JM111)-EGFP, which contains disabling mutations in ORF1.
An EN− plasmid (EF13E, Addgene plasmid #42942) was created by swapping 1927–3708 nt (Age I–Bcl I) from JM102D205A, which contains an endonuclease function-abrogating D205A point mutation in the EN domain, into EF06R.
A control plasmid (EF12J, Addgene plasmid #42943) was similarly derived from JM102. JM102 and JM102D205A are derived from L1.3, which differs from L1RP by 10 base changes in the region swapped.
The A250V mutation found during Addgene QC is conservative and should not affect function.
Proper citation: RRID:Addgene_42941 Copy
Species: Homo sapiens
Genetic Insert: Human L1 element
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16507671
Comments: These plasmids consist of the L1RP element tagged with an enhanced green fluorescent protein (EGFP) cassette (pL1RP-EGFP) in a pCEP4 backbone (Invitrogen). The L1 element is driven by its 5′-UTR and an upstream CMV MIE promoter. L1-EGFP (EF06R, Addgene plasmid #42940) was derived from L1RP-EGFP by blunt ligation of 1–1392 nt of pPur (BD/Clontech) into the NruI site of pCEP4.
A negative control ‘dead’ L1-EGFP (EF05J, Addgene plasmid #42941) was similarly derived from pL1RP(JM111)-EGFP, which contains disabling mutations in ORF1.
An EN− plasmid (EF13E, Addgene plasmid #42942) was created by swapping 1927–3708 nt (Age I–Bcl I) from JM102D205A, which contains an endonuclease function-abrogating D205A point mutation in the EN domain, into EF06R.
A control plasmid (EF12J, Addgene plasmid #42943) was similarly derived from JM102. JM102 and JM102D205A are derived from L1.3, which differs from L1RP by 10 base changes in the region swapped.
I->T substitution at the end of ORF 2 should not affect plasmid function.
Proper citation: RRID:Addgene_42940 Copy
Species: Homo sapiens
Genetic Insert: DNAJA4 CDS
Vector Backbone Description: Backbone Size:5165; Vector Backbone:pBabe-PURO; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23142051
Proper citation: RRID:Addgene_43799 Copy
Species: Homo sapiens
Genetic Insert: LIN28B
Vector Backbone Description: Backbone Size:5372; Vector Backbone:pFRT/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23481595
Comments: Note that the Flag and HA tags are not in frame in the depositor's assembled sequence, but Addgene's quality control sequence shows they are in frame.
Proper citation: RRID:Addgene_43798 Copy
Species: Homo sapiens
Genetic Insert: WNT10B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43823 Copy
Species: Homo sapiens
Genetic Insert: human WNT11
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43824 Copy
Species: Homo sapiens
Genetic Insert: WNT8A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43818 Copy
Species: Homo sapiens
Genetic Insert: WNT8B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43819 Copy
Species: Homo sapiens
Genetic Insert: WNT4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43812 Copy
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