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Species: Homo sapiens
Genetic Insert: MED7
Vector Backbone Description: Backbone Marker:Invitrogen, modified at Conaway lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 N-FLAG/Hyg; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12584197
Proper citation: RRID:Addgene_15416 Copy
Species: Homo sapiens
Genetic Insert: MED27
Vector Backbone Description: Backbone Marker:Invitrogen, modified at Conaway lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 N-FLAG/Hyg; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12584197
Proper citation: RRID:Addgene_15424 Copy
Species: Homo sapiens
Genetic Insert: MED22
Vector Backbone Description: Backbone Marker:Invitrogen, modified at Conaway lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 N-FLAG/Hyg; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12584197
Proper citation: RRID:Addgene_15423 Copy
Species: Homo sapiens
Genetic Insert: Sec13
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32719541
Proper citation: RRID:Addgene_154215 Copy
Species: Homo sapiens
Genetic Insert: SEH1L
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32719541
Proper citation: RRID:Addgene_154216 Copy
Species: S. sciureus (squirrel monkey)
Genetic Insert: CHMP3
Vector Backbone Description: Backbone Marker:#11154; Backbone Size:4311; Vector Backbone:pEF-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34597582
Proper citation: RRID:Addgene_154172 Copy
Species: S. sciureus (squirrel monkey)
Genetic Insert: CHMP3(155)
Vector Backbone Description: Backbone Marker:#11154; Backbone Size:4311; Vector Backbone:pEF-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34597582
Proper citation: RRID:Addgene_154173 Copy
Species: S. sciureus (squirrel monkey)
Genetic Insert: retroCHMP3
Vector Backbone Description: Backbone Marker:#11154; Backbone Size:4311; Vector Backbone:pEF-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34597582
Proper citation: RRID:Addgene_154174 Copy
Species: Synthetic
Genetic Insert: p40S:QfDBD-VP16AD-GFP-NLS
Vector Backbone Description: Backbone Marker:MoClo Tool Kit of Sylvestre Marillonnet; Backbone Size:8102; Vector Backbone:pAGM4673; Vector Types:Bacterial Expression, Synthetic Biology, Fungal Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31739777
Comments: Synthetic Transcription Factor (QfDBD-VP16AD-GFP-NLS) and DsRed-SKL transcribed with various full and core promoters (CP) with and without Quinic acid Upstream Activation Sequences (QUAS). Fungal selection provided with amdS marker. Flanking regions were designed for integration at the locus of the Penicillin gene cluster.
Proper citation: RRID:Addgene_154228 Copy
Species: Homo sapiens
Genetic Insert: Ash2L
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Comments: Full-length ASH2L cDNA was amplified by reverse transcription-PCR from HeLaS cells using primers 5'-TAT AGG ATC CTG GTG ATG GCG GCG GCA GGA G-3' and 5'-CAG CGA CTC GAG TCA GGG TTC CCA TGG GGG AC-3'.
Proper citation: RRID:Addgene_15549 Copy
Species: Homo sapiens
Genetic Insert: Ash2L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Comments: Full-length ASH2L cDNA was amplified by reverse transcription-PCR from HeLaS cells using primers 5'-TAT AGG ATC CTG GTG ATG GCG GCG GCA GGA G-3' and 5'-CAG CGA CTC GAG TCA GGG TTC CCA TGG GGG AC-3'
Please note that mutation R622H in ASH2L was found during Addgene's quality control. The plasmid should function as described.
Proper citation: RRID:Addgene_15548 Copy
Species: Homo sapiens
Genetic Insert: htt 103Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832050
Comments: Figure 2, VI in article.
Lindquist lab number: 7704
Proper citation: RRID:Addgene_15579 Copy
Species: Homo sapiens
Genetic Insert: htt 103Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832050
Comments: Figure 1C in article.
Lindquist lab number: 7720
Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.
Proper citation: RRID:Addgene_15587 Copy
Species: Homo sapiens
Genetic Insert: htt 25Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832050
Comments: Figure 1C in article.
Lindquist lab number: 7719
Proper citation: RRID:Addgene_15584 Copy
Species: Homo sapiens
Genetic Insert: YTHDF1
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pSNAPf; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32451507
Proper citation: RRID:Addgene_155346 Copy
Species: Homo sapiens
Genetic Insert: YTHDF2
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pSNAPf; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32451507
Proper citation: RRID:Addgene_155347 Copy
Species: Synthetic
Genetic Insert: humanized EBpaRS/BstYam
Vector Backbone Description: Backbone Marker:Clontech, major modifications; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32929862
Comments: The plasmid has been developed for efficient incorporation of Bpa in mammalian cells, first reported in Böttke et al, EMBO reports, 2020. With respect to similar plasmids from other labs, this plasmid contains a humanized gene for EBpaRS and 4 copies of the U6-BstYam tRNA cassette downstream of the synthetase cassette.
Proper citation: RRID:Addgene_155342 Copy
Vector Backbone Description: Backbone Size:6866; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.60, Ligation number L3568.
Proper citation: RRID:Addgene_1560 Copy
Species: Mus musculus
Genetic Insert: M71 Targeting Vector
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS; Vector Types:high copy number; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15186781
Proper citation: RRID:Addgene_15635 Copy
Species: Mus musculus
Genetic Insert: mouse SMC3
Vector Backbone Description: Vector Backbone:pKS037-pCAGGS-3XFLAG-Smc3-mKate2-bpAk; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33154377
Proper citation: RRID:Addgene_156447 Copy
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