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Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:4124; Vector Backbone:pTrc99A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36511859
Proper citation: RRID:Addgene_188967 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB-MeCP2
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167901 Copy
Species: Synthetic
Genetic Insert: Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
Vector Backbone Description: Backbone Marker:GeneArt (Thermo Fisher Scientific); Backbone Size:2414; Vector Backbone:pOA-RQ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36220271
Proper citation: RRID:Addgene_186030 Copy
Species: Synthetic
Genetic Insert: CalfluxCTN
Vector Backbone Description: Backbone Marker:Dr. Michael Lin and his colleagues; Backbone Size:6075; Vector Backbone:pcDNA3.1/Puro-CAG-VSFP-CR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35973028
Comments: CalfluxCTN BRET-based calcium sensor composed of the following 5 segments: Segment 1: Clover C9 - Amino acid residues 1-230 of Clover, Segment 2: Linker - MQVAD, Segment 3: TnC - EF-hand motif derived from Opsanus tau troponin C, Segment 4: Linker - IYPEL, Segment 5: NanoLuc - NanoLuc luciferase
Proper citation: RRID:Addgene_190068 Copy
Species: Synthetic
Genetic Insert: mApple
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR 221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_187690 Copy
Species: Synthetic
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167886 Copy
Species: Synthetic
Genetic Insert: NLS-CanlonicSF
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4843; Vector Backbone:pCMV-myc-nuc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36306435
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.01.322404v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_188390 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167870 Copy
Species: Synthetic
Genetic Insert: loxP-eGFP-attP-Bsd-lox251
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36313798
Proper citation: RRID:Addgene_183751 Copy
Species: Synthetic
Genetic Insert: loxP-eGFP-attP-Bsd-lox257
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36313798
Proper citation: RRID:Addgene_183754 Copy
Species: Synthetic
Genetic Insert: FRT-mCherry-attP-BleoR-F3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36313798
Proper citation: RRID:Addgene_183755 Copy
Species: Synthetic
Genetic Insert: FRT-mCherry-attP-BleoR-F3
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36313798
Proper citation: RRID:Addgene_183752 Copy
Species: Synthetic
Genetic Insert: Cas9-VPR
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167894 Copy
Species: Synthetic
Genetic Insert: 3XHA-HALO-NAT
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Comments: This constructed is intended for use as a template for PCR and not for expression of the insert.
Proper citation: RRID:Addgene_188931 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB-MeCP2
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167898 Copy
Species: Synthetic
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti6.3/V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23990882
Proper citation: RRID:Addgene_187687 Copy
Species: Synthetic
Genetic Insert: ASAP2f
Vector Backbone Description: Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36313798
Proper citation: RRID:Addgene_183760 Copy
Species: Synthetic
Genetic Insert: His8OphMA∆C6-TEV18mer
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.07.21.500988v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_189671 Copy
Species: Synthetic
Genetic Insert: attL
Vector Backbone Description: Backbone Marker:Bierman M. et al. Gene 1992 Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.; Backbone Size:3469; Vector Backbone:pOJ260; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:35456877
Proper citation: RRID:Addgene_172193 Copy
Species: Synthetic
Genetic Insert: humanized hfCas13X
Vector Backbone Description: Vector Backbone:piggyBac-CBh; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35953673
Proper citation: RRID:Addgene_190033 Copy
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