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Species: Other
Genetic Insert: FlaviA-GFP
Vector Backbone Description: Backbone Marker:Invitrogen modified by Ie-Ming Shih; Backbone Size:7057; Vector Backbone:pLenti-puro (Addgene Plasmid #39481); Vector Types:Mammalian Expression, Lentiviral, Low expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The FlaviA-GFP reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-FlaviA-GFP-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection.
Proper citation: RRID:Addgene_140088 Copy
Species: Synthetic
Genetic Insert: pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Vector Backbone Description: Backbone Marker:Joung Lab (DR274); Vector Backbone:DR274 (Addgene Plasmid #42250); Vector Types:Bacterial Expression, In vitro transcription (mRNA synthesis); Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_140082 Copy
Species: Lactate oxidase from A. viridans and catalase from E. coli
Genetic Insert: A fusion of lactate oxidase from A. viridans and catalase from E. coli
Vector Backbone Description: Backbone Size:8838; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31932725
Proper citation: RRID:Addgene_140084 Copy
Species: Sinomenium acutum
Genetic Insert: Dechloroacutumine halogenase from Sinomenium acutum
Vector Backbone Description: Backbone Size:5276; Vector Backbone:pHis8-4b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32313070
Comments: Expressed robustly in E.coli. Catalyzes chlorination of dechloroacutumine in vitro
Proper citation: RRID:Addgene_140128 Copy
Species: Menispermum canadense
Genetic Insert: Dechloroacutumine halogenase from Menispermum canadense
Vector Backbone Description: Backbone Size:5276; Vector Backbone:pHis8-4b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32313070
Comments: Expressed robustly in E.coli. Catalyzes chlorination of dechloroacutumine in vitro
Proper citation: RRID:Addgene_140129 Copy
Species: Synthetic
Genetic Insert: CDreV
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32203389
Comments: Funded by the BRAIN Initiative.
Proper citation: RRID:Addgene_140133 Copy
Species: Synthetic
Genetic Insert: iDreV
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32203389
Comments: Funded by the BRAIN Initiative.
Proper citation: RRID:Addgene_140136 Copy
Species: Mus musculus
Genetic Insert: Tcf4B
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14667415
Proper citation: RRID:Addgene_14019 Copy
Species: Synthetic
Genetic Insert: sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
Vector Backbone Description: Vector Backbone:pUCIDT; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231336
Proper citation: RRID:Addgene_140096 Copy
Species: Sinomenium acutum
Genetic Insert: Dechloroacutumine halogenase G226D variant from Sinomenium acutum
Vector Backbone Description: Backbone Size:5276; Vector Backbone:pHis8-4b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32313070
Comments: Expressed robustly in E.coli. Catalyzes hydroxylation of dechloroacutumine in vitro
Proper citation: RRID:Addgene_140130 Copy
Species: Mus musculus
Genetic Insert: Wnt1
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Comments: Contains an internal Cla I site, from Javier Capdevila.
Proper citation: RRID:Addgene_14017 Copy
Species: Synthetic
Genetic Insert: NCreV
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32203389
Comments: Funded by the BRAIN Initiative.
Proper citation: RRID:Addgene_140131 Copy
Species: Homo sapiens
Genetic Insert: gRNA1+scaffold+H1promoter+gRNA2
Vector Backbone Description: Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35619054
Proper citation: RRID:Addgene_140099 Copy
Species: Gallus gallus
Genetic Insert: Slug
Vector Backbone Description: Backbone Size:3000; Vector Backbone:Slax13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full length coding sequence, PCR (vent) product, 5’ primer converts 2nd codon from cca (pro) to gca (ala) in order to generate a Nco I site; 3’ primer is wt plus EcoRI site, from Malcolm Logan.
Proper citation: RRID:Addgene_14015 Copy
Species: Gallus gallus
Genetic Insert: HoxD10
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full-length Hox D10, from Craig Nelson.
Proper citation: RRID:Addgene_14010 Copy
Species: Other
Genetic Insert: FlaviA-mNeptune
Vector Backbone Description: Backbone Marker:Eric Campeau, Paul Kaufman; Backbone Size:7871; Vector Backbone:pLenti CMV GFP Puro (658-5) (Addgene Plasmid #17448); Vector Types:Mammalian Expression, Lentiviral, High expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The FlaviA-mNeptune reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter and/or generate OSER structures - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-CMV-FlaviA-mNeptune-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection.
Proper citation: RRID:Addgene_140091 Copy
Species: Synthetic
Genetic Insert: iFlpV
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32203389
Comments: Funded by the BRAIN Initiative.
Proper citation: RRID:Addgene_140137 Copy
Genetic Insert: lacY
Vector Backbone Description: Backbone Size:4938; Vector Backbone:pAN1818; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32355194
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_140139 Copy
Species: Synthetic
Genetic Insert: Stabilized hairpin of miR-let7d
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Comments: let7d_stbC motif was inserted between BamHI and AgeI sites of pAptamerCassette-EGFP (ID: 140288).
Proper citation: RRID:Addgene_140266 Copy
Species: Homo sapiens
Genetic Insert: LIN28A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Comments: CDS of LIN28A was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140267 Copy
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