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Species: MS2 Phage
Genetic Insert: Maturation Protein
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2200; Vector Backbone:pMX; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_180229 Copy
Species: Capsaspora owczarzaki
Genetic Insert: Co-NF-kB
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916615
Comments: Codon optimized for human cell expression
Proper citation: RRID:Addgene_180197 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike L241-L242-A243-
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180070 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 N S235F
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180071 Copy
Species: Capsaspora owczarzaki
Genetic Insert: Co-NF-kBCterm
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916615
Comments: Codon optimized for human cell expression
Proper citation: RRID:Addgene_180199 Copy
Genetic Insert: CasMINI sgRNA (backbone) and BFP
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34480847
Proper citation: RRID:Addgene_180280 Copy
Species: Mus musculus
Genetic Insert: Wnt-4HA
Vector Backbone Description: Backbone Size:6620; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.
Proper citation: RRID:Addgene_18031 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*24:02
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00050 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180445 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*08:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00146 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180447 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*01:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00001 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180448 Copy
Species: Macaca mulatta (Rhesus Macaque)
Genetic Insert: Mamu-A1*002:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET23a+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: https://www.ebi.ac.uk/ipd/mhc/allele/?accession=NHP01866 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180443 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*35:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00237 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180450 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*03:01
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEMEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00037 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180451 Copy
Species: Mus musculus
Genetic Insert: Wnt-5b
Vector Backbone Description: Backbone Size:6620; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.
Proper citation: RRID:Addgene_18034 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*02:01 (CD8 mut)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Mutation prevents binding of CD8 (Purbhoo et al. (2001) J. Biol. Chem. doi.org/10.1074/jbc.M102498200). See https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00005 for allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180459 Copy
Species: Mus musculus
Genetic Insert: WNT-7A
Vector Backbone Description: Backbone Size:6620; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.
Proper citation: RRID:Addgene_18036 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180334 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180339 Copy
Species: Homo sapiens
Genetic Insert: RPS6KA2
Vector Backbone Description: Backbone Size:8015; Vector Backbone:W118-1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33709453
Proper citation: RRID:Addgene_180384 Copy
Species: Homo sapiens
Genetic Insert: RPS6KA2_K91A/K444A
Vector Backbone Description: Backbone Size:8015; Vector Backbone:W118-1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33709453
Proper citation: RRID:Addgene_180385 Copy
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