Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Homo sapiens
Genetic Insert: Src deletion mutant
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684
Proper citation: RRID:Addgene_140301 Copy
Species: Synthetic
Genetic Insert: Two PP7CP-binding sites embedded in scaffold
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: scPP7(2xWT) motif was inserted between BamHI and AgeI sites of pAptamerCassette-EGFP (ID: 140288).
Proper citation: RRID:Addgene_140261 Copy
Species: Homo sapiens
Genetic Insert: U1A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: CDS of WT U1A was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140264 Copy
Species: Other
Genetic Insert: Coat protein of bacteriphage MS2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: CDS of mutant MS2CP (MS2CP(V29I)) was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140260 Copy
Species: Homo sapiens
Genetic Insert: Src deletion mutant
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684
Proper citation: RRID:Addgene_140306 Copy
Species: Homo sapiens
Genetic Insert: Truncated U1A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Comments: CDS of Truncated U1A was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140269 Copy
Species: Homo sapiens
Genetic Insert: Src deletion mutant
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684
Proper citation: RRID:Addgene_140303 Copy
Species: Homo sapiens
Genetic Insert: Src deletion mutant
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684
Proper citation: RRID:Addgene_140305 Copy
Species: Homo sapiens
Genetic Insert: Src deletion mutant
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684
Proper citation: RRID:Addgene_140310 Copy
Species: Empty backbone
Genetic Insert: Empty backbone
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Proper citation: RRID:Addgene_140278 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pGbait; Vector Types:Mouse Targeting; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140279 Copy
Species: Homo sapiens
Genetic Insert: Src mutant
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684
Proper citation: RRID:Addgene_140312 Copy
Species: Synthetic
Genetic Insert: BoxCD
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20016495
Comments: Alternative name: p1-boxC/D-EGFP
Proper citation: RRID:Addgene_140272 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pBluescript II SK+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140273 Copy
Species: Synthetic
Genetic Insert: mKate2
Vector Backbone Description: Vector Backbone:Derived from AAV V032; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32187527
Proper citation: RRID:Addgene_140274 Copy
Species: Mus musculus
Genetic Insert: Csrp2
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:pDTA-4; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip.
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). Thus, the sfGFP fluorescence should be detectable only in cells in which the marker gene expression level is high.
Homology arms subcloned by recombineering from a clone of RPCI-24 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Please note there are a few ambiguous bases in Addgene’s NGS result. This is due to repetitive T-A regions. Depositor confirms these bases do not affect plasmid function.
Proper citation: RRID:Addgene_140270 Copy
Species: Empty backbone
Genetic Insert: Empty backbone
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Proper citation: RRID:Addgene_140288 Copy
Vector Backbone Description: Backbone Marker:DOI: 10.1039/C8SC02870B; Backbone Size:11339; Vector Backbone:New backbone modified from pYFAC-pyroA; Vector Types:CRISPR, Synthetic Biology, Cloning of crRNA and expression in Aspergillus nidulans and related filamentous fungi.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32526136
Comments: Cloning of crRNA arrays is done with BsmbI digestion of the vector and ligation of annealed oligonucleotides containing the crRNA sequences, making use of the two crRNA scaffolds in the vector to start and finish the array. Electroporation is recommended for higher efficiency in cloning.
Proper citation: RRID:Addgene_140201 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pX330A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140285 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pX330A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140280 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within RRID that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
