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Species: Homo sapiens
Genetic Insert: human CTCF
Vector Backbone Description: Vector Backbone:pKS070-pCAGGS-3XFLAG-(human)CTCF-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33154377
Comments: This plasmid has been found to have dimerized. Plasmid dimerization often does not impact plasmid function, but may reduce transformation efficiencies. If you still need to isolate the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_156448 Copy
Species: SARS CoV2
Genetic Insert: SARS-CoV-2 S protein
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33243994
Comments: Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors
Proper citation: RRID:Addgene_156421 Copy
Species: Mus musculus
Genetic Insert: CTCF, rtta3G, TetO3G
Vector Backbone Description: Vector Backbone:pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE.; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33154377
Proper citation: RRID:Addgene_156430 Copy
Species: Homo sapiens
Genetic Insert: LAP2
Vector Backbone Description: Backbone Marker:Available at Addgene (#1764); Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16959612
Comments: Human LAP2 was cloned by PCR from cDNA template and then digested with EcoR1/SalI and inserted in pBabe-Puro.
Proper citation: RRID:Addgene_15712 Copy
Species: Mus musculus
Genetic Insert: PRAS40 shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17510057
Comments: Oligonucleotides encoding the short-hairpin RNA expression cassette targeting mouse PRAS40 from base 753-773 (GI:124376717) were annealed and subcloned into pLKO.1. See author's map for shRNA sequence information.
Proper citation: RRID:Addgene_15672 Copy
Vector Backbone Description: Backbone Size:8081; Vector Backbone:pCM433; Vector Types:bacterial allelic exchange vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18710539
Comments: Ampicillin, Chloramphenicol, Tetracycline
Proper citation: RRID:Addgene_15670 Copy
Species: Homo sapiens
Genetic Insert: TIF1 gamma shRNA
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pRetrosuper-GFP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751102
Comments: shRNA oligos were first cloned into pRetrosuper-Puro digested with BglII and HindIII.
Next, the AgeI/XhoI fragments from shRNA sequence-containing pRetrosuper-Puro plasmids were cloned into the AgeI/XhoI site of pRetrosuper-GFP.
Sense oligo: 5'-gatcccc GAAGATGTCT CAGAGTCTG ttcaagaga CAGACTCTGA GACATCTTC tttttggaaa-3'.
Proper citation: RRID:Addgene_15721 Copy
Species: Homo sapiens
Genetic Insert: YAP
Vector Backbone Description: Backbone Marker:Available at Addgene (#1764); Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16894141
Comments: The human YAP ORF was cloned into the pBABEpuro vector as an EcoRI–BamHI fragment. A single FLAG tag was added to the N terminus during PCR with the following primers: Hs.YAP.F, CCGGGATCCACCATGGATTACAAGGATGACGACGATAAGATGGACCCCGGGCAGCAGCCCGCCGC; and Hs.YAP.R, CCGGAATTCCTATAACCATGTAAGAAAGCTTTC.
Proper citation: RRID:Addgene_15682 Copy
Species: Homo sapiens
Genetic Insert: TIF1 gamma shRNA
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRetrosuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751102
Comments: shRNA oligos were cloned into pRetrosuper-Puro digested with BglII and HindIII.
Sense oligo: 5'-gatcccc GAAGATGTCT CAGAGTCTG ttcaagaga CAGACTCTGA GACATCTTC tttttggaaa-3'.
Proper citation: RRID:Addgene_15728 Copy
Species: Rattus norvegicus
Genetic Insert: rApoI
Vector Backbone Description: Vector Backbone:None; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:29991261
Comments: Esherichia coli strain that expresses MutaT7, a fusion of rat APOBEC1 and the T7 RNA polymerase. Genotype: DH10B Δung ΔmotAB ΔcsgABCDEFG [PlacI<>Ptac] Δ(araA-leu)7697::[PA1lacO-Tenth MutaT7]
Proper citation: RRID:Addgene_156460 Copy
Species: Homo sapiens
Genetic Insert: Myc shRNA
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRetrosuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17558397
Comments: hairpin targets the sequence: GATGAGGAAGAAATCGATG
Proper citation: RRID:Addgene_15662 Copy
Species: Synthetic
Genetic Insert: Nsp15
Vector Backbone Description: Backbone Size:6017; Vector Backbone:pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37704626
Comments: insert amino acid sequence: GLQSLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVETFYPKLx
Proper citation: RRID:Addgene_156481 Copy
Species: Homo sapiens
Genetic Insert: TIF1 gamma
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751102
Proper citation: RRID:Addgene_15734 Copy
Vector Backbone Description: Backbone Size:4816; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.111, Ligation number L3610.
Proper citation: RRID:Addgene_1575 Copy
Species: Homo sapiens
Genetic Insert: Fibroblast growth factor 2
Vector Backbone Description: Backbone Size:6377; Vector Backbone:pCAG-HA-puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32383752
Proper citation: RRID:Addgene_157663 Copy
Species: Mus musculus
Genetic Insert: mH2A.Z-EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pIND; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_15770 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp5
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157685 Copy
Species: Homo sapiens
Genetic Insert: hemicholinium-3-sensitive choline transporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11027560
Proper citation: RRID:Addgene_15766 Copy
Species: Homo sapiens
Genetic Insert: Jnk3a2
Vector Backbone Description: Backbone Size:5057; Vector Backbone:pGSTag; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8654373
Proper citation: RRID:Addgene_15748 Copy
Species: Homo sapiens
Genetic Insert: Jnk2a2
Vector Backbone Description: Backbone Size:5057; Vector Backbone:pGSTag; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8654373
Proper citation: RRID:Addgene_15744 Copy
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