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Species: Other
Genetic Insert: APEX2-NLS
Vector Backbone Description: Vector Backbone:pEF1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140281 Copy
Species: Parastagonospora nodorum elcA spacers, Lachnospiraceae bacterium Cas12a crRNA scaffold
Genetic Insert: crRNA array elcA
Vector Backbone Description: Backbone Marker:DOI: 10.1039/C8SC02870B; Backbone Size:14581; Vector Backbone:New backbone modified from pYFAC-pyroA; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32526136
Comments: To be contransformed with pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI in strains expressing dLbCas12a-VPR or dLbCas12a(D156R). These proof-of-concept vectors are intended to work as a positive control and aid the implementation of the CRISPRa vector set, facilitating initial testing and troubleshooting. The proof of concept activation is easily observable in Aspergillus nidulans with fluorescence microscopy.
Proper citation: RRID:Addgene_140203 Copy
Vector Backbone Description: Backbone Marker:MIT; Vector Backbone:pLentiCrispr-V2-mOrange (V2mO); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32053639
Comments: Lenti Crispr/Cas9 V2 with mOrange fluorescent marker, to make lentiviral production visible and target cell transduction visible with mOrange.
The cloning method for inserting a guide RNA (gRNA) is: BsmB1.
Proper citation: RRID:Addgene_140206 Copy
Species: Homo sapiens
Genetic Insert: Src deletion mutant
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684
Proper citation: RRID:Addgene_140298 Copy
Species: Homo sapiens
Genetic Insert: Src wildtype
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684
Proper citation: RRID:Addgene_140295 Copy
Species: Homo sapiens
Genetic Insert: Src deletion mutant
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684
Proper citation: RRID:Addgene_140296 Copy
Species: Mus musculus
Genetic Insert: XAF1 sgRNA
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_140290 Copy
Species: Homo sapiens
Genetic Insert: SSAT1
Vector Backbone Description: Vector Backbone:pOP-SVI-MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15905201
Proper citation: RRID:Addgene_140291 Copy
Species: Homo sapiens
Genetic Insert: DNA damage response
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31991288
Proper citation: RRID:Addgene_140219 Copy
Genetic Insert: zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
Vector Backbone Description: Vector Backbone:PB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32027839
Proper citation: RRID:Addgene_140222 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSLAX13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9608511
Comments: pSlax13 is a convenient entry vector for cloning into the pRCAS BP vectors. Clone your gene into pSlax13, then cut with ClaI to move your gene into pRCAS BP. pSlax13 has the advantage of allowing all the cloning steps to be carried out in a relatively small, high copy number vector.
pSlax13 was constructed by placing a ClaI adapter fragment into a Bluescript derivative. See author's map for information on the multiple cloning site.
For more information on the RCAS system, visit http://home.ncifcrf.gov/hivdrp/RCAS/index.html
Proper citation: RRID:Addgene_14029 Copy
Species: Synthetic
Genetic Insert: C-intein with miniVPR
Vector Backbone Description: Vector Backbone:PB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32027839
Proper citation: RRID:Addgene_140224 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSLAX13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9608511
Comments: pSlax13 is a convenient entry vector for cloning into the pRCAS BP vectors. Clone your gene into pSlax13, then cut with ClaI to move your gene into pRCAS BP. pSlax13 has the advantage of allowing all the cloning steps to be carried out in a relatively small, high copy number vector.
pSlax13 was constructed by placing a ClaI adapter fragment into a Bluescript derivative. See author's map for information on the multiple cloning site.
For more information on the RCAS system, visit http://home.ncifcrf.gov/hivdrp/RCAS/index.html
Proper citation: RRID:Addgene_14028 Copy
Species: Mus musculus
Genetic Insert: Lmx1b
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: EcoRI fragment containing full length mouse Lmx-1 cDNA, from Randy Johnson.
Proper citation: RRID:Addgene_14025 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSLAX13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9608511
Comments: pSlax13 is a convenient entry vector for cloning into the pRCAS BP vectors. Clone your gene into pSlax13, then cut with ClaI to move your gene into pRCAS BP. pSlax13 has the advantage of allowing all the cloning steps to be carried out in a relatively small, high copy number vector.
pSlax13 was constructed by placing a ClaI adapter fragment into a Bluescript derivative. See author's map for information on the multiple cloning site.
For more information on the RCAS system, visit http://home.ncifcrf.gov/hivdrp/RCAS/index.html
Proper citation: RRID:Addgene_14026 Copy
Species: Gallus gallus
Genetic Insert: Lmx1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For 3'UTR probe cut with Xba1 and use T7 RNA pol, from Bob Riddle.
Proper citation: RRID:Addgene_14024 Copy
Species: Mus musculus
Genetic Insert: Lef1
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Comments: From Gabrielle Kardon.
Proper citation: RRID:Addgene_14021 Copy
Species: Mus musculus
Genetic Insert: Tcf4B
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14667415
Comments: RCAS dnTcf4en virus was constructed using the mouse Tcf4B splice variant (gift of G. Dressler), as the chick Tcf4B splice variant is expressed in the developing limbs. The dominant-negative (dn) form was created by deleting amino acids 2–50, resulting in the production of a putative dominant-negative protein unable to bind and be activated by beta-catenin, but able to bind DNA via its C-terminal HMG box. The repressor domain from the Drosophila engrailed gene was fused in-frame to the 3′end of the dnTcf4 construct in the shuttle vector pSlax13 and then subcloned into retroviral vector RCAS(BP) A.
Proper citation: RRID:Addgene_14020 Copy
Genetic Insert: AntiCRISPR protein AcrVA1
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32027839
Proper citation: RRID:Addgene_140229 Copy
Species: Synthetic
Genetic Insert: Luciferase + crIFNg
Vector Backbone Description: Vector Backbone:PB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32027839
Proper citation: RRID:Addgene_140226 Copy
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