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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pEF-APEX2-NLS Resource Report Resource Website |
RRID:Addgene_140281 | APEX2-NLS | Other | Ampicillin | PMID:32353257 | Vector Backbone:pEF1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:07 | 0 | ||
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pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC Resource Report Resource Website |
RRID:Addgene_140203 | crRNA array elcA | Parastagonospora nodorum elcA spacers, Lachnospiraceae bacterium Cas12a crRNA scaffold | Ampicillin | PMID:32526136 | To be contransformed with pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI in strains expressing dLbCas12a-VPR or dLbCas12a(D156R). These proof-of-concept vectors are intended to work as a positive control and aid the implementation of the CRISPRa vector set, facilitating initial testing and troubleshooting. The proof of concept activation is easily observable in Aspergillus nidulans with fluorescence microscopy. | Backbone Marker:DOI: 10.1039/C8SC02870B; Backbone Size:14581; Vector Backbone:New backbone modified from pYFAC-pyroA; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:06 | 0 | |
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pLentiCrispr-V2-mOrange (V2mO) Resource Report Resource Website 1+ mentions |
RRID:Addgene_140206 | Ampicillin | PMID:32053639 | Lenti Crispr/Cas9 V2 with mOrange fluorescent marker, to make lentiviral production visible and target cell transduction visible with mOrange. The cloning method for inserting a guide RNA (gRNA) is: BsmB1. | Backbone Marker:MIT; Vector Backbone:pLentiCrispr-V2-mOrange (V2mO); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:06 | 3 | |||
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pDEST40-2XHA-Src-S4-S32-KD Resource Report Resource Website |
RRID:Addgene_140298 | Src deletion mutant | Homo sapiens | Ampicillin | PMID:30304684 | Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | lacks the UD region | 2026-09-01 09:20:07 | 0 | |
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pDEST40-2XFL-wtSrc Resource Report Resource Website |
RRID:Addgene_140295 | Src wildtype | Homo sapiens | Ampicillin | PMID:30304684 | Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:07 | 0 | ||
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pDEST40-2XHA-Src-S4-KD Resource Report Resource Website 1+ mentions |
RRID:Addgene_140296 | Src deletion mutant | Homo sapiens | Ampicillin | PMID:30304684 | Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | contains only the SH4 and KD regions | 2026-09-01 09:20:07 | 1 | |
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pKLV-XAF1 Resource Report Resource Website |
RRID:Addgene_140290 | XAF1 sgRNA | Mus musculus | Ampicillin | Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:07 | 0 | |||
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pOP/SSAT Resource Report Resource Website |
RRID:Addgene_140291 | SSAT1 | Homo sapiens | Ampicillin | PMID:15905201 | Vector Backbone:pOP-SVI-MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:07 | 0 | ||
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DDR_MKOv4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_140219 | DNA damage response | Homo sapiens | Ampicillin | PMID:31991288 | Backbone Marker:Addgene; Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:06 | 2 | ||
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pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA Resource Report Resource Website |
RRID:Addgene_140222 | zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR | Ampicillin | PMID:32027839 | Vector Backbone:PB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | D832A | 2026-09-01 09:20:06 | 0 | ||
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pSLAX13 HA His (CT#244) Resource Report Resource Website |
RRID:Addgene_14029 | Ampicillin | PMID:9608511 | pSlax13 is a convenient entry vector for cloning into the pRCAS BP vectors. Clone your gene into pSlax13, then cut with ClaI to move your gene into pRCAS BP. pSlax13 has the advantage of allowing all the cloning steps to be carried out in a relatively small, high copy number vector. pSlax13 was constructed by placing a ClaI adapter fragment into a Bluescript derivative. See author's map for information on the multiple cloning site. For more information on the RCAS system, visit http://home.ncifcrf.gov/hivdrp/RCAS/index.html | Backbone Size:3000; Vector Backbone:pSLAX13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:07 | 0 | |||
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pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA Resource Report Resource Website |
RRID:Addgene_140224 | C-intein with miniVPR | Synthetic | Ampicillin | PMID:32027839 | Vector Backbone:PB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:06 | 0 | ||
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pSLAX13 Flag His (CT#245) Resource Report Resource Website |
RRID:Addgene_14028 | Ampicillin | PMID:9608511 | pSlax13 is a convenient entry vector for cloning into the pRCAS BP vectors. Clone your gene into pSlax13, then cut with ClaI to move your gene into pRCAS BP. pSlax13 has the advantage of allowing all the cloning steps to be carried out in a relatively small, high copy number vector. pSlax13 was constructed by placing a ClaI adapter fragment into a Bluescript derivative. See author's map for information on the multiple cloning site. For more information on the RCAS system, visit http://home.ncifcrf.gov/hivdrp/RCAS/index.html | Backbone Size:3000; Vector Backbone:pSLAX13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:07 | 0 | |||
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pBS mLmx1 (CT#412) Resource Report Resource Website |
RRID:Addgene_14025 | Lmx1b | Mus musculus | Ampicillin | EcoRI fragment containing full length mouse Lmx-1 cDNA, from Randy Johnson. | Backbone Size:3000; Vector Backbone:pBS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:06 | 0 | ||
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pSLAX13 Flag (CT#231) Resource Report Resource Website |
RRID:Addgene_14026 | Ampicillin | PMID:9608511 | pSlax13 is a convenient entry vector for cloning into the pRCAS BP vectors. Clone your gene into pSlax13, then cut with ClaI to move your gene into pRCAS BP. pSlax13 has the advantage of allowing all the cloning steps to be carried out in a relatively small, high copy number vector. pSlax13 was constructed by placing a ClaI adapter fragment into a Bluescript derivative. See author's map for information on the multiple cloning site. For more information on the RCAS system, visit http://home.ncifcrf.gov/hivdrp/RCAS/index.html | Backbone Size:3000; Vector Backbone:pSLAX13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:07 | 0 | |||
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pBS cLmx1 (CT#338) Resource Report Resource Website |
RRID:Addgene_14024 | Lmx1 | Gallus gallus | Ampicillin | For 3'UTR probe cut with Xba1 and use T7 RNA pol, from Bob Riddle. | Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:06 | 0 | ||
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RCAS(A) mLef1 (CT#1141) Resource Report Resource Website |
RRID:Addgene_14021 | Lef1 | Mus musculus | Ampicillin | From Gabrielle Kardon. | Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:06 | 0 | ||
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RCAS(A) mdnTcf4Ben (CT#1130) Resource Report Resource Website |
RRID:Addgene_14020 | Tcf4B | Mus musculus | Ampicillin | PMID:14667415 | RCAS dnTcf4en virus was constructed using the mouse Tcf4B splice variant (gift of G. Dressler), as the chick Tcf4B splice variant is expressed in the developing limbs. The dominant-negative (dn) form was created by deleting amino acids 2–50, resulting in the production of a putative dominant-negative protein unable to bind and be activated by beta-catenin, but able to bind DNA via its C-terminal HMG box. The repressor domain from the Drosophila engrailed gene was fused in-frame to the 3′end of the dnTcf4 construct in the shuttle vector pSlax13 and then subcloned into retroviral vector RCAS(BP) A. | Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin | Dominant negative Tcf4B, engrailed fusion | 2026-09-01 09:20:06 | 0 |
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pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE Resource Report Resource Website |
RRID:Addgene_140229 | AntiCRISPR protein AcrVA1 | Ampicillin | PMID:32027839 | Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:06 | 0 | |||
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pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE) Resource Report Resource Website |
RRID:Addgene_140226 | Luciferase + crIFNg | Synthetic | Ampicillin | PMID:32027839 | Vector Backbone:PB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:20:06 | 0 |
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