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Species: Synthetic
Genetic Insert: N-BLa 1.1 fusion protein
Vector Backbone Description: Backbone Size:4083; Vector Backbone:pACYC184 (modified); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28266525
Comments: TMD: Integrin alphaV mut GP, contains ApaI restriction site
Proper citation: RRID:Addgene_88994 Copy
Species: Synthetic
Genetic Insert: toxin-antitoxin + LuxABCDE
Vector Backbone Description: Backbone Marker: Nobuhiko Okada (PMID: 9846743); Vector Backbone:pLZ12Km2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24061415
Comments: This plasmid contains a toxin-antitoxin system that stabilizes the plasmid in Streptococcus by post-segregational killing of bacteria that have lost the plasmid. This stabilization improves plasmid utility in animal infection models. For more information, please see the original reference. Although designed for use with Streptococcus pyogenes, this plasmid also labels other streptococcal species. For more information, please see Loh JM and Proft T (2016). PMID: 26410784
Proper citation: RRID:Addgene_88902 Copy
Species: Synthetic
Genetic Insert: toxin-antitoxin + Red-shifted firefly luciferase
Vector Backbone Description: Backbone Marker: Nobuhiko Okada (PMID: 9846743); Vector Backbone:pLZ12Km2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24061415
Comments: This plasmid contains a toxin-antitoxin system that stabilizes the plasmid in Streptococcus by post-segregational killing of bacteria that have lost the plasmid. This stabilization improves plasmid utility in animal infection models. For more information, please see the original reference. Although designed for use with Streptococcus pyogenes, this plasmid also labels other streptococcal species. For more information, please see Loh JM and Proft T (2016). PMID: 26410784
Proper citation: RRID:Addgene_88901 Copy
Species: Synthetic
Genetic Insert: sf-GFP-GGS9-Cre-6xHis
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5073; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_89343 Copy
Species: Synthetic
Genetic Insert: His6-(pos16)-GFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5152; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22840771
Proper citation: RRID:Addgene_89249 Copy
Species: Synthetic
Genetic Insert: His6-(pos15)-GFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5152; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22840771
Proper citation: RRID:Addgene_89248 Copy
Species: Synthetic
Genetic Insert: Cpf1, RR variant
Vector Backbone Description: Backbone Size:4328; Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28581492
Comments: This variant also recognizes CCCC and ACCC PAMs (and, with lower efficiency, VYCV).
Contains a U6-crRNA expression cassette (BbsI restriction sites for guide cloning).
For more information on Zhang lab CRISPR plasmids, please refer to www.addgene.org/crispr/zhang/.
Proper citation: RRID:Addgene_89352 Copy
Species: Synthetic
Genetic Insert: Cpf1, RR variant
Vector Backbone Description: Backbone Size:5402; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28581492
Comments: For more information on Zhang lab CRISPR plasmids, please refer to www.addgene.org/crispr/zhang/.
Proper citation: RRID:Addgene_89355 Copy
Species: Synthetic
Genetic Insert: Cpf1 RVR variant
Vector Backbone Description: Backbone Size:4328; Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28581492
Comments: This variant also recognizes TTTV and RATR PAMs (R = A or G).
Contains a U6-crRNA expression cassette (BbsI restriction sites for guide cloning).
For more information on Zhang lab CRISPR plasmids, please refer to www.addgene.org/crispr/zhang/.
Proper citation: RRID:Addgene_89354 Copy
Species: Synthetic
Genetic Insert: His6-(pos16)-GFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5152; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22840771
Proper citation: RRID:Addgene_89252 Copy
Species: Synthetic
Genetic Insert: His6-(pos16)-GFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5152; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_89250 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pRFHUE; Vector Types:Binary plasmid for ATMT of filamentous fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21663991
Comments: Binary plasmid for ATMT of filamentous fungi. The T-DNA contains a Hygromycin resistance gene under the control of A. nidulans trpC gen promoter and terminator
Proper citation: RRID:Addgene_89469 Copy
Species: Synthetic
Genetic Insert: Asparaginyl endopeptidase from Oldenlandia affinis
Vector Backbone Description: Backbone Size:4540; Vector Backbone:pHYRSF53; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28369872
Proper citation: RRID:Addgene_89482 Copy
Species: Synthetic
Genetic Insert: CeNL
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27966527
Comments: Derived from NanoLuc luciferase
Proper citation: RRID:Addgene_89509 Copy
Species: Synthetic
Genetic Insert: OeNL
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27966527
Comments: Derived from NanoLuc luciferase
Proper citation: RRID:Addgene_89528 Copy
Species: Synthetic
Genetic Insert: OeNL
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27966527
Comments: Derived from NanoLuc luciferase
Proper citation: RRID:Addgene_89527 Copy
Species: Synthetic
Genetic Insert: OeNL
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27966527
Comments: Derived from NanoLuc luciferase
Proper citation: RRID:Addgene_89526 Copy
Species: Synthetic
Genetic Insert: YeNL
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27966527
Comments: Derived from NanoLuc luciferase
Proper citation: RRID:Addgene_89521 Copy
Species: Synthetic
Genetic Insert: OeNL
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27966527
Comments: Derived from NanoLuc luciferase
Proper citation: RRID:Addgene_89524 Copy
Species: Synthetic
Genetic Insert: OeNL
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27966527
Comments: Derived from NanoLuc luciferase
Proper citation: RRID:Addgene_89523 Copy
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