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Species: Homo sapiens
Genetic Insert: H6hSDHA
Vector Backbone Description: Vector Backbone:pET_Duet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27296776
Proper citation: RRID:Addgene_113045 Copy
Species: Homo sapiens
Genetic Insert: GPCR activation based DA sensor GRAB_DA1m
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:4495; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30007419
Proper citation: RRID:Addgene_113049 Copy
Species: Homo sapiens
Genetic Insert: hSDH5_C83D
Vector Backbone Description: Vector Backbone:pET41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27296776
Proper citation: RRID:Addgene_113048 Copy
Species: Homo sapiens
Genetic Insert: hSDH5_G78R
Vector Backbone Description: Vector Backbone:pET41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27296776
Proper citation: RRID:Addgene_113047 Copy
Species: Homo sapiens
Genetic Insert: p21 Activated kinase 4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29983868
Proper citation: RRID:Addgene_113088 Copy
Species: Homo sapiens
Genetic Insert: p21 Activated kinase 4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29983868
Proper citation: RRID:Addgene_113090 Copy
Species: Homo sapiens
Genetic Insert: RapGEF3
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11801596
Proper citation: RRID:Addgene_113110 Copy
Species: Homo sapiens
Genetic Insert: prostatic acid phosphatase isoform 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233530
Comments: The plasmid contains a V360A compared to the reference NP_001090.2 that is not of concern for function.
Proper citation: RRID:Addgene_113052 Copy
Species: Homo sapiens
Genetic Insert: human Kv2.1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113056 Copy
Species: Homo sapiens
Genetic Insert: EGFR (del3)
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16187797
Comments: Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. Mutation made by using the following primers to PCR from plasmid 11011: 5'-GTCGCTATCAAGGAACCAACATCTCCGAAA-3' and 5'-TTTCGGAGATGTTGGTTCCTTGATAGCGAC-3'
Proper citation: RRID:Addgene_11015 Copy
Species: Homo sapiens
Genetic Insert: hCCR5
Vector Backbone Description: Vector Backbone:mTFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30150645
Proper citation: RRID:Addgene_110193 Copy
Species: Homo sapiens
Genetic Insert: hCXCR4
Vector Backbone Description: Vector Backbone:mTFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37591879
Proper citation: RRID:Addgene_110196 Copy
Species: Homo sapiens
Genetic Insert: EGFR D837A
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16187797
Comments: Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. Point mutation made by using the following primers to PCR from plasmid 11011: 5'-GGTGCACCGCGCCCTGGCAGCCA-3' and 5'-TGGCTGCCAGGGCGCGGTGCACC-3'
Proper citation: RRID:Addgene_11014 Copy
Species: Homo sapiens
Genetic Insert: hCCR5
Vector Backbone Description: Vector Backbone:LSSmOrange-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37591879
Proper citation: RRID:Addgene_110194 Copy
Species: Homo sapiens
Genetic Insert: fibroblast growth factor receptor 2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6300; Vector Backbone:p3xFlag-CMV-13; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_110110 Copy
Species: Homo sapiens
Genetic Insert: fibroblast growth factor receptor 2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6300; Vector Backbone:p3xFlag-CMV-13; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30761385
Proper citation: RRID:Addgene_110105 Copy
Species: Homo sapiens
Genetic Insert: fibroblast growth factor receptor 2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6300; Vector Backbone:p3xFlag-CMV-13; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_110106 Copy
Species: Homo sapiens
Genetic Insert: fibroblast growth factor receptor 2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6300; Vector Backbone:p3xFlag-CMV-13; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_110107 Copy
Species: Homo sapiens
Genetic Insert: fibroblast growth factor receptor 2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6300; Vector Backbone:p3xFlag-CMV-13; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_110108 Copy
Species: Homo sapiens
Genetic Insert: SPRTN
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25261934
Comments: According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T
Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant.
Proper citation: RRID:Addgene_110215 Copy
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