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Species: Synthetic
Genetic Insert: zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32027839
Proper citation: RRID:Addgene_140227 Copy
Genetic Insert: phytoene-beta carotene synthase
Vector Backbone Description: Vector Backbone:LCB7; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Comments: The minor differences between the sequence and .gb file are of no functional concern.
Proper citation: RRID:Addgene_140232 Copy
Species: Mus musculus
Genetic Insert: PI3K regulatory subunit p55alpha
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Cantley lab recommends growth at room temperature.
Proper citation: RRID:Addgene_1402 Copy
Species: Synthetic
Genetic Insert: non-targeting sgRNA
Vector Backbone Description: Vector Backbone:Lenti-eCas9; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31851912
Proper citation: RRID:Addgene_140238 Copy
Species: Synthetic
Genetic Insert: sgRNA targeting human MIB1
Vector Backbone Description: Vector Backbone:Lenti-eCas9; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31851912
Proper citation: RRID:Addgene_140239 Copy
Species: E. coli, S. pyogenes, P. marinus
Genetic Insert: SPACE-ΔUGI
Vector Backbone Description: Backbone Marker:pCMV-ABEmax-P2A-EGFP (Addgene #112101); Backbone Size:3400; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32483364
Proper citation: RRID:Addgene_140243 Copy
Species: Homo sapiens
Genetic Insert: Tet-inducible mEGFP-MIB1
Vector Backbone Description: Backbone Size:8011; Vector Backbone:pLVX-BSD-tet; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31851912
Proper citation: RRID:Addgene_140241 Copy
Species: E. coli, S. pyogenes, P. marinus
Genetic Insert: SPACE
Vector Backbone Description: Backbone Marker:pCMV-ABEmax-P2A-EGFP (Addgene #112101); Backbone Size:3400; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32483364
Proper citation: RRID:Addgene_140242 Copy
Species: Rattus norvegicus
Genetic Insert: eUNG-BE4max(R33A)_VRQR-ΔUGI
Vector Backbone Description: Backbone Marker:pCMV-ABEmax-P2A-EGFP (Addgene #112101); Backbone Size:3400; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32690971
Proper citation: RRID:Addgene_140254 Copy
Species: Rattus norvegicus
Genetic Insert: BE4max(R33A)_VRQR-ΔUGI
Vector Backbone Description: Backbone Marker:pCMV-ABEmax-P2A-EGFP (Addgene #112101); Backbone Size:3400; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32690971
Proper citation: RRID:Addgene_140255 Copy
Species: Rattus norvegicus
Genetic Insert: eUNG-BE4max(R33A)_NG-ΔUGI
Vector Backbone Description: Backbone Marker:pCMV-ABEmax-P2A-EGFP (Addgene #112101); Backbone Size:3400; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32690971
Proper citation: RRID:Addgene_140256 Copy
Species: Rattus norvegicus
Genetic Insert: eUNG-BE4max(R33A)-ΔUGI
Vector Backbone Description: Backbone Marker:pCMV-ABEmax-P2A-EGFP (Addgene #112101); Backbone Size:3400; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32690971
Proper citation: RRID:Addgene_140252 Copy
Species: Synthetic
Genetic Insert: Two MS2CP-binding sites embedded in scaffold
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: scMS2(WTx2) motif was inserted between BamHI and AgeI sites of pAptamerCassette-EGFP (ID: 140288).
Proper citation: RRID:Addgene_140258 Copy
Species: Other
Genetic Insert: T7-lacO-QacR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140389 Copy
Species: Synthetic
Genetic Insert: T7-otrO-3WJdB-T
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140382 Copy
Species: Synthetic
Genetic Insert: T7-ctcO-3WJdB-T
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140384 Copy
Species: Other
Genetic Insert: T7-lacO-MphR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140385 Copy
Species: Synthetic
Genetic Insert: T7-14BP-tetO-3WJdB-T
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140380 Copy
Species: Other
Genetic Insert: T7-lacO-OtrR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140381 Copy
Species: Homo sapiens
Genetic Insert: eGFP-2A-Tau
Vector Backbone Description: Backbone Size:6468; Vector Backbone:AAV.CBA.WPRE; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31249873
Comments: Please Note: Plasmid contains a 152bp deletion in the backbone that removes the first 84bp of the ITR upstream of the CAG promoter. This deletion is not known to affect plasmid function. Plasmid 240284 (https://www.addgene.org/240284/) is an alternative version with a complete ITR.
Proper citation: RRID:Addgene_140424 Copy
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