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Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:Backbone derived from pEF-GFP (Addgene #11154); Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33317513
Proper citation: RRID:Addgene_164411 Copy
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC19; Vector Types:Bacterial vector for DNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33590100
Comments: Please cite the following article in any publications where this construct is utilized: doi: 10.1093/nar/gkab070
Z. Adhireksan, D. Sharma, P.L. Lee, Q. Bao, S. Padavattan, W.K. Shum, G.E. Davey & C.A. Davey. (2021). Engineering Nucleosomes for Generating Diverse Chromatin Assemblies. Nucleic Acids Res.
Proper citation: RRID:Addgene_164493 Copy
Genetic Insert: sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33317513
Proper citation: RRID:Addgene_164414 Copy
Genetic Insert: ABEmax
Vector Backbone Description: Vector Backbone:Derived from pCMV-ABEmax (Addgene #112095); Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33317513
Proper citation: RRID:Addgene_164415 Copy
Species: Gallus gallus
Genetic Insert: TVA
Vector Backbone Description: Backbone Size:4500; Vector Backbone:biCS2 (bicistronic pCS2); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34099488
Proper citation: RRID:Addgene_164486 Copy
Species: Danio rerio
Genetic Insert: ythdf1
Vector Backbone Description: Vector Backbone:pSP64T; Vector Types:Production of mRNA for zebrafish injection.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378672
Proper citation: RRID:Addgene_164488 Copy
Species: Homo sapiens
Genetic Insert: WAF1 stop codon mut
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8242752
Comments: PCR-generated cDNA insert cloned into the NotI site of pCEP4.
Proper citation: RRID:Addgene_16449 Copy
Species: Homo sapiens
Genetic Insert: WAF1 antisense
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8242752
Comments: PCR-generated cDNA insert cloned into the NotI site of pCEP4.
pCEP-WAF1-AS is an antisense expression plasmid. Full-length WAF1 was cloned in opposite orientation in expression plasmid.
Proper citation: RRID:Addgene_16448 Copy
Genetic Insert: SARS-CoV-2 Spike
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33412089
Proper citation: RRID:Addgene_164435 Copy
Species: Firefly
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33412089
Proper citation: RRID:Addgene_164432 Copy
Species: Caenorhabditis elegans
Genetic Insert: C.elegans Tandem pore domain K+ channel twk-16
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pOX; Vector Types:Xenopus Oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11301195
Proper citation: RRID:Addgene_16444 Copy
Genetic Insert: p53-binding site (mutant)
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8242752
Comments: MG15-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream.
MG15 contains 15 copies of a mutated p53-binding sequence. Addgene has confirmed the presence of 8 of these copies.
Direct repeats of the oligonucleotide MG (5'-CCTTAATGGACTTTAATGG-
3') were used for the
p53 nonbinding control sequences.
Proper citation: RRID:Addgene_16443 Copy
Species: Homo sapiens
Genetic Insert: MDM2
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1614537
Comments: Full-length human MDM2 cDNA.
Proper citation: RRID:Addgene_16441 Copy
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2144057
Comments: The vector pCMV-Neo-Bam was engineered to contain two independent transcription units. The first unit is comprised of a CMV promoter/enhancer upstream of a multiple cloning site, and splice and polyA sites to ensure appropriate processing. The second transcription unit includes a herpes simplex virus (HSV) thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.
Proper citation: RRID:Addgene_16440 Copy
Vector Backbone Description: Backbone Size:11536; Vector Backbone:lentiCRISPRV2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33264619
Proper citation: RRID:Addgene_164273 Copy
Genetic Insert: SARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33412089
Proper citation: RRID:Addgene_164437 Copy
Species: Homo sapiens
Genetic Insert: WAF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8242752
Comments: full-length WAF1 cDNA insert cloned into the NotI site of pCEP4.
Proper citation: RRID:Addgene_16450 Copy
Vector Backbone Description: Backbone Size:3726; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD123.102, Ligation number L4138.
Proper citation: RRID:Addgene_1645 Copy
Species: Mus musculus
Genetic Insert: Tgfb1
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Backbone Size:6062; Vector Backbone:AAV-MCS8; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32352930
Proper citation: RRID:Addgene_164427 Copy
Species: Mus musculus
Genetic Insert: CD200
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Backbone Size:6062; Vector Backbone:AAV-MCS8; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31036641
Proper citation: RRID:Addgene_164425 Copy
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