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Species: Synthetic
Genetic Insert: Capri(1-10)
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33637968
Proper citation: RRID:Addgene_165142 Copy
Species: Homo sapiens
Genetic Insert: UbeE1L
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.
Proper citation: RRID:Addgene_165099 Copy
Species: Influenza A virus
Genetic Insert: Influenza B virus(B/Georgia/05/2019) nucleocapsid protein
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165093 Copy
Species: SARS-CoV2
Genetic Insert: Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166
Vector Backbone Description: Vector Backbone:pET-32a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165094 Copy
Species: SARS-CoV2
Genetic Insert: Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173)
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165091 Copy
Species: Influenza A virus
Genetic Insert: Influenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165092 Copy
Species: SARS-CoV2
Genetic Insert: Severe acute respiratory syndrome coronavirus 2 spike protein
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165096 Copy
Species: Rattus norvegicus
Genetic Insert: mEGFP(A206K)-paCaMKII(K42M)
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Comments: Only tested in HeLa cells, but not in neurons.
Proper citation: RRID:Addgene_165440 Copy
Species: Other
Genetic Insert: EGFP-NLS
Vector Backbone Description: Backbone Marker:Roger Tsien; Vector Backbone:AAV-flex-ReaChR-citrine (Addgene 50955); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34176926
Proper citation: RRID:Addgene_165441 Copy
Species: Rattus norvegicus
Genetic Insert: tdTomato-P2A-paCaMKII
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Proper citation: RRID:Addgene_165431 Copy
Species: Rattus norvegicus
Genetic Insert: tdTomato-P2A-paCaMKII(T286A)
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Proper citation: RRID:Addgene_165432 Copy
Species: Rattus norvegicus
Genetic Insert: FHS-paCaMKII(SD)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Proper citation: RRID:Addgene_165430 Copy
Species: Homo sapiens
Genetic Insert: ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Proper citation: RRID:Addgene_165435 Copy
Species: Rattus norvegicus
Genetic Insert: tdTomato-P2A-paCaMKII(K42M)
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Proper citation: RRID:Addgene_165433 Copy
Species: Homo sapiens
Genetic Insert: ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Proper citation: RRID:Addgene_165434 Copy
Species: Rattus norvegicus
Genetic Insert: mEGFP(A206K)-paCaMKII
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Comments: Only tested in HeLa cells, but not in neurons.
Proper citation: RRID:Addgene_165438 Copy
Species: Other
Genetic Insert: 9 degrees N-7 DNA Polymerase chain terminating
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: 9 Degrees North DNA polymerase variant with an enhanced ability to incorporate modified substrates such as dideoxynucleotides, ribonucleotides and acyclonucleotides. Comparable to Therminator DNA Pol at NEB.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165502 Copy
Species: Other
Genetic Insert: DNA Ligase IV
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Ligase used in efficiently joining single-strand breaks in a double-strand polydeoxynucleotide in an ATP-dependent reaction. Also involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165503 Copy
Species: Other
Genetic Insert: HindIII
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Type II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165508 Copy
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC19; Vector Types:Bacterial vector for DNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33590100
Comments: Please cite the following article in any publications where this construct is utilized:
doi: 10.1093/nar/gkab070
Z. Adhireksan, D. Sharma, P.L. Lee, Q. Bao, S. Padavattan, W.K. Shum, G.E. Davey & C.A. Davey. (2021). Engineering Nucleosomes for Generating Diverse Chromatin Assemblies. Nucleic Acids Res.
Proper citation: RRID:Addgene_165452 Copy
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