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Species: Streptoalloteichus hindustanus
Genetic Insert: ble (bleomycin resistance marker)
Vector Backbone Description: Vector Backbone:custom; Vector Types:Bacterial Expression, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Comments: AMA1 sequence contains multiple ambiguous bases that are likely mixed nucleotide populations. AMA1 is a palindrome and thus difficult to sequence. Dep. confirms the mixed population does not affect plasmid function.
Proper citation: RRID:Addgene_127976 Copy
Genetic Insert: traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites
Vector Backbone Description: Vector Backbone:pBS; Vector Types:Drosophila OSC reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128010 Copy
Species: Drosophila melanogaster
Genetic Insert: Gal4-DBD-3xFlag
Vector Backbone Description: Vector Backbone:modified from pAcM; Vector Types:Gal4 entry vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128014 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia response element (x3) from PGK1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pGL4.22; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29861175
Proper citation: RRID:Addgene_128095 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments:
Proper citation: RRID:Addgene_128073 Copy
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_128060 Copy
Vector Backbone Description: Vector Backbone:plenti guide puro addgene 52963; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128063 Copy
Vector Backbone Description: Vector Backbone:pLenti6-V5-Topo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The V5 tag in this plasmid backbone is not fused to mCherry.
Proper citation: RRID:Addgene_128062 Copy
Vector Backbone Description: Vector Backbone:pLVX-Tet3G; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128061 Copy
Vector Backbone Description: Vector Backbone:pCDH-EF1-IRES cop GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Addgene NGS analysis identified 3 lentiviral ITR sequences. Depositing lab has indicated that this plasmid is functional.
Proper citation: RRID:Addgene_128059 Copy
Vector Backbone Description: Vector Backbone:pLEX Mcs; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128045 Copy
Vector Backbone Description: Vector Backbone:pcdna3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128047 Copy
Genetic Insert: sgRNA AAVS1 + GFP
Vector Backbone Description: Vector Backbone:AAV; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32076642
Proper citation: RRID:Addgene_128119 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::ScCas9 (wheat codon optimized):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128192 Copy
Species: Rhodosporidium toruloides
Genetic Insert: gRNA cloning cassette
Vector Backbone Description: Backbone Size:6303; Vector Backbone:pZPK; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31038202
Proper citation: RRID:Addgene_128178 Copy
Species: Rhodosporidium toruloides
Genetic Insert: SpCas9-NLS3
Vector Backbone Description: Backbone Marker:Zhao lab; Backbone Size:13658; Vector Backbone:pCAMBRIA; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31038202
Proper citation: RRID:Addgene_128177 Copy
Species: Homo sapiens
Genetic Insert: shcGAS Mmu
Vector Backbone Description: Backbone Size:13320; Vector Backbone:pTRIPZ RFP+; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28598415
Proper citation: RRID:Addgene_128175 Copy
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pAWP78; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26902345
Comments: pAWP78 was gifted from Mary Lidstrom's laboratory at the University of Washington
Proper citation: RRID:Addgene_128162 Copy
Species: Other
Genetic Insert: Sar1
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33852913
Comments: Sar1-T39N is a dominant-negative mutant
Proper citation: RRID:Addgene_128155 Copy
Species: Rattus norvegicus
Genetic Insert: Dynamin 2
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19948954
Comments: Please note: Plasmid contains D406G, V664A, H692R, and Q758R mutations in Dynamin2 compared to the NCBI reference sequence XP_006242670.1. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_128153 Copy
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