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Genetic Insert: GFP-Avi tag
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27506200
Proper citation: RRID:Addgene_73088 Copy
Species: Homo sapiens
Genetic Insert: E2F7
Vector Backbone Description: Vector Backbone:pcDNA3.1V5hisTOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26957605
Proper citation: RRID:Addgene_73074 Copy
Species: Rattus norvegicus
Genetic Insert: BE3
Vector Backbone Description: Backbone Marker:origene; Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27096365
Proper citation: RRID:Addgene_73021 Copy
Species: Homo sapiens
Genetic Insert: VEGFA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7200; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_73017 Copy
Species: Rattus norvegicus
Genetic Insert: BE1
Vector Backbone Description: Backbone Marker:origene; Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27096365
Proper citation: RRID:Addgene_73019 Copy
Species: Bacillus cereus ATCC 14579
Genetic Insert: pET24a
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21531795
Comments: Cells were harvested via centrifugation, resus-pended in 50 mM Tris-HCl, pH 7.5, 1 mM EDTA, and 0.1 mMdithiothreitol(DTT) (TED), and frozen at -80°C. Following thawing, cells were lysed bymicrofluidization and cell debris was removed by centrifugation. The lysate wasfractionated by first bringing the solution to 30% saturation, discarding thepellet, and then subsequently increasing the saturation to 60% and 90% with(NH4)2SO4and dissolving the precipitates in TED. The protein was further
purified using hydrophobic interaction chromatography over a HiPrep 16/10phenyl FF (high sub) column (GE Healthcare), dialyzed against TED, andsubjected to anion exchange chromatography over a Source Q column (GEHealthcare). The eluted protein was passed over a HiPrep 26/60 Sephacryl S100HR column (GE Healthcare) for size exclusion.
The structure was determined by X-ray Crystallography and deposited in the PDB as 3Q1P 3Q4I
Proper citation: RRID:Addgene_73161 Copy
Species: Homo sapiens
Genetic Insert: COXIV-COX8-dL5-2XG4S-mCer3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5388; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650487
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
Details of mCerulean3 can be found in:
Markwardt, M. L., Kremers, G. J., Kraft, C. A., Ray, K., Cranfill, P. J., Wilson, K. A., Day, R. N., Wachter, R. M., Davidson, M. W., and Rizzo, M. A. (2011) An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching. PLoS One 6, e17896.
For more information about this plasmid, please see the associated publication (PMID: 25650487) as well as He et al. Nat Methods. 2016 Jan 25. doi: 10.1038/nmeth.3735. [Epub ahead of print] PMID: 26808669.
Proper citation: RRID:Addgene_73208 Copy
Species: Homo sapiens
Genetic Insert: kappa-myc-dL5-2XG4S-mCer3-KDEL
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5393; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650487
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
Details of mCerulean3 can be found in:
Markwardt, M. L., Kremers, G. J., Kraft, C. A., Ray, K., Cranfill,
P. J., Wilson, K. A., Day, R. N., Wachter, R. M., Davidson, M. W., and
Rizzo, M. A. (2011) An Improved Cerulean Fluorescent Protein with
Enhanced Brightness and Reduced Reversible Photoswitching. PLoS
One 6, e17896.
Proper citation: RRID:Addgene_73209 Copy
Species: Homo sapiens
Genetic Insert: kappa-myc-dL5-2XG4S-TM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5388; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650487
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
For more information about this plasmid, please see the associated publication (PMID: 25650487) as well as He et al. Nat Methods. 2016 Jan 25. doi: 10.1038/nmeth.3735. [Epub ahead of print] PMID: 26808669.
Proper citation: RRID:Addgene_73206 Copy
Species: Homo sapiens
Genetic Insert: KozATG-dL5-2XG4S-mCer3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5382; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650487
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
Details of mCerulean3 can be found in:
Markwardt, M. L., Kremers, G. J., Kraft, C. A., Ray, K., Cranfill, P. J., Wilson, K. A., Day, R. N., Wachter, R. M., Davidson, M. W., and Rizzo, M. A. (2011) An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching. PLoS One 6, e17896.
For more information about this plasmid, please see the associated publication (PMID: 25650487) as well as He et al. Nat Methods. 2016 Jan 25. doi: 10.1038/nmeth.3735. [Epub ahead of print] PMID: 26808669.
Proper citation: RRID:Addgene_73207 Copy
Species: Homo sapiens
Genetic Insert: FKBP(F36V)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21768107
Proper citation: RRID:Addgene_73180 Copy
Species: S. pyogenes
Genetic Insert: dCas9 from S. pyogenes
Vector Backbone Description: Backbone Marker:Takara; Vector Backbone:pMD19T simple; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26689101
Proper citation: RRID:Addgene_73223 Copy
Species: S. pyogenes
Genetic Insert: dCas9 from S. pyogenes
Vector Backbone Description: Backbone Marker:Takara; Vector Backbone:pMD19T simple; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26689101
Proper citation: RRID:Addgene_73220 Copy
Species: S. pyogenes MGAS5005
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pMCSCen1; Vector Types:Bacterial Expression, CRISPR, in Y. lipolytica; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27349768
Proper citation: RRID:Addgene_73226 Copy
Species: Homo sapiens
Genetic Insert: SNORD116-13
Vector Backbone Description: Backbone Marker:Zefeng Wang's lab; Backbone Size:4700; Vector Backbone:pZW1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25378317
Comments: Two snoRNA genes SNORD116-13 and SNORD116-14 from the Prader–Willi syndrome deletion region flanked by multiple cloning sites were inserted into the weak intron of Enhanced Green Fluoresence Protein (EGFP) such that only proper splicing leads to EGFP fluorescence.
Proper citation: RRID:Addgene_73174 Copy
Genetic Insert: 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls
Vector Backbone Description: Backbone Marker:Feng Zhang lab (Addgene plasmid #52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: For protocols and more information about this library please see https://www.addgene.org/pooled-library/broadgpp-crispr-knockout/
Proper citation: RRID:Addgene_73178 Copy
Species: Mus musculus
Genetic Insert: BKalpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5401; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26301573
Proper citation: RRID:Addgene_73213 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5384; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25490520
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
Proper citation: RRID:Addgene_73214 Copy
Genetic Insert: PrhaB-mqsR
Vector Backbone Description: Backbone Marker:Barry L. Wanner; Backbone Size:3267; Vector Backbone:pKD4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25800749
Proper citation: RRID:Addgene_73194 Copy
Species: Mus musculus
Genetic Insert: spCas9
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25642960
Comments: We cloned 2 guide RNAs and a puromycin cassette.
Proper citation: RRID:Addgene_73131 Copy
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