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Species: Homo sapiens
Genetic Insert: Barcoded piggyBac Puro SRT
Vector Backbone Description: Vector Backbone:PB-SRT-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36062164
Proper citation: RRID:Addgene_193162 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac Puro SRT
Vector Backbone Description: Vector Backbone:PB-SRT-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36062164
Proper citation: RRID:Addgene_193163 Copy
Species: Homo sapiens
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36250010
Proper citation: RRID:Addgene_213722 Copy
Species: Homo sapiens
Genetic Insert: TAZ
Vector Backbone Description: Backbone Size:12153; Vector Backbone:p-Inducer10b; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37934103
Proper citation: RRID:Addgene_213587 Copy
Species: Homo sapiens
Genetic Insert: TAZ
Vector Backbone Description: Backbone Size:12153; Vector Backbone:p-Inducer10b; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37934103
Proper citation: RRID:Addgene_213588 Copy
Species: Homo sapiens
Genetic Insert: TAZ
Vector Backbone Description: Backbone Size:12153; Vector Backbone:p-Inducer10b; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37934103
Proper citation: RRID:Addgene_213589 Copy
Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Backbone Size:12153; Vector Backbone:p-Inducer10b; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37934103
Proper citation: RRID:Addgene_213583 Copy
Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Backbone Size:12153; Vector Backbone:p-Inducer10b; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37934103
Proper citation: RRID:Addgene_213585 Copy
Species: Homo sapiens
Genetic Insert: TAZ
Vector Backbone Description: Backbone Size:12153; Vector Backbone:p-Inducer10b; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37934103
Proper citation: RRID:Addgene_213590 Copy
Species: Homo sapiens
Genetic Insert: CBLB:PBC020-G02:C248501
Vector Backbone Description: Vector Backbone:pET28-MHL (Addgene #26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PBD link: https://www.rcsb.org/structure/8GCY
Proper citation: RRID:Addgene_214440 Copy
Species: Homo sapiens
Genetic Insert: CAG_iPE-N_bGH
Vector Backbone Description: Backbone Size:2828; Vector Backbone:pBlueScript; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38302659
Proper citation: RRID:Addgene_214734 Copy
Species: Homo sapiens
Genetic Insert: CAG_iPE-C_bGH
Vector Backbone Description: Backbone Size:2825; Vector Backbone:pBlueScript; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38302659
Proper citation: RRID:Addgene_214735 Copy
Species: Homo sapiens
Genetic Insert: ZFYVE27
Vector Backbone Description: Vector Backbone:pHRsinUbEm; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_214471 Copy
Species: Homo sapiens
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pLKO.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36250010
Proper citation: RRID:Addgene_213717 Copy
Species: Homo sapiens
Genetic Insert: PRF1-Exon3 HDRT
Vector Backbone Description: Backbone Size:3155; Vector Backbone:pDonor; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38306418
Proper citation: RRID:Addgene_209075 Copy
Species: Homo sapiens
Genetic Insert: miniTurbo-V5-CSNK1G3
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_214460 Copy
Species: Homo sapiens
Genetic Insert: miniTurbo-V5-PRPF4B
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_214463 Copy
Species: Homo sapiens
Genetic Insert: STK17A-V5-miniTurbo
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_214466 Copy
Species: Homo sapiens
Genetic Insert: ARNT shRNA
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLKO-TET-ON-addgene#21915; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37620297
Proper citation: RRID:Addgene_166910 Copy
Species: Homo sapiens
Genetic Insert: SRRD
Vector Backbone Description: Vector Backbone:pLEX_307; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38315730
Proper citation: RRID:Addgene_214919 Copy
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