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Species: Synthetic
Genetic Insert: DRD2-V2tail-TevN-BLITz1-TetR-VP16
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369042
Proper citation: RRID:Addgene_89874 Copy
Species: Synthetic
Genetic Insert: site-specific DNA-methyltransferase SssI
Vector Backbone Description: Backbone Size:14759; Vector Backbone:pLV hUbC-dCas9-T2A-GFP (#53191); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28695892
Proper citation: RRID:Addgene_89793 Copy
Species: Synthetic
Genetic Insert: LSSmCherry1
Vector Backbone Description: Vector Backbone:pBAD His B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28241009
Proper citation: RRID:Addgene_89986 Copy
Species: Synthetic
Genetic Insert: dCas9-MC
Vector Backbone Description: Backbone Size:4156; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28751638
Comments: Does not contain active methylatransferase - general cloning strains can be used.
Proper citation: RRID:Addgene_89931 Copy
Species: Synthetic
Genetic Insert: LRhR
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: E. coli LacI DNA binding domain, E. coli RbsR linker region, and RbsR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90054 Copy
Species: Synthetic
Genetic Insert: LLhT/V52A
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: E. coli LacI DNA binding domain, LacI linker region, and E. coli TreR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90050 Copy
Species: Synthetic
Genetic Insert: LGhG
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19818797
Comments: E. coli LacI DNA binding domain, E. coli GalR linker region, and GalR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli. The GalR insert contains an E230K mutation compared to wild type.
Proper citation: RRID:Addgene_90048 Copy
Species: Synthetic
Genetic Insert: LPhP
Vector Backbone Description: Backbone Marker:PMID: 3012611; Vector Backbone:pHG165; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19818797
Comments: E. coli LacI DNA binding domain, E. coli PurR linker region, and PurR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90047 Copy
Species: Synthetic
Genetic Insert: LLhC
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: E. coli LacI DNA binding domain, LacI linker region, and E. coli CytR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90046 Copy
Species: Synthetic
Genetic Insert: LGhP
Vector Backbone Description: Backbone Marker:PMID: 3012611; Vector Backbone:pHG165; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24386217
Comments: E. coli LacI DNA binding domain, E. coli GalR linker region, and E. coli PurR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90065 Copy
Species: Synthetic
Genetic Insert: LLhE_AW
Vector Backbone Description: Backbone Marker:PMID: 3012611; Vector Backbone:pHG165; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: E. coli LacI DNA binding domain, LacI linker region, and T. fusca CelR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90062 Copy
Species: Synthetic
Genetic Insert: LEhE
Vector Backbone Description: Backbone Marker:PMID: 3012611; Vector Backbone:pHG165; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: E. coli LacI DNA binding domain, T. fusca CelR linker region, and CelR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90061 Copy
Species: Synthetic
Genetic Insert: DEL
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: LLhG coding region on pHG165a interrupted with a single base-pair deletion . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90064 Copy
Species: Synthetic
Genetic Insert: LLhP
Vector Backbone Description: Backbone Marker:PMID: 3012611; Vector Backbone:pHG165; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17436321
Comments: Second reference: PMID: 22965134. E. coli LacI DNA binding domain, LacI linker region, and E. coli PurR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90038 Copy
Species: Synthetic
Genetic Insert: TagGFP2-TEV-GGG-ELP(120nm)-Cys
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5219; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: The underlying cloning procedure of the employed ELP genes is described in this article:
Ott, W.; Nicolaus, T.; Gaub, H. E.; Nash, M. A. Sequence-Independent Cloning and Post-Translational Modification of Repetitive Protein Polymers through Sortase and Sfp-Mediated Enzymatic Ligation. Biomacromolecules 2016, 17, 1330−1338.
Proper citation: RRID:Addgene_90473 Copy
Species: Synthetic
Genetic Insert: Cys-ELP(120nm)-LPETGG
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28591514
Comments: The underlying cloning procedure of the employed ELP genes is described in this article:
Ott, W.; Nicolaus, T.; Gaub, H. E.; Nash, M. A. Sequence-Independent Cloning and Post-Translational Modification of Repetitive Protein Polymers through Sortase and Sfp-Mediated Enzymatic Ligation. Biomacromolecules 2016, 17, 1330−1338.
Proper citation: RRID:Addgene_90472 Copy
Species: Synthetic
Genetic Insert: TagGFP2-TEV-GGG-ELP(40nm)-Cys
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: The underlying cloning procedure of the employed ELP genes is described in this article:
Ott, W.; Nicolaus, T.; Gaub, H. E.; Nash, M. A. Sequence-Independent Cloning and Post-Translational Modification of Repetitive Protein Polymers through Sortase and Sfp-Mediated Enzymatic Ligation. Biomacromolecules 2016, 17, 1330−1338.
Proper citation: RRID:Addgene_90474 Copy
Species: Synthetic
Genetic Insert: pZ-YFP
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pNH603; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Proper citation: RRID:Addgene_90409 Copy
Species: Synthetic
Genetic Insert: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91029 Copy
Species: Synthetic
Genetic Insert: Csy4-P2A-AtCas9_dead (D10A + H840A)
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91024 Copy
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