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Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18069 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: α-COPI-WD40-Asp115Ala
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35136165
Proper citation: RRID:Addgene_180760 Copy
Genetic Insert: SARS-CoV-2-S2P-B.1.1.529
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.12.29.474491v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181695 Copy
Genetic Insert: SARS-CoV-2-NTD-B.1.1.529
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.12.29.474491v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181698 Copy
Species: Homo sapiens
Genetic Insert: BTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32437509
Proper citation: RRID:Addgene_180806 Copy
Genetic Insert: Phleo/V5
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11254965
Proper citation: RRID:Addgene_1809 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18081 Copy
Species: Synthetic
Genetic Insert: mitoZFD for ND2 mutation
Vector Backbone Description: Backbone Size:5382; Vector Backbone:p3s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35042880
Proper citation: RRID:Addgene_180770 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18082 Copy
Species: evolved fluorescent protein
Genetic Insert: mAmetrine1.1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad/His B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18425137
Comments: Improved photostability (~1.5x better) compared to mAmetrine (Addgene plasmid 18083)
Proper citation: RRID:Addgene_18084 Copy
Genetic Insert: BLA/BB2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11254965
Proper citation: RRID:Addgene_1807 Copy
Genetic Insert: Phleo/BB2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11254965
Proper citation: RRID:Addgene_1808 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18077 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Note that the T4 Lysozyme insert sequence provided by the depositing laboratory differs from GenBank reference sequence NP_049736.1 at two amino acids: R12G and I137R. These differences were confirmed by Addgene's sequencing results.
Proper citation: RRID:Addgene_18110 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18111 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18079 Copy
Species: Mus musculus
Genetic Insert: Inhibitor of differentiation 1
Vector Backbone Description: Backbone Marker:Generated from multiple sources; Backbone Size:6633; Vector Backbone:N/A; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18378907
Proper citation: RRID:Addgene_18112 Copy
Species: Homo sapiens
Genetic Insert: h-RasV12G
Vector Backbone Description: Backbone Marker:Constructed from multiple sources; Backbone Size:6664; Vector Backbone:N/A; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18378907
Proper citation: RRID:Addgene_18113 Copy
Species: SARS-CoV-2
Genetic Insert: S-protein B.1.1.529
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_180774 Copy
Species: Homo sapiens
Genetic Insert: Raptor
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18439900
Comments: Addgene #1859 was PCR amplified with primers containing EcoRI and XhoI, then ligated into pBABE-hygro and fully sequenced
Proper citation: RRID:Addgene_18116 Copy
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