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Species: Homo sapiens
Genetic Insert: HOXC6
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3-HISA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15637592
Proper citation: RRID:Addgene_110366 Copy
Species: Homo sapiens
Genetic Insert: GLS glutaminase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6278; Vector Backbone:psiCHECK2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_110401 Copy
Species: Homo sapiens
Genetic Insert: GLS glutaminase
Vector Backbone Description: Backbone Marker:#50005; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Homology arm for C-Terminal knock-in at stop codon of GLS' KGA isoform. Just mKO2 knock-in.
Construction order:
GLS 1kb 5' Homology Arm: SacI-BamHI;
GLS 1kb 3' Homology Arm BamHI-SalI;
BglII-BamHI mKO2
System options:
sgRNA:
pX330.puro sgRNA KGA Stop1 (Addgene #110405)
Homology arms:
pUC19 KGA HA5'-mKO2-3' (Addgene #110406)
pUC19 KGA HA5'-mKO2-P2A-3' (Addgene #110407)
pUC19 KGA HA5'-mKO2-P2A-Zeo-3' (Addgene #110408)
Proper citation: RRID:Addgene_110406 Copy
Species: Homo sapiens
Genetic Insert: GLS glutaminase
Vector Backbone Description: Backbone Marker:#110403; Backbone Size:9906; Vector Backbone:pX330.puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: sgRNA for GLS exon 19 (KGA isoform stop codon), intended to perform C-Terminal knock-in using pX330 with puromycin resistance.
System options:
sgRNA:
pX330.puro sgRNA KGA Stop1 (Addgene #110405)
Homology arms:
pUC19 KGA HA5'-mKO2-3' (Addgene #110406)
pUC19 KGA HA5'-mKO2-P2A-3' (Addgene #110407)
pUC19 KGA HA5'-mKO2-P2A-Zeo-3' (Addgene #110408)
Proper citation: RRID:Addgene_110405 Copy
Species: Homo sapiens
Genetic Insert: PKCZ
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110512 Copy
Species: Homo sapiens
Genetic Insert: PLEKHS1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110510 Copy
Species: Homo sapiens
Genetic Insert: KGA glutaminase
Vector Backbone Description: Backbone Marker:#1766; Backbone Size:4902; Vector Backbone:pBABE-zeo (pBABE-bleo); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.
Proper citation: RRID:Addgene_110357 Copy
Species: Homo sapiens
Genetic Insert: NDP52
Vector Backbone Description: Backbone Size:5747; Vector Backbone:pBMN-mEGFP-C1; Vector Types:Mammalian Expression, Retroviral, retrovirus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26266977
Proper citation: RRID:Addgene_110465 Copy
Species: Homo sapiens
Genetic Insert: ELAV like RNA binding protein 1
Vector Backbone Description: Backbone Marker:Plasmid #1764; Backbone Size:5177; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.
Proper citation: RRID:Addgene_110346 Copy
Species: Homo sapiens
Genetic Insert: Rab13
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22674975
Proper citation: RRID:Addgene_110500 Copy
Species: Homo sapiens
Genetic Insert: CCDC32
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110505 Copy
Species: Homo sapiens
Genetic Insert: Rab5C-S35N
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22674975
Proper citation: RRID:Addgene_110504 Copy
Species: Homo sapiens
Genetic Insert: PLEKHS1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110509 Copy
Species: Homo sapiens
Genetic Insert: IQCF2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110507 Copy
Species: Homo sapiens
Genetic Insert: Paf1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15632063
Comments: Author has deposited insert sequence. Click on "sequence" to view. Cloned using the following primers 5' CGGGATCCATGGCGCCCACCATCCAG 3' and 5' CCGCTCGAGTCAGTCACTGTCACTATCAGCTTCACTG 3'
Proper citation: RRID:Addgene_11059 Copy
Species: Homo sapiens
Genetic Insert: HRPT2 L64P
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15632063
Comments: Author has deposited insert sequence. Click on "sequence" to view. Used plasmid 11048 as a template for mutagenesis using the following primers: 5' G GAT TCC ATT TTA TTT CTA CCT AAT AAC GTG CAC CTT TCT C 3' and 5' G AGA AAG GAG CAC GTT ATT AGG TAG AAA TAA AAT GGA ATC C 3'
Proper citation: RRID:Addgene_11052 Copy
Species: Homo sapiens
Genetic Insert: Rtf1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15632063
Comments: Author has deposited insert sequence. Click on "sequence" to view. Cloned using the following primers 5' CCCAAGCTTATGAAGAAACAGGCCAACA 3' and 5' CCGCTCGAGAATAAGCCCTCGTCGTTT 3'
Proper citation: RRID:Addgene_11060 Copy
Species: Homo sapiens
Genetic Insert: PCDHB10
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110516 Copy
Species: Homo sapiens
Genetic Insert: SFXN4
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:5600; Vector Backbone:pMXs-IRES-Blasticidin; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30442778
Proper citation: RRID:Addgene_110638 Copy
Species: Homo sapiens
Genetic Insert: SFXN2
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:5600; Vector Backbone:pMXs-IRES-Blasticidin; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30442778
Proper citation: RRID:Addgene_110636 Copy
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