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Species: Homo sapiens
Genetic Insert: Raptor
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18439900
Comments: Addgene #1859 was mutated at two serine residues, then PCR amplified with primers containing EcoRI and XhoI, then ligated into pBABE-hygro and fully sequenced
Proper citation: RRID:Addgene_18117 Copy
Species: Homo sapiens
Genetic Insert: PD-1 (BTLA ITIM)
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34734802
Proper citation: RRID:Addgene_180817 Copy
Species: Homo sapiens
Genetic Insert: BTLA (PD-1 ITIM)
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34734802
Proper citation: RRID:Addgene_180818 Copy
Species: Homo sapiens
Genetic Insert: c-MYC
Vector Backbone Description: Backbone Marker:Dr. Robert Hawley of George Washington University; Backbone Size:5660; Vector Backbone:MSCV IRES GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: The MSCV vector is described in; Hawley, R.G., Lieu, F.H.L., Fong, A.Z.C., and Hawley, T.S. Versatile retroviral vectors for potential use in gene therapy. Gene Ther. 1: 136-138, 1994.
Use of the construct is described in; Park, I., Zhao, R., West, J.A., Yabuuchi, A., Huo, H., Ince, T.A., Lerou, P.H., Lensch, M.W., and Daley, G.Q. Reprogramming of human somatic cells to pluripotency with defined factors. Nature, 451, 141-146, 2008.
Please note that the assembled sequence presented here is not 100% reflective of the actual sequence. See the Addgene diagnostic digest (found under the Resource Information section of the plasmid page) for additional information on the expected plasmid size. Addgene has found another HindIII site at the end of the MYC gene (see our quality control sequence).
Proper citation: RRID:Addgene_18119 Copy
Species: Homo sapiens
Genetic Insert: PD-1
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34734802
Proper citation: RRID:Addgene_180819 Copy
Species: Homo sapiens
Genetic Insert: PD-1int (AA194-288, Y223F, Y248F)
Vector Backbone Description: Vector Backbone:pGEX-6p-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32437509
Proper citation: RRID:Addgene_180815 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: α-COPI-WD40-Arg57Ala
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35136165
Proper citation: RRID:Addgene_180759 Copy
Species: Mus musculus
Genetic Insert: PHR-mCherry-nE-mHP1alpha-delCTE
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3958; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Comments: For additional reference on HP1alpha, please see Larson et al. 2017 Nature, PMID 28636604, https://doi.org/10.1038/nature22822
Proper citation: RRID:Addgene_181891 Copy
Species: Mus musculus
Genetic Insert: RGG-GFP-RGG
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Comments: PCR for RGG-GFP-RGG on Addgene plasmid (#124939),cloned into GFP-mHP1a using AgeI (BshTI) & BspEI (Kpn2I) --> cut out GFP, insert RGG-GFP-RGG
Proper citation: RRID:Addgene_181898 Copy
Species: Homo sapiens
Genetic Insert: FUSN-mHP1alpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Comments: PCR for FUSN on Addgene plasmid (#122148)cloned into GFP-mHP1a using BsrGI (Bsp1407I) & BspEI (Kpn2I)
Proper citation: RRID:Addgene_181897 Copy
Species: Mus musculus
Genetic Insert: PHR-mCherry-PGL-1-mHP1alpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3958; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Proper citation: RRID:Addgene_181894 Copy
Species: E. coli
Genetic Insert: LetB (a.k.a. b1834, yebT, ECK1833)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_181818 Copy
Species: E. coli
Genetic Insert: LetB (a.k.a. b1834, yebT, ECK1833)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_181819 Copy
Species: Synthetic
Genetic Insert: Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36593413
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.07.475005v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181781 Copy
Species: E. coli
Genetic Insert: LetB (a.k.a. b1834, yebT, ECK1833)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_181820 Copy
Genetic Insert: SARS-CoV-2-RBD-SD1-C.37
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.12.29.474491v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181702 Copy
Species: Synthetic
Genetic Insert: mNeonGreen; tetR
Vector Backbone Description: Backbone Size:1706; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35994658
Proper citation: RRID:Addgene_181944 Copy
Species: Synthetic
Genetic Insert: ShTnsB, ShTniQ, ShCas12k-ShTnsC
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36593413
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.07.475005v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181789 Copy
Species: Synthetic
Genetic Insert: nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36593413
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.07.475005v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181784 Copy
Species: Synthetic
Genetic Insert: AcTnsB, AcTnsC, AcTniQ, AcCas12k
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36593413
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.07.475005v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181785 Copy
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