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Vector Backbone Description: Backbone Size:6742; Vector Backbone:pPZP200; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: Contains the 564bp bialaphos resistance (bar) gene from Streptomyces hygroscopicus
Proper citation: RRID:Addgene_134770 Copy
Species: Synthetic
Genetic Insert: SponGee-Kras
Vector Backbone Description: Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31242429
Proper citation: RRID:Addgene_134777 Copy
Species: Synthetic
Genetic Insert: ThPDE5VV
Vector Backbone Description: Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31242429
Proper citation: RRID:Addgene_134778 Copy
Species: Escherichia coli
Genetic Insert: Dihydrofolate reductase
Vector Backbone Description: Backbone Marker:Novagen (EMD Millipore); Backbone Size:4008; Vector Backbone:pACYC-Duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32701056
Proper citation: RRID:Addgene_134817 Copy
Species: humanized S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9
Vector Backbone Description: Backbone Marker:Kuehn lab; Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene 64324), modification of pX330 (Addgene 42230); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597252
Proper citation: RRID:Addgene_135012 Copy
Species: humanized S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9
Vector Backbone Description: Backbone Marker:Kuehn lab; Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene 64324), modification of pX330 (Addgene 42230); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597252
Proper citation: RRID:Addgene_135013 Copy
Species: humanized S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9
Vector Backbone Description: Backbone Marker:Kuehn lab; Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene 64324), modification of pX330 (Addgene 42230); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597252
Proper citation: RRID:Addgene_135014 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9-P2A-mNeonGreen
Vector Backbone Description: Backbone Size:7926; Vector Backbone:L40C; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31735627
Comments: pL40C_PGKintron_Cas9_Green was derived by subcloning the hPGK promoter with beta-globin intron from Tet-pLKO-puro (Addgene #21915) into the L40C-CRISPR.EFS.mNeon.NL.SL vector (a gift from Dirk Heckl, Martin Luther University Halle-Wittenberg) using PacI and BamHI sites.
Proper citation: RRID:Addgene_134966 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:8572; Vector Backbone:pCCL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887975
Proper citation: RRID:Addgene_134984 Copy
Species: Homo sapiens
Genetic Insert: Myo 19
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4713; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30111583
Comments: this article is published:
Stefanie J. Oeding, Katarzyna Majstrowicz, Xiao-Ping Hu, Vera Schwarz, Angelika Freitag, Ulrike Honnert, Petra Nikolaus, Martin Bähler
Journal of Cell Science 2018 131: jcs219469 doi: 10.1242/jcs.219469 Published 10 September 2018
The Myo 19 cDNA was obtained from NITE Biological Resource Center (NBRC), Japan.
cDNA clone AK304073/FLJ61052/TRACH3006685
Proper citation: RRID:Addgene_134988 Copy
Species: Homo sapiens
Genetic Insert: SMURF2
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11163210
Proper citation: RRID:Addgene_13504 Copy
Species: Rattus norvegicus
Genetic Insert: Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4686; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9348541
Comments: See also:
Van den Boom et al.
Molecular Biology of the Cell Vol. 18, No. 4, 1507-1518, April 2007
The Myosin IXb Motor Activity Targets the Myosin IXb RhoGAP Domain as Cargo to Sites of Actin Polymerization.
Published Online:21 Feb 2007https://doi.org/10.1091/mbc.e06-08-0771
Please note: Plasmid contains a T721I mutation in Myo9b. This mutation is not known to affect protein function.
Proper citation: RRID:Addgene_134911 Copy
Vector Backbone Description: Backbone Size:5184; Vector Backbone:BLADE; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30604748
Proper citation: RRID:Addgene_134912 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30604748
Proper citation: RRID:Addgene_134914 Copy
Species: E. coli
Genetic Insert: Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
Vector Backbone Description: Backbone Marker:Mashimo Lab at the Univiersity of Tokyo; Vector Backbone:Synthesized; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31811138
Proper citation: RRID:Addgene_134919 Copy
Species: E. coli
Genetic Insert: Cas3 with bpNLS
Vector Backbone Description: Backbone Marker:Wellcome Sanger Institute; Vector Backbone:pPB-CAG-EBNXN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31811138
Proper citation: RRID:Addgene_134920 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pBBR1MCS-2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34333160
Comments: Plasmid parts were assembled using Golden gate method
Proper citation: RRID:Addgene_134888 Copy
Species: E. coli
Genetic Insert: crRNA for CRISPR-Cas3
Vector Backbone Description: Vector Backbone:pBSIIKS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31811138
Proper citation: RRID:Addgene_134921 Copy
Species: Homo sapiens
Genetic Insert: Rap1A, mCerulean3, RaIGDS RBD, YPet
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31444239
Proper citation: RRID:Addgene_134926 Copy
Species: Homo sapiens
Genetic Insert: Rap1B, Cerulean
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pXFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31444239
Proper citation: RRID:Addgene_134928 Copy
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