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Species: Synthetic
Genetic Insert: Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
Vector Backbone Description: Vector Backbone:pTRANS_230d; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91140 Copy
Species: Synthetic
Genetic Insert: Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
Vector Backbone Description: Vector Backbone:pTRANS_220d; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91136 Copy
Species: Synthetic
Genetic Insert: Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
Vector Backbone Description: Vector Backbone:pTRANS_220d; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91135 Copy
Species: Synthetic
Genetic Insert: Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
Vector Backbone Description: Vector Backbone:pTRANS_210d; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91130 Copy
Species: Synthetic
Genetic Insert: Csy4 (+ BaeI site for GT donor cloning)
Vector Backbone Description: Vector Backbone:pMOD_C0000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91091 Copy
Species: Synthetic
Genetic Insert: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
Vector Backbone Description: Vector Backbone:pMOD_C0000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91090 Copy
Species: Synthetic
Genetic Insert: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91049 Copy
Species: Synthetic
Genetic Insert: TREX2-P2A-TaCas9
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91041 Copy
Species: Synthetic
Genetic Insert: Engineering Reagent: ZmUbi:TALEN
Vector Backbone Description: Vector Backbone:pDIRECT_23J; Vector Types:Plant Expression, TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91152 Copy
Species: Synthetic
Genetic Insert: Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
Vector Backbone Description: Vector Backbone:pTRANS_260d; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91150 Copy
Species: Synthetic
Genetic Insert: BaeI LacZ cassette for GT donor cloning
Vector Backbone Description: Vector Backbone:pMOD_C0000; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91103 Copy
Species: Synthetic
Genetic Insert: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
Vector Backbone Description: Vector Backbone:pMOD_B2000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91069 Copy
Species: Synthetic
Genetic Insert: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
Vector Backbone Description: Vector Backbone:pMOD_B2000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Comments: Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers - combinable with another array of multiple gRNA spacers in MODULE C, Terminator: none (will be fused to gRNA array in MODULE C)
Proper citation: RRID:Addgene_91066 Copy
Species: Synthetic
Genetic Insert: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
Vector Backbone Description: Vector Backbone:pMOD_B2000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91067 Copy
Species: Synthetic
Genetic Insert: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
Vector Backbone Description: Vector Backbone:pMOD_B2000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91060 Copy
Species: Synthetic
Genetic Insert: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
Vector Backbone Description: Vector Backbone:pMOD_B2000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91061 Copy
Species: Synthetic
Genetic Insert: dTaCas9-VPR
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91054 Copy
Species: Synthetic
Genetic Insert: dAtCas9-VP64
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91051 Copy
Species: Synthetic
Genetic Insert: Csy4-P2A-dAtCas9-VPR
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91052 Copy
Species: Synthetic
Genetic Insert: dAtCas9-VPR
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91050 Copy
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