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Species: Rattus norvegicus
Genetic Insert: Rat Fibronectin EIIIB
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2869; Vector Backbone:pGEM-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3528152
Proper citation: RRID:Addgene_14060 Copy
Species: oriza sativa
Genetic Insert: OsTIR1 mutated F74A
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBlueScript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32941625
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.01.20.912113v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_140615 Copy
Species: oriza sativa
Genetic Insert: OsTIR1 mutated F74A
Vector Backbone Description: Backbone Size:5836; Vector Backbone:pEGFP-c1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32941625
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.01.20.912113v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_140616 Copy
Species: oriza sativa
Genetic Insert: OsTIR1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBlueScript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32941625
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.01.20.912113v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_140617 Copy
Species: Other
Genetic Insert: sgRNA for pAID-EF1a plasmid digestion
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32941625
Comments: pX330 plasmid was obtained from Addgene (#42230).
Please visit https://www.biorxiv.org/content/10.1101/2020.01.20.912113v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_140610 Copy
Species: Synthetic
Genetic Insert: pegRNA EMX1 +38
Vector Backbone Description: Backbone Marker:Anzalone et al.; Vector Backbone:pU6-pegRNA; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32187529
Proper citation: RRID:Addgene_140579 Copy
Species: Synthetic
Genetic Insert: gRNA RNF103-CHMP3
Vector Backbone Description: Vector Backbone:pmCherry-U6; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32187529
Proper citation: RRID:Addgene_140584 Copy
Vector Backbone Description: Vector Backbone:pmCherry-U6; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32187529
Proper citation: RRID:Addgene_140580 Copy
Species: Synthetic
Genetic Insert: gRNA TTLL11
Vector Backbone Description: Vector Backbone:pmCherry-U6; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32187529
Proper citation: RRID:Addgene_140582 Copy
Species: Synthetic
Genetic Insert: gRNA CLIC3
Vector Backbone Description: Vector Backbone:pmCherry-U6; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32187529
Proper citation: RRID:Addgene_140583 Copy
Species: Vibrio cholera, Tatumella citrea
Genetic Insert: Mini-Tn6677, T7lac-GDH gene cassette
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32551502
Proper citation: RRID:Addgene_140629 Copy
Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32551502
Comments: An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140625 Copy
Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-tra13
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32551502
Comments: An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140628 Copy
Species: Synthetic
Genetic Insert: gRNA VEGFA
Vector Backbone Description: Vector Backbone:pmCherry-U6; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32187529
Proper citation: RRID:Addgene_140588 Copy
Species: Synthetic
Genetic Insert: gRNA ALDH1A3
Vector Backbone Description: Vector Backbone:pmCherry-U6; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32187529
Proper citation: RRID:Addgene_140589 Copy
Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32551502
Comments: An unexpected 65 bp insertion was detected in the origin of replication, compared to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140622 Copy
Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32551502
Comments: An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140624 Copy
Species: Scytonema hofmanni
Genetic Insert: ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32551502
Proper citation: RRID:Addgene_140631 Copy
Species: Homo sapiens
Genetic Insert: SLC25A51 with 5' and 3' UTR of scNDT1
Vector Backbone Description: Vector Backbone:YCplac33C; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33087354
Proper citation: RRID:Addgene_140591 Copy
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:CRISPRi-ME (Jeffrey Barrick Laboratory); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32246719
Comments: Derivative of CRISPRi-ME from Peng et al., "Synthetic Genome Defenses against Selfish DNA Elements Stabilize Engineered Bacteria against Evolutionary Failure", ACS Synthetic Biology, 2019. Vector was modified to take the dCas9 to Cas9 and gRNA was substituted to no longer target the insertion sequences, but instead the pcaHG genes in ADP1.
Proper citation: RRID:Addgene_140637 Copy
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