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Species: Homo sapiens
Genetic Insert: SFXN5
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:5600; Vector Backbone:pMXs-IRES-Blasticidin; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30442778
Proper citation: RRID:Addgene_110639 Copy
Species: Homo sapiens
Genetic Insert: St1Cas9 LMD-9
Vector Backbone Description: Vector Backbone:pX602 (Plasmid #61593); Vector Types:Mammalian Expression, Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31900288
Comments: Mammalian codon-optimized Streptococcus thermophilus CRISPR1-Cas9 LMD-9 was subcloned from MSP1594 (Plasmid #65775). An SV40 NLS was added to the N-terminus. Please visit https://www.biorxiv.org/content/early/2018/05/14/321208 for BioRxiv preprint
Proper citation: RRID:Addgene_110624 Copy
Species: Homo sapiens
Genetic Insert: St1Cas9 LMD-9
Vector Backbone Description: Vector Backbone:MSP1594 (Plasmid #65775); Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31900288
Comments: This plasmid is identical to MSP1594 (Plasmid #65775) except for the addition of an SV40 NLS to the N-terminus of mammalian codon-optimized Streptococcus thermophilus CRISPR1-Cas9 LMD-9. Please visit https://www.biorxiv.org/content/early/2018/05/14/321208 for BioRxiv preprint
Proper citation: RRID:Addgene_110625 Copy
Species: Homo sapiens
Genetic Insert: BRAFV600E IRES H2B-mOrange2
Vector Backbone Description: Vector Backbone:pHIV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29990501
Proper citation: RRID:Addgene_110732 Copy
Species: Homo sapiens
Genetic Insert: p14ARF IRES H2B-mOrange2
Vector Backbone Description: Vector Backbone:pHIV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29990501
Comments: Addgene sequencing detected a potentially mixed population, with a C to T nucleotide change that would cause a T8I mutation within the CDKN2A insert. Please screen multiple clones to ensure that the correct (C) one is isolated before proceeding with downstream work.
Proper citation: RRID:Addgene_110731 Copy
Species: Homo sapiens
Genetic Insert: CDKN2A gRNA
Vector Backbone Description: Vector Backbone:pEN35; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29990501
Proper citation: RRID:Addgene_110736 Copy
Species: Homo sapiens
Genetic Insert: CDKN2A gRNA
Vector Backbone Description: Vector Backbone:pEN35; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29990501
Proper citation: RRID:Addgene_110737 Copy
Species: Homo sapiens
Genetic Insert: H2B-mOrange2
Vector Backbone Description: Vector Backbone:pHDR; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29990501
Proper citation: RRID:Addgene_110735 Copy
Species: Homo sapiens
Genetic Insert: Pro-SBP-mGFP-COL1A1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30587510
Comments: See attached PDF for full descritions
Proper citation: RRID:Addgene_110726 Copy
Species: Homo sapiens
Genetic Insert: p14ARF IRES zsGreen
Vector Backbone Description: Vector Backbone:pHIV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29990501
Proper citation: RRID:Addgene_110729 Copy
Species: Homo sapiens
Genetic Insert: PBZ domain from APLF, amino acids 371-451
Vector Backbone Description: Backbone Marker:Michael Lin, Addgene plasmid #40260; Vector Backbone:pcDNA3-mRuby2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29789535
Proper citation: RRID:Addgene_110650 Copy
Species: Homo sapiens
Genetic Insert: mClover-AID-P2A-CD5cmyc2-bglpA
Vector Backbone Description: Vector Backbone:pUCHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30816313
Proper citation: RRID:Addgene_110656 Copy
Species: Homo sapiens
Genetic Insert: mClover-AID-P2A-CD5Flag2-bglpA
Vector Backbone Description: Vector Backbone:pUCHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30816313
Proper citation: RRID:Addgene_110654 Copy
Species: Homo sapiens
Genetic Insert: CD5Flag2-P2A-AID-mClover
Vector Backbone Description: Vector Backbone:pUCHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30816313
Proper citation: RRID:Addgene_110657 Copy
Species: Homo sapiens
Genetic Insert: CD5HA2-P2A-AID-mClover
Vector Backbone Description: Vector Backbone:pUCHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30816313
Proper citation: RRID:Addgene_110658 Copy
Species: Homo sapiens
Genetic Insert: CMV-CDcmyc2-bglpA
Vector Backbone Description: Vector Backbone:pJet 1.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30816313
Proper citation: RRID:Addgene_110645 Copy
Species: Homo sapiens
Genetic Insert: PBZ domain from APLF, amino acids 371-451
Vector Backbone Description: Backbone Marker:Chang-Deng Hu, Addgene plasmid #22011; Vector Backbone:pBiFC-VC155; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29789535
Proper citation: RRID:Addgene_110648 Copy
Species: Homo sapiens
Genetic Insert: ISG15
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29463763
Proper citation: RRID:Addgene_110761 Copy
Species: Homo sapiens
Genetic Insert: ISG15
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29463763
Proper citation: RRID:Addgene_110760 Copy
Species: Homo sapiens
Genetic Insert: UBB
Vector Backbone Description: Backbone Marker:Merck; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29463763
Proper citation: RRID:Addgene_110766 Copy
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