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Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:CRISPRi-ME (Jeffrey Barrick Laboratory); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32246719
Comments: Derivative of CRISPRi-ME from Peng et al., "Synthetic Genome Defenses against Selfish DNA Elements Stabilize Engineered Bacteria against Evolutionary Failure", ACS Synthetic Biology, 2019. Vector was modified to take the dCas9 to Cas9 and gRNA was substituted to no longer target the insertion sequences, but instead the vanAB genes in ADP1.
Proper citation: RRID:Addgene_140638 Copy
Species: Danio rerio
Genetic Insert: hsp70l
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3156; Vector Backbone:pDEST; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31888978
Proper citation: RRID:Addgene_140639 Copy
Species: Homo sapiens
Genetic Insert: CTCF
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140647 Copy
Species: Homo sapiens
Genetic Insert: SMC2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140648 Copy
Genetic Insert: Ach sensor V9
Vector Backbone Description: Backbone Size:9951; Vector Backbone:pSinREP5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_140643 Copy
Genetic Insert: Ach sensor V9
Vector Backbone Description: Vector Backbone:plenti-hysynpasin-vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_140644 Copy
Species: Homo sapiens
Genetic Insert: BRD4
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140650 Copy
Species: Homo sapiens
Genetic Insert: TOP2A
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140652 Copy
Species: Homo sapiens
Genetic Insert: OsTIR1(WT) mAID-EGFP-Nluc TOL2
Vector Backbone Description: Vector Backbone:pTSAL200R175-CAGGS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140658 Copy
Species: Homo sapiens
Genetic Insert: TOP2A
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140654 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OsTIR1(WT)
Vector Backbone Description: Vector Backbone:pRS306-GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140655 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL-OsTIR1(F74G)
Vector Backbone Description: Vector Backbone:pRS306-GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140656 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL-OsTIR1(F74A)
Vector Backbone Description: Vector Backbone:pRS306-GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140657 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosphila Beta Nu Integrin
Vector Backbone Description: Backbone Size:3000; Vector Backbone:PBluescript II SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8076521
Proper citation: RRID:Addgene_14069 Copy
Genetic Insert: rtTAM2
Vector Backbone Description: Vector Backbone:pPB-MCSfw-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29129686
Proper citation: RRID:Addgene_140827 Copy
Genetic Insert: cR control for toggle switch experiment
Vector Backbone Description: Vector Backbone:pCOLA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32488086
Comments: GenBank file with original annotations can be downloaded from the "Resource Information" section. Please note that, in addition, plasmids pJ2018 and pJ2077.2 are needed for the circuit encoded on this plasmid to operate as designed.
Proper citation: RRID:Addgene_140669 Copy
Species: Synthetic
Genetic Insert: 24xMS2V7
Vector Backbone Description: Backbone Marker:Genescript; Backbone Size:2749; Vector Backbone:pUC57; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131164
Proper citation: RRID:Addgene_140705 Copy
Species: Homo sapiens
Genetic Insert: OCT4
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pTRE-Tight-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29129686
Comments: Depositor confirms the L104P mutation in KLF2 does not affect plasmid function.
Proper citation: RRID:Addgene_140826 Copy
Genetic Insert: NBαMs/Rt-IgG1-Fc
Vector Backbone Description: Vector Backbone:pMal-T-Avi-His/BirA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32062022
Proper citation: RRID:Addgene_140700 Copy
Genetic Insert: NBαMs-IgK
Vector Backbone Description: Vector Backbone:pMal-T-Avi-His/BirA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32062022
Proper citation: RRID:Addgene_140701 Copy
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