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Species: S. mediterranea (planarian)
Genetic Insert: npy-6
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26478 Copy
Species: S. mediterranea (planarian)
Genetic Insert: spp-3
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: Addgene's quality control sequence has minor discrepancies with the provided depositor's sequence and NCBI; these do not affect plasmid function.
Proper citation: RRID:Addgene_26510 Copy
Species: S. mediterranea (planarian)
Genetic Insert: spp-6
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Proper citation: RRID:Addgene_26512 Copy
Species: S. mediterranea (planarian)
Genetic Insert: mpl-2
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26471 Copy
Species: E.coli
Genetic Insert: KanamycinResistance-RpsL
Vector Backbone Description: Backbone Size:2935; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:19160076
Proper citation: RRID:Addgene_20871 Copy
Species: Synthetic
Genetic Insert: GLT1 POL1 24xPP7SL
Vector Backbone Description: Vector Backbone:pHIS; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21512033
Comments: Note that Addgene NGS results found 22xPP7 stem loop repeats rather than expected 24. It is not known how this impacts plasmid function.
This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid.
Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert.
Proper citation: RRID:Addgene_35196 Copy
Species: Human Merkel Cell polyomavirus
Genetic Insert: MCV replication region
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:18812503
Proper citation: RRID:Addgene_28192 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rRNA 5'ETS ntds 923 to 1179
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15489292
Proper citation: RRID:Addgene_23995 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 25S rRNA from +5270 to oligo y
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15489292
Proper citation: RRID:Addgene_23996 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rDNA 5'ETS ntds 1 to 177
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15489292
Proper citation: RRID:Addgene_23993 Copy
Species: Gallus gallus
Genetic Insert: DISP1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII-TOPO; Vector Types:cDNA for in situ hybridization; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:16246323
Comments: For riboprobe cut with SpeI. Runoffs with T7 polymerase. This is not full length ORF.
Proper citation: RRID:Addgene_31235 Copy
Species: Mustela putorius furo
Genetic Insert: SLC17A7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII-TOPO; Vector Types:cDNA for in situ hybridization; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20575059
Comments: For riboprobe cut with NotI or EcoRI. Runoffs with SP6.
Proper citation: RRID:Addgene_31225 Copy
Species: Polyomavirus
Genetic Insert: Half genome of WU polyomavirus
Vector Backbone Description: Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:17480120
Proper citation: RRID:Addgene_31494 Copy
Species: polyomavirus
Genetic Insert: Half genome of WU polyomavirus
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3957; Vector Backbone:pCR4-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:17480120
Proper citation: RRID:Addgene_31493 Copy
Vector Backbone Description: Vector Backbone:pSC189; Vector Types:transposon delivery vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:12383515
Proper citation: RRID:Addgene_32114 Copy
Species: Staphylococcus aureus
Genetic Insert: Protein A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_32225 Copy
Genetic Insert: QF Kan
Vector Backbone Description: Vector Backbone:pBSC5; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21857163
Comments: Please see http://www.everyvector.com/sequences/show_public/14110 for additional plasmid map.
Proper citation: RRID:Addgene_32321 Copy
Genetic Insert: ECFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Comments: For the addition of ECFP to the N-terminus of another cDNA by cloning the cDNA into the NotI site (of the original pCR2.1 TOPO).
Proper citation: RRID:Addgene_32436 Copy
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Comments: For the addition of EYFP to the C-terminus of another cDNA by cloning the cDNA into the BamHI site (of the original pCR2.1 TOPO).
Proper citation: RRID:Addgene_32437 Copy
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Comments: For the addition of EYFP to the N-terminus of another cDNA by cloning the cDNA into the NotI site (of the original pCR2.1 TOPO).
Proper citation: RRID:Addgene_32438 Copy
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