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Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223949 Copy
Species: Synthetic
Genetic Insert: 3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
Vector Backbone Description: Backbone Marker:Matxalen Llosa lab; Vector Backbone:pMTX808; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39560384
Comments: RSF1010-based plasmid. Expresses 3xF-HF-BE3 from lacIq-Ptac promoter. Basal expression is sufficient for base editing in Coxiella burnetii. Note: During growth of E. coli harboring this plasmid, in some cells/colonies, the basal expression of 3xF-HF-BE3 can lead to spurious deamination of cytosines on the plasmid.
Unique XhoI, NotI and XbaI sites for addition of sgRNA-encoding region(s) from pHelper-CBE-sgRNA (Addgene plasmid #221137) helper plasmid derivatives. Plasmid harbors oriT, but relaxase gene mobA is interrupted by AmpR. Plasmid alias: pSAST356.
Proper citation: RRID:Addgene_221138 Copy
Species: Synthetic
Genetic Insert: 3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
Vector Backbone Description: Backbone Marker:Matxalen Llosa lab; Vector Backbone:pMTX808; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39560384
Comments: RSF1010-based plasmid. Expresses 3xF-BE4-PpAPOBEC1(H122A) from lacIq-Ptac promoter. IPTG induction is required for base editing in Coxiella burnetii. Unique XhoI, NotI and SapI sites for addition of sgRNA-encoding region(s) from pHelper-CBE-sgRNA (Addgene plasmid #221137) helper plasmid derivatives. Plasmid harbors oriT, but relaxase gene mobA is interrupted by AmpR. Plasmid encodes sacB, but sacB is not required for Coxiella burnetii base editing protocol. Plasmid alias: pSAST372.
Proper citation: RRID:Addgene_221139 Copy
Vector Backbone Description: Backbone Marker:Wellcome Trust Sanger; Vector Backbone:pMCS-AAT-PB:PGKpuro∆tk; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38986614
Comments: Please visit https://doi.org/10.1101/2023.11.03.565112 for bioRxiv preprint.
Proper citation: RRID:Addgene_218893 Copy
Vector Backbone Description: Vector Backbone:pAC1801_pmax-dCasRx (Addgene #118634); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38917795
Comments: This was the destination vector used for generating the dCasRx-SF library for screening by co-transfection with the luciferase reporters. We have found that cloning dCasRx-SFs into the pXR002 backbone gave greater transfection efficiency for endogenous applications. We recommend using the EF1a-dCasRx-RBM25 vector (Addgene #221001) for endogenous targeting.
Proper citation: RRID:Addgene_221003 Copy
Vector Backbone Description: Backbone Marker:Addgene #91798; Backbone Size:9621; Vector Backbone:pLEX_305-C-dTAG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37994369
Proper citation: RRID:Addgene_221465 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Swapped tags from pcDNA3.1-ccdB-3xFLAG-V5 (addgene #87063)
Proper citation: RRID:Addgene_214985 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Swapped tags from pcDNA3.1-ccdB-3xFLAG-V5 (addgene #87063)
Proper citation: RRID:Addgene_214988 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7061; Vector Backbone:pDESTattB; Vector Types:Zebrafish Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38838146
Comments: Please visit https://doi.org/10.1101/2023.12.08.570868 for bioRxiv preprint.
Proper citation: RRID:Addgene_213511 Copy
Vector Backbone Description: Vector Backbone:pMK33; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_196282 Copy
Species: Synthetic
Genetic Insert: PAmCherry-FLAG-cat
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_211832 Copy
Vector Backbone Description: Backbone Marker:ATCC(pUC18) and Christensen and Quail(pAHC25); Backbone Size:4930; Vector Backbone:pUC18 and pAHC25; Vector Types:Plant expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_33297 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: New version of Neomycin resistance vector for drug selection in worms.
This vector replaces pBCN24-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells).
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34919 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms.
This vector contains a Pmyo-2::EGFP::myo-2_3'UTR pharyngeal marker on the backbone. Worm strains generated with vector can be crossed with strains generated with other visual markers such as in pBCN40-R4R3 which has a red fluorescent pharyngeal marker.
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34916 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi14024 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34862 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4391 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: There is a 461 bp deletion in the middle of the left recombination region. This deletion does not appear to adversely affect the ability to insert transgenes into the ttTi4391 site.
Proper citation: RRID:Addgene_34861 Copy
Species: Caenorhabditis elegans
Genetic Insert: cxTi10816 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.
Proper citation: RRID:Addgene_34864 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:12020; Vector Backbone:pBID-UASC-MRG; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of myc tagRFP (tRFP) fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). myc-tRFP is fused to the Nterminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Proper citation: RRID:Addgene_35205 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197841 Copy
Vector Backbone Description: Backbone Size:10157; Vector Backbone:pLEX_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201122 Copy
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