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Species: Other
Genetic Insert: LexA
Vector Backbone Description: Backbone Marker:Koen Venken; Vector Backbone:pBS-KS-attB-1-2-PT-SA-SD-1; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25712101
Proper citation: RRID:Addgene_125221 Copy
Species: Other
Genetic Insert: GeneSwitch
Vector Backbone Description: Backbone Marker:Koen Venken; Vector Backbone:pBS-KS-attB-1-2-PT-SA-SD-1; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25712101
Proper citation: RRID:Addgene_125215 Copy
Species: Other
Genetic Insert: GeneSwitch
Vector Backbone Description: Backbone Marker:Koen Venken; Vector Backbone:pBS-KS-attB-1-2-PT-SA-SD-0; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25712101
Proper citation: RRID:Addgene_125214 Copy
Species: Other
Genetic Insert: optimized RBS
Vector Backbone Description: Backbone Size:3729; Vector Backbone:pFD116 with an optimized RBS to use in E. coli; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31377338
Proper citation: RRID:Addgene_125546 Copy
Species: Other
Genetic Insert: BE4max-NG
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-auFYCwa9cwpOwF75PnyS for additional information.
Proper citation: RRID:Addgene_125617 Copy
Species: Other
Genetic Insert: evoFERNY-BE4max-NG
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-yOkx507aEyhFduGQKQOz for additional information.
Proper citation: RRID:Addgene_125615 Copy
Species: Other
Genetic Insert: evoAPOBEC1-BE4max-NG
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-YMX6CoDtiqAsxyhvf3mx for additional information.
Proper citation: RRID:Addgene_125616 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:25806970
Comments: Please note that an IS1 transposase sequence was found downstream of Cas9 during Addgene's quality control. The depositor noted that this sequence should NOT affect plasmid function.
Proper citation: RRID:Addgene_125688 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spdCas9, sgRNA casette
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:25806970
Proper citation: RRID:Addgene_125687 Copy
Species: Other
Genetic Insert: hChR2(H134R)-eYFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5581; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126080 Copy
Species: Other
Genetic Insert: hChR2(H134R)-eYFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5581; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126081 Copy
Species: Other
Genetic Insert: codon-optimized AcrIIC3Nme
Vector Backbone Description: Backbone Size:4896; Vector Backbone:pCSDest2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31439808
Proper citation: RRID:Addgene_126202 Copy
Species: Other
Genetic Insert: codon-optimized AcrIIC3Nme
Vector Backbone Description: Backbone Size:4954; Vector Backbone:pCSDest2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31439808
Proper citation: RRID:Addgene_126203 Copy
Species: Other
Genetic Insert: intB13 integrase casette
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30853419
Proper citation: RRID:Addgene_120230 Copy
Species: Other
Genetic Insert: sfGFP/mScarletP2A-mcs
Vector Backbone Description: Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that sfGFP can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A
The associated publication utilized mNeon instead of sfGFP/mClover3, but mNeon has is exceedingly difficult to share. Therefore, these two plasmids (120261 and 120262) are made available, using sfGFP and mClover3 instead of mNeon. These plasmids have been tested, and are equivalent to the mNeon variant.
Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_120261 Copy
Species: Other
Genetic Insert: N-terminal fragment of SpyCas9
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120293 Copy
Species: Other
Genetic Insert: C-terminal fragment of SpyCas9
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120294 Copy
Species: Other
Genetic Insert: C-terminal fragment of SpyCas9
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120295 Copy
Species: Other
Genetic Insert: AcrIIA4
Vector Backbone Description: Vector Backbone:scAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982889
Comments: Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
Proper citation: RRID:Addgene_120297 Copy
Species: Other
Genetic Insert: mClover
Vector Backbone Description: Backbone Marker:Epicentre; Vector Backbone:pMOD3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28264909
Comments: D103N mutation in mClover does not affect function.
Proper citation: RRID:Addgene_120330 Copy
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