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Species: Synthetic
Genetic Insert: SUMO
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus_SUMOstar; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40390 Copy
Species: Synthetic
Genetic Insert: GST
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus N-His; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40399 Copy
Species: Synthetic
Genetic Insert: SUMO
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus_SUMOstar; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40395 Copy
Species: Synthetic
Genetic Insert: SUMO
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus_SUMOstar; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40394 Copy
Species: Synthetic
Genetic Insert: SUMO
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus_SUMOstar; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40392 Copy
Species: Synthetic
Genetic Insert: SUMO
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus_SUMOstar; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40388 Copy
Species: Synthetic
Genetic Insert: Aurora B FRET-sensor
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5125; Vector Backbone:pIRES-puro2b; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22801782
Proper citation: RRID:Addgene_40731 Copy
Species: Synthetic
Genetic Insert: Leucine Zipper prime - C super positive Green Fluorescent Protein
Vector Backbone Description: Backbone Size:4496; Vector Backbone:pMRBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22692102
Proper citation: RRID:Addgene_40730 Copy
Species: Synthetic
Genetic Insert: CFP-24xPP7
Vector Backbone Description: Vector Backbone:pHAGE-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22735544
Comments: Please note that the final PP7 stem loop ends with CCAGCAGAGCATATGGGCTCG The depositing laboratory confirmed that the plasmid functions as described in the associated publication.
The sequence for a single cassette (with the two non-identical stem-loops in uppercase) is:
taaggtacctaattgcctagaaaGGAGCAGACGATATGGCGTCGCTCCctgcaggtcgactctagaaa- CCAGCAGAGCATATGGGCTCGCTGGctgcagtattcccgggttcatt
Proper citation: RRID:Addgene_40652 Copy
Species: Synthetic
Genetic Insert: CFP-24xMBS
Vector Backbone Description: Vector Backbone:pHAGE-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22735544
Comments: The Singer Lab no longer recommends this plasmid, as it has a higher rate of bacterial recombination.
For mammalian cells they recommend the following plasmids:
pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre (Plasmid #84561)
HaloTag-bActinCDS-bActinUTR-MS2V5 (Plasmid #102718)
For yeast cells they recommend the following plasmids:
pET246-pUC57 12xMS2V6 (Plasmid #104390)
pET259-pUC57 24xMS2V6 (Plasmid #104391)
pET251-pUC 12xMS2V6 Loxp KANr Loxp (Plasmid #104392)
pET264-pUC 24xMS2V6 Loxp KANr Loxp (Plasmid #104393)
Due to the propensity of the plasmid to recombine, Addgene recommends screening multiple colonies by restriction analysis to ensure that the isolated clones contain a full set of 24 MS2 repeats
Proper citation: RRID:Addgene_40651 Copy
Species: Synthetic
Genetic Insert: tdPCP-gfp
Vector Backbone Description: Vector Backbone:pHAGE-UBC-RIG (modified); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22735544
Comments: The linker region between the two PCPs is CGTGCGGATCCGCTAGCCTCC
Proper citation: RRID:Addgene_40650 Copy
Species: Synthetic
Genetic Insert: tdMCP-gfp
Vector Backbone Description: Vector Backbone:pHAGE-UBC-RIG (modified); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22735544
Comments: The linker region between the two MCPs is ATCTACGCCATGGCTTCT
Proper citation: RRID:Addgene_40649 Copy
Species: Synthetic
Genetic Insert: Cerulean
Vector Backbone Description: Backbone Size:6336; Vector Backbone:PBCAG-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22521325
Proper citation: RRID:Addgene_40997 Copy
Species: Synthetic
Genetic Insert: mRFP
Vector Backbone Description: Backbone Size:6336; Vector Backbone:PBCAG-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22521325
Proper citation: RRID:Addgene_40996 Copy
Species: Synthetic
Genetic Insert: PiggyBac transposon 3' terminal repeats
Vector Backbone Description: Backbone Size:5551; Vector Backbone:pCAGGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22521325
Proper citation: RRID:Addgene_40973 Copy
Species: Synthetic
Genetic Insert: D4 second generation cameleon (calcium sensor)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16720273
Comments: Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and citrine. D4 does not bind wild-type calmodulin. It has a Kd' of 195 microM.
See also Palmer & Tsien (2006) Nature Protocols.
Proper citation: RRID:Addgene_37473 Copy
Species: Synthetic
Genetic Insert: D2 second generation cameleon (calcium sensor)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16720273
Comments: Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and citrine. D2 does not bind wild-type calmodulin. It has a Kd1' and Kd2' of 0.8 microM and 28 microM.
See also Palmer & Tsien (2006) Nature Protocols.
Proper citation: RRID:Addgene_37470 Copy
Species: Synthetic
Genetic Insert: PIGA zinc finger L1
Vector Backbone Description: Backbone Marker:Keith Joung lab @ MGH/HMS; Backbone Size:5709; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Comments: Please also cite the original article from Joung lab describing the construction of zinc finger nuclease:
Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, et al. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 2008;31:294–301.
Proper citation: RRID:Addgene_37797 Copy
Species: Synthetic
Genetic Insert: PIGA zinc finger L2
Vector Backbone Description: Backbone Marker:Keith Joung lab @ MGH/HMS; Backbone Size:5709; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Comments: Please also cite the original article from Joung lab describing the construction of zinc finger nuclease:
Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, et al. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 2008;31:294–301.
Proper citation: RRID:Addgene_37798 Copy
Species: Synthetic
Genetic Insert: PIGA zinc finger R1
Vector Backbone Description: Backbone Marker:Keith Joung lab @ MGH/HMS; Backbone Size:5709; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Comments: Please also cite the original article from Joung lab describing the construction of zinc finger nuclease:
Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, et al. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 2008;31:294–301.
Proper citation: RRID:Addgene_37799 Copy
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