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Species: Synthetic
Genetic Insert: monomeric streptavidin, EGFP, TM helix
Vector Backbone Description: Backbone Marker:Life Technology; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22806584
Proper citation: RRID:Addgene_39863 Copy
Species: Synthetic
Genetic Insert: monomeric streptavidin fused to EGFP
Vector Backbone Description: Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22806584
Proper citation: RRID:Addgene_39862 Copy
Species: Rattus norvegicus
Genetic Insert: TrkB.T1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9927421
Comments: The T1 isoform of trkB was cloned from a pCMV-5 trkB.T1 construct using HindIII and XbaI to fuse it to the HA-tagged N-terminus of trkB in the vector pB-KS followed by subcloning into pRC/CMV AC7 with NheI and NotI.
See Middlemas et al (Mol Cell Biol. 1991 Jan;11(1):143-53., https://www.ncbi.nlm.nih.gov/pubmed?term=1846020 ) to learn more about the TrkB.T1 variant.
Please note that the endogenous signal sequence of TrkB-T1 was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequences for GenBank ID M55292.1 or NM_001163168.1, the TrkB-T1 protein sequence starts at amino acid #32
Proper citation: RRID:Addgene_39980 Copy
Species: Homo sapiens
Genetic Insert: hSlp2-a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40056 Copy
Species: Homo sapiens
Genetic Insert: hSlp2-a C2AB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40051 Copy
Species: Homo sapiens
Genetic Insert: Emi1
Vector Backbone Description: Vector Backbone:pT7-Venus-C1; Vector Types:in vitro transcription; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17485488
Proper citation: RRID:Addgene_39854 Copy
Species: Rattus norvegicus
Genetic Insert: TrkA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9927421
Comments: cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide
Please note that the endogenous signal sequence of TrkA was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to GenBank ID NP_067600.1, the TrkA protein sequence starts at amino acid #30
Proper citation: RRID:Addgene_39977 Copy
Species: Homo sapiens
Genetic Insert: hSlp4-a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pDEST15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40046 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Comments: An alternative version of this plasmid (containing only loxP-Neo-loxP in the intron) is also available from Addgene:
pCA-G-intron(Neo)-T(LNL)
Proper citation: RRID:Addgene_40020 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_40019 Copy
Species: Homo sapiens
Genetic Insert: P85(T576del)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40228 Copy
Species: Homo sapiens
Genetic Insert: P85(KS459delN)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40222 Copy
Species: Rattus norvegicus
Genetic Insert: SAPAP2
Vector Backbone Description: Vector Backbone:pCMV5 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9115257
Proper citation: RRID:Addgene_40216 Copy
Species: Rattus norvegicus
Genetic Insert: S-SCAM
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9694864
Proper citation: RRID:Addgene_40213 Copy
Genetic Insert: Promotor probe
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11059491
Comments: Please note that there is a T=>C mutation, causing LVA=>LAA change in aa.
Proper citation: RRID:Addgene_40171 Copy
Species: Synthetic
Genetic Insert: Erk activity reporter
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:7711; Vector Backbone:pLenti pRRL-SV40(puro)_CMV(mcs); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23882122
Comments: A genetically encoded fluorescent sensor of ERK activity. 2008. PNAS 205(49)
Proper citation: RRID:Addgene_40178 Copy
Species: Homo sapiens
Genetic Insert: Artemis
Vector Backbone Description: Backbone Marker:In-house; Backbone Size:4442; Vector Backbone:pUC; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941364
Proper citation: RRID:Addgene_40211 Copy
Species: Synthetic
Genetic Insert: Erk activity reporter
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4900; Vector Backbone:pTriEx4; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23882122
Comments: A genetically encoded fluorescent sensor of ERK activity. 2008. PNAS 205(49)
Proper citation: RRID:Addgene_40174 Copy
Species: Homo sapiens
Genetic Insert: HIF1A promoter fragment
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.20; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21746877
Proper citation: RRID:Addgene_40173 Copy
Species: Homo sapiens
Genetic Insert: HIF1A promoter fragment
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL2B; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21746877
Proper citation: RRID:Addgene_40172 Copy
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