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Species: E. coli
Genetic Insert: RssB (D104N)
Vector Backbone Description: Backbone Size:5387; Vector Backbone:pSY5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229471 Copy
Species: E. coli
Genetic Insert: RssB (D104A)
Vector Backbone Description: Backbone Size:5387; Vector Backbone:pSY5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229470 Copy
Species: E. coli
Genetic Insert: RssB (N256A)
Vector Backbone Description: Backbone Size:3787; Vector Backbone:pOKD5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229473 Copy
Species: E. coli
Genetic Insert: mCherry, Cas9, SgRNA
Vector Backbone Description: Backbone Size:3150; Vector Backbone:pSIP401 (spp-based expression) derivative, lacks spp-genes for induction; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39817779
Proper citation: RRID:Addgene_225428 Copy
Species: E. coli
Genetic Insert: M1 RNA of E. coli RNase P
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40858540
Comments: When using this plasmid, please also cite the original paper:
Fukunaga J, Gouda M, Umeda K, Ohno S, Yokogawa T, Nishikawa K. Use of RNase P for efficient preparation of yeast tRNATyr transcript and its mutants. J Biochem. 2006 Jan;139(1):123-7. doi: 10.1093/jb/mvj005. PMID: 16428327
Proper citation: RRID:Addgene_246824 Copy
Species: E. coli
Genetic Insert: atpA
Vector Backbone Description: Backbone Marker:TakaraBio; Backbone Size:9138; Vector Backbone:pLVX-TetOne-bsd; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40894546
Comments: Please visit https://doi.org/10.1101/2025.08.12.670003 for bioRxiv preprint.
Proper citation: RRID:Addgene_248065 Copy
Species: E. coli
Genetic Insert: C5 protein of E. coli RNase P
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40858540
Comments: When using this plasmid, please also cite the original paper:
Fukunaga J, Gouda M, Umeda K, Ohno S, Yokogawa T, Nishikawa K. Use of RNase P for efficient preparation of yeast tRNATyr transcript and its mutants. J Biochem. 2006 Jan;139(1):123-7. doi: 10.1093/jb/mvj005. PMID: 16428327
Proper citation: RRID:Addgene_246823 Copy
Species: E. coli
Genetic Insert: E. coli tRNA arrays
Vector Backbone Description: Backbone Marker:Twist bioscience; Backbone Size:2959; Vector Backbone:pTwist vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40858540
Proper citation: RRID:Addgene_246822 Copy
Species: E. coli
Genetic Insert: IS621 Donor
Vector Backbone Description: Vector Backbone:Rep101 Ts - pSC101 Ori; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38926615
Comments: Please visit https://doi.org/10.1101/2024.01.24.577089 for bioRxiv preprint.
Proper citation: RRID:Addgene_222731 Copy
Species: E. coli
Genetic Insert: EF-Ts
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40209036
Comments: It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint.
Proper citation: RRID:Addgene_224371 Copy
Species: E. coli
Genetic Insert: MSP1D1_sfCherry
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression.
Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint.
Proper citation: RRID:Addgene_248923 Copy
Species: E. coli
Genetic Insert: MSP1D1_sfCherryL
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression.
Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint.
Proper citation: RRID:Addgene_248924 Copy
Species: E. coli
Genetic Insert: MSP1D1_sfGFP
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression.
Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint.
Proper citation: RRID:Addgene_248920 Copy
Species: E. coli
Genetic Insert: MSP1D1_mCherry
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression.
Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint.
Proper citation: RRID:Addgene_248922 Copy
Species: E. coli
Genetic Insert: MSP1E3D1_sfGFP
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression.
Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint.
Proper citation: RRID:Addgene_248927 Copy
Species: E. coli
Genetic Insert: MSP1E3D1_mCherry
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression.
Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint.
Proper citation: RRID:Addgene_248929 Copy
Species: E. coli
Genetic Insert: 2xDHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA
tag and two repeats of DHFR
Proper citation: RRID:Addgene_20218 Copy
Species: E. coli
Genetic Insert: 2xDHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Proper citation: RRID:Addgene_20215 Copy
Species: E. coli
Genetic Insert: 3xDHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA
tag and three repeats of DHFR
Proper citation: RRID:Addgene_20219 Copy
Species: E. Coli
Genetic Insert: BirA-ER
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18425138
Comments: Please email [email protected] with the intended use and/or targets for this plasmid.
Proper citation: RRID:Addgene_20856 Copy
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