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Species: Arabidopsis thaliana
Genetic Insert: AtUBC10
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104675 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC9
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104674 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC6
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104671 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC5
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104670 Copy
Species: Arabidopsis thaliana
Genetic Insert: MIEL1
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104713 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pUC19-derived; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: Level 0 cloning vector for c terminal tag, clone insert without STOP codon BpiI
Proper citation: RRID:Addgene_104642 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pUC19-derived; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: Level 0 cloning vector, clone insert BpiI
Proper citation: RRID:Addgene_104641 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2223; Vector Backbone:pICH66323; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29194629
Comments: Level 1 cloning vector defining position no.6 for cloning with BsaI
Proper citation: RRID:Addgene_104648 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2223; Vector Backbone:pICH66323; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29194629
Comments: Level 1 cloning vector defining position no.2 for cloning with BsaI
Proper citation: RRID:Addgene_104644 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2223; Vector Backbone:pICH66323; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29194629
Comments: Level 1 cloning vector defining position no.3 for cloning with BsaI
Proper citation: RRID:Addgene_104645 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2222; Vector Backbone:pICH66323; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29194629
Comments: Level 1 vector cloning for insertion of an N-terminal tag, defining position no.2 for cloning with BsaI
Proper citation: RRID:Addgene_104650 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2222; Vector Backbone:pICH66323; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29194629
Comments: Level 1 cloning vector for insertion of an N-terminal tag, defining position no.4 for cloning with BsaI
Proper citation: RRID:Addgene_104652 Copy
Species: Synthetic
Genetic Insert: N terminal HIS-Tag
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2071; Vector Backbone:pAGM3931; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone together with GOI to Level 1 cloning vector
Proper citation: RRID:Addgene_104658 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2222; Vector Backbone:pICH66323; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29194629
Comments: Level 1 cloning vector for insertion of an N-terminal tag, defining position no.6 for cloning with BsaI
Proper citation: RRID:Addgene_104654 Copy
Species: Synthetic
Genetic Insert: N terminal MBP-Tag
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pAGM1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone together with GOI to Level 1 cloning vector
Proper citation: RRID:Addgene_104656 Copy
Species: Homo sapiens
Genetic Insert: Caspase-3
Vector Backbone Description: Backbone Marker:Scott Gradia (Addgene plasmid # 29653); Vector Backbone:pET His6 TEV LIC cloning vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28893998
Comments: LOV2 domain was inserted into the intersubunit linker of the caspase, with linker variation N7C-7
Proper citation: RRID:Addgene_104629 Copy
Species: Homo sapiens
Genetic Insert: Caspase-3
Vector Backbone Description: Backbone Marker:Scott Gradia (Addgene plasmid # 29653); Vector Backbone:pET His6 TEV LIC cloning vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28893998
Comments: Please note that during Addgene's quality control process 2 mismatches were found in lacI which result in mutations S219F and Q311P.
LOV2 domain was inserted into the intersubunit linker of the caspase, with linker variation N7C-7 and mutation C450A, which is a dark-locked and light-insensitive mutation
Proper citation: RRID:Addgene_104628 Copy
Species: Synthetic
Genetic Insert: crRNA to ligD
Vector Backbone Description: Vector Backbone:pMN016; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30039929
Comments: Shuttle vector Mycobacterium smegmatis/E.coli
Proper citation: RRID:Addgene_104623 Copy
Species: Arabidopsis thaliana
Genetic Insert: HIS-Ubq_UBA1_HIS-Ubq_GST-AvrPtoB_EL4
Vector Backbone Description: Backbone Size:5190; Vector Backbone:pET-28GG; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29194629
Comments: Level 2 construct to be used as negative control, contains a Ubq-E1-E3 combination without E2
Proper citation: RRID:Addgene_104637 Copy
Species: Arabidopsis thaliana
Genetic Insert: HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB-F/A_EL4
Vector Backbone Description: Backbone Size:5190; Vector Backbone:pET-28GG; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29194629
Comments: Level 2 construct to be used as negative control, contains a Ubq-E1-E2-E3 combination with mutated E3
Proper citation: RRID:Addgene_104636 Copy
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