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  • Organism:e. coli (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pBEL1223
 
Resource Report
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RRID:Addgene_195688 MlaD E. coli Ampicillin PMID:37100290 Please visit https://www.biorxiv.org/content/10.1101/2022.12.09.519820v1 for bioRxiv preprint. Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 6xHis-TEV-MlaDdelta173-183 2026-08-15 01:26:20 0
pBEL1954
 
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RRID:Addgene_195689 MlaD E. coli Ampicillin PMID:37100290 Please visit https://www.biorxiv.org/content/10.1101/2022.12.09.519820v1 for bioRxiv preprint. Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 6xHis-TEV-MlaD(F118A/L123A) 2026-08-15 01:26:21 0
pBEL2576
 
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RRID:Addgene_195686 MlaC E. coli Ampicillin PMID:37100290 Please visit https://www.biorxiv.org/content/10.1101/2022.12.09.519820v1 for bioRxiv preprint. Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 6xHis-TEV-MlaC(Y164R) 2026-08-15 01:26:21 0
pBEL2574
 
Resource Report
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RRID:Addgene_195685 MlaC E. coli Ampicillin PMID:37100290 Please visit https://www.biorxiv.org/content/10.1101/2022.12.09.519820v1 for bioRxiv preprint. Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 6xHis-TEV-MlaC(Y82K) 2026-08-15 01:26:20 0
pJLG039
 
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RRID:Addgene_192982 T7 polymerase E. coli Tetracycline PMID:36645284 The T7 polymerase gene (1) was cloned from the chromosome of BL21(DE3). Backbone Size:8751; Vector Backbone:pSW213; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:26:34 0
pTETDuet_M42FG121VDHFR_R127ATS
 
Resource Report
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1+ mentions
RRID:Addgene_191305 folA, thyA E. coli Chloramphenicol PMID:38637543 Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint. Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol M42F/G121V DHFR, R127A TYMS 2026-08-15 01:26:36 1
pFV2-HA
 
Resource Report
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RRID:Addgene_187082 atpBEFHAGDC E. coli Ampicillin PMID:19362069 Please note: Plasmid contains a N419K mutation in ATPase alpha and a D6E mutation in ATPase gamma. These mutation are not known to affect plasmid function. Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin All Cys codons changed to Ala. 2026-08-15 01:26:45 0
3xFLAG-TurboID
 
Resource Report
Resource Website
RRID:Addgene_236117 TurboID E. coli Ampicillin Addgene NGS finds the 3x-FLAG tag C-terminal on the insert. Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:36:26 0
pATLIC
 
Resource Report
Resource Website
RRID:Addgene_110287 yfaL E. coli Ampicillin PMID:22344647 Addgene QC finds S92G, G99S, and K330R mutations in yfaL as well as a stop codon inserted before the His tag. The depositor notes these are not of functional concern Backbone Size:3986; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:01:39 0
PT7lacO-EF-Ts-His6
 
Resource Report
Resource Website
RRID:Addgene_224371 EF-Ts E. coli Ampicillin PMID:40209036 It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint. Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-18 09:14:32 0
pMSP1D1_sfCherry
 
Resource Report
Resource Website
RRID:Addgene_248923 MSP1D1_sfCherry E. coli Kanamycin Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression. Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint. Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-18 09:16:45 0
pMSP1D1_sfCherryL
 
Resource Report
Resource Website
RRID:Addgene_248924 MSP1D1_sfCherryL E. coli Kanamycin Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression. Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint. Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-18 09:16:45 0
pMSP1D1_sfGFP
 
Resource Report
Resource Website
RRID:Addgene_248920 MSP1D1_sfGFP E. coli Kanamycin Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression. Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint. Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-18 09:16:45 0
pMSP1D1_mCherry
 
Resource Report
Resource Website
RRID:Addgene_248922 MSP1D1_mCherry E. coli Kanamycin Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression. Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint. Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-18 09:16:45 0
pMSP1E3D1_sfGFP
 
Resource Report
Resource Website
RRID:Addgene_248927 MSP1E3D1_sfGFP E. coli Kanamycin Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression. Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint. Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-18 09:16:45 0
pMSP1E3D1_mCherry
 
Resource Report
Resource Website
RRID:Addgene_248929 MSP1E3D1_mCherry E. coli Kanamycin Depositor recommends growing plasmid in the E. coli strain BL21 (DE3) for protein expression. Please visit https://doi.org/10.64898/2026.04.07.716332 for bioRxiv preprint. Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-18 09:16:45 0
pRG/III-hs-MBP
 
Resource Report
Resource Website
RRID:Addgene_121676 maltose binding protein E. coli Ampicillin PMID:8760379 pRG/III-hs-MBP is a derivative of NEB's pMal-p2 vector, see Tucker Grisshammer Biochemical Journal 317, 891, 1996. Internal plasmid code # 591 Vector Backbone:pRG/III-hs-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:03:38 0
No-repeats
 
Resource Report
Resource Website
RRID:Addgene_122487 LacZ E. coli Ampicillin PMID:28761084 Vector Backbone:pUASg; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:03:45 0
pAT-9218
 
Resource Report
Resource Website
RRID:Addgene_124225 SpCas9 E. coli Chloramphenicol PMID:33450024 Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol 2026-08-15 01:04:04 0
pSCM001
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_124605 mCherry-pHluorin E. coli Ampicillin PMID:30846797 Backbone Marker:Invitrogen; Backbone Size:4127; Vector Backbone:pBAD TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:08 1

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